##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547117_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1199802
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42105197357564	31.0	31.0	33.0	30.0	34.0
2	31.784892007181185	31.0	31.0	34.0	30.0	34.0
3	31.907365548648862	31.0	31.0	34.0	30.0	34.0
4	35.65821610565743	37.0	35.0	37.0	33.0	37.0
5	35.596265050399985	37.0	35.0	37.0	33.0	37.0
6	35.64324030131638	37.0	35.0	37.0	33.0	37.0
7	35.987259564494806	37.0	35.0	37.0	35.0	37.0
8	34.66111158341126	37.0	35.0	37.0	33.0	37.0
9	36.96876067884534	39.0	37.0	39.0	33.0	39.0
10	36.872381442938085	39.0	37.0	39.0	32.0	39.0
11	36.79051710198849	39.0	37.0	39.0	32.0	39.0
12	36.44097026009292	39.0	35.0	39.0	32.0	39.0
13	36.33949351643021	39.0	35.0	39.0	32.0	39.0
14	37.394297559097254	40.0	35.0	41.0	32.0	41.0
15	37.558197102521916	40.0	35.0	41.0	32.0	41.0
16	37.60294781972359	40.0	35.0	41.0	33.0	41.0
17	37.4866986386087	39.0	35.0	41.0	32.0	41.0
18	37.43349485998523	39.0	36.0	41.0	32.0	41.0
19	37.388390751140605	39.0	35.0	41.0	32.0	41.0
20	37.288782649137104	39.0	35.0	41.0	32.0	41.0
21	37.18662412631417	39.0	35.0	41.0	32.0	41.0
22	37.10067411122835	39.0	35.0	41.0	32.0	41.0
23	37.02404896807973	39.0	35.0	41.0	32.0	41.0
24	36.88409920970293	39.0	35.0	41.0	31.0	41.0
25	36.75395856983069	38.0	35.0	41.0	31.0	41.0
26	36.64061653506162	38.0	35.0	41.0	31.0	41.0
27	36.550925902773955	38.0	35.0	41.0	31.0	41.0
28	36.57470232588377	38.0	35.0	41.0	31.0	41.0
29	36.52730283830165	38.0	35.0	41.0	31.0	41.0
30	36.47287469099068	38.0	35.0	40.0	31.0	41.0
31	36.375872018883115	38.0	35.0	40.0	30.0	41.0
32	36.31744321146323	38.0	35.0	41.0	30.0	41.0
33	36.206068167914374	38.0	35.0	41.0	30.0	41.0
34	36.07289036024277	38.0	35.0	41.0	30.0	41.0
35	35.96550514168171	38.0	35.0	41.0	29.0	41.0
36	35.83654553001245	38.0	35.0	41.0	29.0	41.0
37	35.802757454980075	38.0	35.0	41.0	29.0	41.0
38	35.70711167342611	38.0	35.0	40.0	29.0	41.0
39	35.6132978608137	38.0	35.0	40.0	28.0	41.0
40	35.44573771338938	38.0	34.0	40.0	27.0	41.0
41	35.34365837029777	38.0	34.0	40.0	27.0	41.0
42	35.27662981058541	38.0	34.0	40.0	27.0	41.0
43	35.191446588687135	37.0	34.0	40.0	27.0	41.0
44	35.08297452412982	37.0	34.0	40.0	27.0	41.0
45	34.95519760760526	37.0	34.0	40.0	26.0	41.0
46	34.877345595356566	37.0	34.0	40.0	26.0	41.0
47	34.7979466611991	37.0	34.0	40.0	26.0	41.0
48	34.71622817764931	36.0	34.0	40.0	26.0	41.0
49	34.63003062171925	36.0	34.0	40.0	26.0	41.0
50	34.52630100633271	36.0	34.0	40.0	26.0	41.0
51	34.16200506416892	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	5.0
12	2.0
13	2.0
14	18.0
15	38.0
16	106.0
17	262.0
18	540.0
19	1053.0
20	1772.0
21	2922.0
22	4232.0
23	6059.0
24	8639.0
25	12042.0
26	15910.0
27	18683.0
28	21101.0
29	24432.0
30	28707.0
31	34409.0
32	42668.0
33	54943.0
34	90961.0
35	132412.0
36	95409.0
37	124800.0
38	181222.0
39	296219.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.84083040368328	23.698243543518014	27.077051046756047	13.383875006042665
2	32.018949793382575	25.23416363700011	28.797001505248364	13.949885064368955
3	30.381679643807896	25.509709102001832	30.54970736838245	13.558903885807824
4	26.078886349581015	28.587383584958186	29.740157125925776	15.593572939535022
5	22.996294388574114	32.96443913245686	28.704652934400844	15.334613544568187
6	21.812765773019215	41.366908873297426	26.278502619598896	10.541822734084457
7	87.78406770450458	4.110178179399601	6.398472414615078	1.7072817014807444
8	85.031196814141	6.365133580374096	5.965234263653503	2.638435341831402
9	82.4910276862349	5.4712360872877355	8.528240493014682	3.5094957334626877
