FastQCFastQC Report
Thu 26 May 2016
SRR1547117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547117_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1199802
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.45049099768128414No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.03341E-445.00000418
CATAGCG351.2119926E-745.0000041
CGTAGAC207.03341E-445.00000428
CGAATTA207.03341E-445.00000417
CGGTCTA700.041.78571731
TACGTAG650.041.5384641
CACGATA501.0822987E-940.50000422
CGTTTTT36700.039.1144451
TAAACGG750.039.0000042
CATGCGG1050.038.571432
CGTTCGA700.038.5714314
TCTAGCG356.248518E-638.571431
AGGGCGA12700.038.444886
GGGCGAT28250.038.1504447
GTTAGCG659.094947E-1238.0769271
CGTTAGG1300.038.0769272
GGCGATA6400.037.617198
TATGGGA8800.037.5852284
TATAGCG601.5643309E-1037.5000041
GTACGTC301.1399516E-437.50000416