FastQCFastQC Report
Thu 26 May 2016
SRR1547116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547116_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences999647
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85930.8596034400143251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT302.1652158E-645.00000426
CGTTTTT59650.042.1709981
GTTAGCG451.9279469E-840.01
CGTTAGG900.040.02
CGTAAGG1750.039.8571432
TAGGGCG4650.039.193555
ATACTAT9000.039.045
TATACTA9250.037.94594644
GTTGATC5600.037.7678616
ATTATAC9350.037.54010442
CATCGAA301.1398153E-437.50000424
ATAGTCG301.1398153E-437.5000041
TACGGGT2200.036.818184
AGGGCGA12350.036.619436
GTAGGGA9600.036.5625044
AATGCGG1050.036.428572
CGAATAT1050.036.4285714
ACTAATC10450.036.38755810
TATGGGT3650.036.3698654
TAAGGGA10150.036.354684
Produced by FastQC (version 0.11.5)