10	43.550102433568206	27.101638437008774	14.944215795606276	14.404043333816746
11	35.91909331706398	25.8105920810267	21.90236389004186	16.367950711867458
12	32.41143121948455	22.67765848031592	26.55254783705978	18.358362463139752
13	24.62597995335897	28.39501851138771	28.021373526631894	18.957628008621423
14	20.740422169657993	30.382013032150308	29.130139806401388	19.747424991790314
15	20.46395988671464	23.732249154443817	37.28356845546182	18.520222503379724
16	23.511546071768507	21.630068961378626	34.777071550139105	20.08131341671376
17	22.94520262509981	22.643319481047705	28.51637186802489	25.895106025827598
18	25.287589118871278	23.30851257124092	30.332171474960035	21.071726834927762
19	27.885184388757477	26.123060304950318	25.52629517203672	20.465460134255487
20	30.284913677423443	24.537632042620366	24.82801328885933	20.349440991096866
21	27.265748848560012	26.53596176702489	26.498038843075772	19.70025054133932
22	25.860516985302574	24.036799405235197	26.023210496398576	24.079473113063653
23	24.052301963157255	27.88726806589754	25.88743809395217	22.172991876993038
24	23.8368497468749	24.847183118547893	30.843089109703104	20.472878024874106
25	23.91236220643073	25.322428200653107	28.6861498813971	22.079059711519065
26	21.844270971376943	28.021290179546295	26.931527035294156	23.202911813782606
27	22.59972895527762	26.74532964605827	27.4824512711264	23.17249012753771
28	21.187579283915177	27.42569190583113	30.271911532069456	21.114817278184233
29	22.50646356648847	25.623227832592377	28.8761812365707	22.99412736434845
30	24.150651524168154	26.243746884902674	28.46678035209143	21.13882123883774
31	26.812840785396258	25.741163958719852	25.49929071630152	21.946704539582367
32	28.098636274985374	25.09572412781442	26.241246472334602	20.564393124865603
33	26.378602469407454	25.217910955307627	25.318844275972204	23.08464229931272
34	22.708663596160033	26.30217319191	28.044377322258175	22.944785889671795
35	22.64106910973644	26.50462326283837	28.988199719620404	21.86610790780479
36	26.852097262715013	27.168566146747548	25.335763734349502	20.643572856187937
37	23.23891775476287	29.31342004764119	26.356265450465994	21.09139674712994
38	23.628732074125562	29.052627016791106	25.538463846534675	21.780177062548653
39	23.64515144998925	27.838510020820102	25.476953697360067	23.039384831830585
40	25.6816541395997	24.998124690573945	26.729743741050605	22.59047742877575
41	21.21816766433128	25.32826249664528	27.104472237919257	26.349097601104184
42	23.520880945355984	26.31209149509669	25.81659307119008	24.350434488357244
43	22.382943185625628	25.264501976159398	27.45061268442626	24.90194215378871
44	22.565223261838202	26.41435836913091	27.67598320389531	23.34443516513558
45	21.87502604596425	27.57521657740194	25.87085202391728	24.678905352716534
46	23.204245367152247	27.453779873679157	26.5057067749512	22.836267984217397
47	23.20232838418339	26.231578210404717	27.526375185238898	23.039718220172993
48	23.351102931983778	25.442364656835043	28.545543347985753	22.660989063195426
49	23.292676624976455	24.91886161216601	28.536541862740684	23.251919900116853
50	22.071475126729243	27.135894089191382	27.967114573904695	22.82551621017468
51	21.477377100554925	27.458530657558498	26.372851520500884	24.691240721385697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1103.0
1	1593.5
2	2084.0
3	6099.5
4	10115.0
5	7193.5
6	4272.0
7	4402.5
8	4533.0
9	4872.0
10	5211.0
11	5437.5
12	5664.0
13	5609.5
14	5555.0
15	5547.5
16	5540.0
17	5423.5
18	5307.0
19	5137.0
20	4967.0
21	4987.5
22	5008.0
23	5527.5
24	6047.0
25	6276.0
26	7940.5
27	9376.0
28	11281.0
29	13186.0
30	14631.0
31	16076.0
32	17596.5
33	19117.0
