##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547118_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	562435
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429930569754728	31.0	31.0	33.0	30.0	34.0
2	31.780276831989475	31.0	31.0	34.0	30.0	34.0
3	31.888029727879665	31.0	31.0	34.0	30.0	34.0
4	35.63410349640403	37.0	35.0	37.0	33.0	37.0
5	35.561732466862836	37.0	35.0	37.0	33.0	37.0
6	35.60904993465912	37.0	35.0	37.0	33.0	37.0
7	35.974994443802395	37.0	35.0	37.0	35.0	37.0
8	34.66507774231689	37.0	35.0	37.0	33.0	37.0
9	36.99401175246918	39.0	37.0	39.0	33.0	39.0
10	36.89894298896761	39.0	37.0	39.0	32.0	39.0
11	36.746742290220205	39.0	35.0	39.0	32.0	39.0
12	36.05467298443376	37.0	35.0	39.0	32.0	39.0
13	35.719292007076376	37.0	35.0	39.0	31.0	39.0
14	36.72423480046583	39.0	35.0	41.0	31.0	41.0
15	37.05667854952128	39.0	35.0	41.0	32.0	41.0
16	37.20356663436664	39.0	35.0	41.0	32.0	41.0
17	37.13326339932615	38.0	35.0	41.0	32.0	41.0
18	37.08341052743873	38.0	35.0	41.0	32.0	41.0
19	37.01041720376577	38.0	35.0	41.0	32.0	41.0
20	36.83479690986514	38.0	35.0	41.0	32.0	41.0
21	36.70168819508032	38.0	35.0	41.0	32.0	41.0
22	36.583048707850686	38.0	35.0	41.0	31.0	41.0
23	36.531695218114095	38.0	35.0	40.0	31.0	41.0
24	36.40579978130806	38.0	35.0	40.0	31.0	41.0
25	36.30753420395246	37.0	35.0	40.0	31.0	41.0
26	36.181798785637454	37.0	35.0	40.0	31.0	41.0
27	36.0803523962769	37.0	35.0	40.0	31.0	41.0
28	36.11652902113133	37.0	35.0	40.0	31.0	41.0
29	36.11930800892548	37.0	35.0	40.0	31.0	41.0
30	36.094185105834455	37.0	35.0	40.0	31.0	41.0
31	35.9458639665028	37.0	35.0	40.0	30.0	41.0
32	35.77071483815907	37.0	35.0	40.0	30.0	41.0
33	35.64165103523074	37.0	35.0	40.0	30.0	41.0
34	35.53755722883533	37.0	35.0	40.0	29.0	41.0
35	35.369048867869175	37.0	35.0	40.0	29.0	41.0
36	35.22046636500218	37.0	34.0	40.0	28.0	41.0
37	35.13758034261737	37.0	34.0	40.0	27.0	41.0
38	35.07027656529199	36.0	34.0	40.0	27.0	41.0
39	35.0247566385449	36.0	34.0	40.0	27.0	41.0
40	34.849355036582004	36.0	34.0	40.0	26.0	41.0
41	34.8187274973997	36.0	34.0	40.0	26.0	41.0
42	34.75456897241459	36.0	34.0	40.0	26.0	41.0
43	34.66527687643906	36.0	34.0	40.0	26.0	41.0
44	34.5467209544214	35.0	34.0	40.0	26.0	41.0
45	34.41117106865682	35.0	34.0	40.0	25.0	41.0
46	34.35542951629966	35.0	34.0	40.0	24.0	41.0
47	34.30522993768169	35.0	34.0	40.0	25.0	41.0
48	34.28745543929521	35.0	34.0	40.0	25.0	41.0
49	34.25132504200485	35.0	34.0	40.0	25.0	41.0
50	34.09056868793727	35.0	34.0	40.0	24.0	41.0
51	33.739507676442614	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	5.0
12	1.0
13	9.0
14	11.0
15	15.0
16	34.0
17	121.0
18	269.0
19	495.0
20	950.0
21	1418.0
22	2099.0
23	3108.0
24	4539.0
25	6364.0
26	8379.0
27	9983.0
28	10911.0
29	12259.0
30	14321.0
31	17323.0
32	21244.0
33	27971.0
34	50698.0
35	88637.0
36	40698.0
37	48934.0
38	72046.0
39	119504.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.778836665570246	23.652333158498315	28.62001831322731	13.948811862704137
2	29.54119142656485	26.44109986042832	29.965418226106127	14.05229048690071
3	28.575568732386852	25.75230915572466	32.0717949629735	13.600327148914987
4	24.513766035186286	28.222461262190297	31.65574688630686	15.608025816316552
5	22.799790197978435	32.62065838719141	30.25949665294656	14.320054761883597
6	21.241921288682246	41.22663063287313	27.12989056513197	10.40155751331265
7	86.05527749873319	4.6904975686079275	7.3868091423897875	1.8674157902690978
8	84.30467520691279	6.1420430805337505	6.146488038617796	3.406793673935655
9	82.28844221998986	5.1232586876705755	9.34010152284264	3.2481975694969196
10	50.581667214878166	21.725710526549737	14.161280859121497	13.531341399450604
11	43.244997199676405	21.131686328197922	21.17560251406829	14.447713958057376
12	37.88917830504858	20.634562216078304	25.200067563362875	16.27619191551024
13	21.473059109052603	37.84704010241183	26.03696427142692	14.642936517108643
14	16.060344750949	39.375038893383234	28.800305813116182	15.764310542551584
15	13.781859237067396	23.30651541955959	47.391431898797194	15.520193444575817
16	16.084880919572928	18.815329771440258	45.43831731666771	19.661471992319115
17	16.490972290131303	20.017957630659545	29.224710410980826	34.26635966822833
18	21.56551423720074	23.29726990674478	33.10106945691502	22.036146399139454
19	27.644438912941048	25.665365775600733	25.681901019673386	21.00829429178483
20	30.013423773413816	23.082489532123713	24.70952198920764	22.19456470525483
21	23.255487300754755	27.893889960617674	27.352316267657596	21.49830647096998
22	23.38154631201828	24.834869807177718	24.984042600478276	26.799541280325727
23	19.92656929245157	30.635895703503518	24.43624596620054	25.00128903784437
24	21.368335896592495	23.2305955354841	36.93155653542187	18.469512032501534
25	19.201152133135384	24.40406446967205	33.70931752113577	22.68546587605679
26	17.945006978584193	32.369784952927894	27.503444842515133	22.181763225972777
27	19.621467369562705	30.85725461608897	27.737783032706005	21.783494981642324
28	16.80371954092473	27.881266279658984	35.94495363908719	19.370060540329103
29	17.00605403291047	24.619556037586566	34.518122094108655	23.856267835394313
30	20.135660120725063	27.95576377714758	30.689768595482143	21.218807506645213
31	28.388524896210228	25.073475157129266	24.679651870882857	21.858348075777645
32	28.951612186297083	26.14613244197107	24.94706054921902	19.955194822512823
33	26.61356423408927	25.214113630908457	25.41751491283437	22.754807222167894
34	21.121729622089664	26.548134451092125	28.543387235858365	23.786748690959843
35	20.771111328420172	24.609065936508216	31.62516557468863	22.994657160382978
36	29.85571666059189	24.363170855298836	25.883346520042316	19.89776596406696
37	21.47003653755545	28.612550783646114	29.909945149217243	20.007467529581195
38	21.756647434814692	31.269568927965008	24.969818734609333	22.003964902610967
39	21.401939779707877	28.979882119711608	27.960208735231628	21.657969365348883
40	23.856445633717673	25.08040929174038	26.711708908584992	24.351436165956954
41	17.965987180740886	23.93752166917066	28.612550783646114	29.483940366442347
42	23.0928018348787	25.804581862793036	24.846071101549512	26.25654520077876
43	22.926915999182125	24.847137891489684	27.601589517010854	24.624356592317334
44	21.48479379839448	28.683492314667475	27.81405851342822	22.01765537350983
45	19.46393805506414	31.80207490643363	25.286477548516718	23.44750948998551
46	23.123205348173567	28.52614079849227	26.70175220247673	21.648901650857432
47	22.41752380275054	25.322392809835804	28.414839048067776	23.845244339345882
48	23.959746459590885	22.61310195844853	30.155840230426627	23.271311351533956
49	21.87826148799417	22.688844044200664	31.304595197667282	24.12829927013788
50	20.229182038813374	29.544391796385362	27.83574990887836	22.390676255922905
51	19.596575604292052	29.842026189693033	25.327371162889932	25.234027043124986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	606.0
1	794.5
2	983.0
3	2988.0
4	4993.0
5	3485.0
6	1977.0
7	2149.5
8	2322.0
9	2617.5
10	2913.0
11	3037.0
12	3161.0
13	3115.0
14	3069.0
15	3177.5
16	3286.0
17	3108.0
18	2930.0
19	2843.5
20	2757.0
21	2789.0
22	2821.0
23	2779.5
24	2738.0
25	2834.5
26	3184.5
27	3438.0
28	4035.5
29	4633.0
30	5451.0
31	6269.0
32	6754.5
33	7240.0
34	8597.0
35	9954.0
36	11693.0
37	13432.0
38	15963.0
39	18494.0
40	25347.5
41	32201.0
42	39470.0
43	46739.0
44	48649.0
45	50559.0
46	51355.5
47	52152.0
48	52734.5
49	53317.0
50	49909.0
51	46501.0
52	42279.0
53	38057.0
54	34361.0
55	30665.0
56	27510.0
57	24355.0