34	23312.5
35	27508.0
36	29495.5
37	31483.0
38	35783.0
39	40083.0
40	48590.0
41	57097.0
42	63301.5
43	69506.0
44	75336.5
45	81167.0
46	84092.0
47	87017.0
48	89714.5
49	92412.0
50	91150.5
51	89889.0
52	86364.0
53	82839.0
54	78037.0
55	73235.0
56	69082.5
57	64930.0
58	62840.5
59	60751.0
60	58316.5
61	55882.0
62	50826.0
63	45770.0
64	40695.5
65	35621.0
66	30073.5
67	24526.0
68	20951.0
69	17376.0
70	15012.0
71	12648.0
72	10324.5
73	8001.0
74	6902.5
75	4667.0
76	3530.0
77	2533.0
78	1536.0
79	1216.0
80	896.0
81	613.0
82	330.0
83	260.5
84	191.0
85	125.0
86	59.0
87	42.5
88	26.0
89	17.0
90	8.0
91	8.5
92	9.0
93	6.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1199802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.44917975000627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07990165700372	20.122509942888907
2	6.369188784782673	3.3691963806088174
3	2.5505752896967175	2.023818729093384
4	1.6189160715204958	1.7127600870327842
5	1.1873372919492504	1.5702048729325697
6	0.9698410734351496	1.5390900528135179
7	0.7706282492463997	1.426773955932546
8	0.6840935074528438	1.4474969715546013
9	0.6248161365308194	1.4873286875227292
>10	8.435617055081071	51.85997836955646
>50	0.6691319528351328	10.914846235951767
>100	0.03735436588265282	1.6812193092144747
>500	0.002273744010233197	0.38042000619199323
>1k	0.0	0.0
>5k	3.248205728904567E-4	0.464356398705448
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5405	0.45049099768128414	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029171479960860208	0.0
2	0.0	0.0	0.0	0.09534906592921165	0.0
3	0.0	0.0	0.0	0.1593596276718992	0.0
4	0.0	0.0	0.0	0.30279996199372894	0.0
5	0.0	0.0	0.0	0.5130013118831274	0.0
6	0.0	0.0	0.0	1.01600097349396	0.0
7	0.0	0.0	0.0	1.2578742159122922	0.0
8	0.0	0.0	0.0	1.9383198227707572	0.0
9	0.0	0.0	0.0	2.305713776106391	0.0
10	0.0	0.0	0.0	2.8253828548377147	0.0
11	0.0	0.0	0.0	3.2219482881342087	0.0
12	0.0	0.0	0.0	3.53975072553638	0.0
13	0.0	0.0	0.0	3.73269922870607	0.0
14	0.0	0.0	0.0	3.833382508113839	0.0
15	0.0	0.0	0.0	3.9138124457202106	0.0
16	0.0	0.0	0.0	4.055669185415594	0.0
17	0.0	0.0	0.0	4.204860468643993	0.0
18	0.0	0.0	0.0	4.40380996197706	0.0
19	0.0	0.0	0.0	4.508160513151337	0.0
20	0.0	0.0	0.0	4.622429367512306	0.0
21	0.0	0.0	0.0	4.760952223783591	0.0
22	0.0	0.0	0.0	4.916144497175368	0.0
23	0.0	0.0	0.0	5.0938404836798075	0.0
24	0.0	0.0	0.0	5.22994627446862	0.0
25	0.0	0.0	0.0	5.357467315440381	0.0
26	0.0	0.0	0.0	5.473653152770207	0.0
27	0.0	0.0	0.0	5.595256550664193	0.0
28	0.0	0.0	0.0	5.715193006846129	0.0
29	0.0	0.0	0.0	5.849298467580484	0.0
30	0.0	0.0	0.0	6.042663706178186	0.0
31	0.0	0.0	0.0	6.18660412301363	0.0
32	0.0	0.0	0.0	6.347797386568784	0.0
33	0.0	0.0	0.0	6.498822305680437	0.0
34	0.0	0.0	0.0	6.654014579072213	0.0
35	0.0	0.0	0.0	6.823625898273215	0.0
36	0.0	0.0	0.0	6.971733669388782	0.0
37	0.0	0.0	0.0	7.132760238772731	0.0
38	0.0	0.0	0.0	7.331376343763387	0.0
39	0.0	0.0	0.0	7.618590400749457	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.03341E-4	45.000004	18
CATAGCG	35	1.2119926E-7	45.000004	1
CGTAGAC	20	7.03341E-4	45.000004	28
CGAATTA	20	7.03341E-4	45.000004	17
CGGTCTA	70	0.0	41.785717	31
TACGTAG	65	0.0	41.538464	1
CACGATA	50	1.0822987E-9	40.500004	22
CGTTTTT	3670	0.0	39.114445	1
TAAACGG	75	0.0	39.000004	2
CATGCGG	105	0.0	38.57143	2
CGTTCGA	70	0.0	38.57143	14
TCTAGCG	35	6.248518E-6	38.57143	1
AGGGCGA	1270	0.0	38.44488	6
GGGCGAT	2825	0.0	38.150444	7
GTTAGCG	65	9.094947E-12	38.076927	1
CGTTAGG	130	0.0	38.076927	2
GGCGATA	640	0.0	37.61719	8
TATGGGA	880	0.0	37.585228	4
TATAGCG	60	1.5643309E-10	37.500004	1
GTACGTC	30	1.1399516E-4	37.500004	16
>>END_MODULE