58	23036.0
59	21717.0
60	20018.0
61	18319.0
62	16373.0
63	14427.0
64	12856.0
65	11285.0
66	9150.5
67	7016.0
68	6047.5
69	5079.0
70	4324.5
71	3570.0
72	2878.0
73	2186.0
74	1987.0
75	1284.0
76	780.0
77	573.5
78	367.0
79	301.0
80	235.0
81	176.0
82	117.0
83	69.0
84	21.0
85	17.0
86	13.0
87	8.0
88	3.0
89	3.5
90	4.0
91	5.5
92	7.0
93	6.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562435.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.454153075277354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78140953495935	21.628768768588895
2	6.697218807439792	3.67732940636157
3	2.397738672051249	1.9748365361102174
4	1.2867636685349182	1.4130802691065238
5	0.8921983534942106	1.224727508517024
6	0.6344171718699776	1.0450431690061746
7	0.5319249974828568	1.0222485213822612
8	0.4839967737056596	1.0630177210604443
9	0.41949150816575076	1.0365105671065336
>10	6.923503911037006	47.47740459116335
>50	0.9035076949975821	15.425891929652025
>100	0.04389776328124583	1.816757719273834
>500	0.0026207619869400497	0.4029244842745929
>1k	0.0013103809934700249	0.7914588083965217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2966	0.5273498270911305	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1434	0.2549627957008365	No Hit
GAGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	667	0.11859148168232773	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09814467449571951	0.0
2	0.0	0.0	0.0	0.31523642732049034	0.0
3	0.0	0.0	0.0	0.45000755642874285	0.0
4	0.0	0.0	0.0	0.8788571123774302	0.0
5	0.0	0.0	0.0	1.3375767866509018	0.0
6	0.0	0.0	0.0	2.156515864055402	0.0
7	0.0	0.0	0.0	2.5016224097006767	0.0
8	0.0	0.0	0.0	3.60468320783735	0.0
9	0.0	0.0	0.0	4.041355890013957	0.0
10	0.0	0.0	0.0	4.760550108012481	0.0
11	0.0	0.0	0.0	5.338394658938366	0.0
12	0.0	0.0	0.0	5.858632553095024	0.0
13	0.0	0.0	0.0	6.072701734422645	0.0
14	0.0	0.0	0.0	6.148799416821499	0.0
15	0.0	0.0	0.0	6.240898948322918	0.0
16	0.0	0.0	0.0	6.441277658751678	0.0
17	0.0	0.0	0.0	6.670459697565052	0.0
18	0.0	0.0	0.0	7.010943486802919	0.0
19	0.0	0.0	0.0	7.1405584645336795	0.0
20	0.0	0.0	0.0	7.297909980708882	0.0
21	0.0	0.0	0.0	7.496688506227386	0.0
22	0.0	0.0	0.0	7.710224292584921	0.0
23	0.0	0.0	0.0	7.958252953674647	0.0
24	0.0	0.0	0.0	8.134451092126202	0.0
25	1.777983233618107E-4	0.0	0.0	8.280956910576334	0.0
26	1.777983233618107E-4	0.0	0.0	8.424795754176039	0.0
27	1.777983233618107E-4	0.0	0.0	8.55850009334412	0.0
28	1.777983233618107E-4	0.0	0.0	8.694871407362628	0.0
29	1.777983233618107E-4	0.0	0.0	8.839065847609056	0.0
30	1.777983233618107E-4	0.0	0.0	9.053846222230124	0.0
31	1.777983233618107E-4	0.0	0.0	9.208530763554899	0.0
32	1.777983233618107E-4	0.0	0.0	9.357703556855459	0.0
33	1.777983233618107E-4	0.0	0.0	9.496030652430948	0.0
34	1.777983233618107E-4	0.0	0.0	9.670806404295607	0.0
35	1.777983233618107E-4	0.0	0.0	9.873674291251433	0.0
36	1.777983233618107E-4	0.0	0.0	10.038137740361108	0.0
37	1.777983233618107E-4	0.0	0.0	10.198511828033462	0.0
38	1.777983233618107E-4	0.0	0.0	10.364042067083307	0.0
39	1.777983233618107E-4	0.0	0.0	10.51943780170153	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.0302107E-4	45.0	1
ATTAGCG	20	7.0302107E-4	45.0	1
GTCGAAT	20	7.0302107E-4	45.0	13
ATAGGAC	20	7.0302107E-4	45.0	26
TGAAGCG	20	7.0302107E-4	45.0	1
GGCGTAT	25	3.888256E-5	45.0	8
TAGCGAA	20	7.0302107E-4	45.0	29
TAACGCG	20	7.0302107E-4	45.0	1
TGTTACG	45	3.8380676E-10	45.0	1
CGAATAT	35	1.2104465E-7	45.0	14
CCGACAG	20	7.0302107E-4	45.0	12
TGTAGCG	45	3.8380676E-10	45.0	1
GTCCCGT	20	7.0302107E-4	45.0	27
GCTACGA	35	1.2104465E-7	45.0	10
CTTTACG	25	3.888256E-5	45.0	1
CGCGTAA	35	1.2104465E-7	45.0	31
CGACTAA	35	1.2104465E-7	45.0	30
TAATGCG	20	7.0302107E-4	45.0	1
TCGAATC	25	3.888256E-5	45.0	14
GCGTAAC	35	1.2104465E-7	45.0	32
>>END_MODULE