##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547116_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	999647
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.419366036210782	31.0	31.0	33.0	30.0	34.0
2	31.790736129853837	31.0	31.0	34.0	30.0	34.0
3	31.87187477179444	31.0	31.0	34.0	30.0	34.0
4	35.65784321865618	37.0	35.0	37.0	33.0	37.0
5	35.60725636149561	37.0	35.0	37.0	33.0	37.0
6	35.653667744713886	37.0	35.0	37.0	33.0	37.0
7	36.01734912424086	37.0	35.0	37.0	35.0	37.0
8	34.69981903611975	37.0	35.0	37.0	33.0	37.0
9	37.01768024112512	39.0	37.0	39.0	34.0	39.0
10	36.81998245380619	39.0	37.0	39.0	32.0	39.0
11	36.81535381989843	39.0	37.0	39.0	32.0	39.0
12	36.661490506148674	39.0	35.0	39.0	32.0	39.0
13	36.64959730784967	39.0	35.0	39.0	32.0	39.0
14	37.76948762913308	40.0	36.0	41.0	33.0	41.0
15	37.88820253549503	40.0	36.0	41.0	33.0	41.0
16	37.894515764064714	40.0	36.0	41.0	33.0	41.0
17	37.791210297234926	40.0	36.0	41.0	33.0	41.0
18	37.700979445744345	39.0	36.0	41.0	33.0	41.0
19	37.6175870082139	39.0	36.0	41.0	33.0	41.0
20	37.4460504558109	39.0	35.0	41.0	32.0	41.0
21	37.3732667631674	39.0	35.0	41.0	32.0	41.0
22	37.280560037693306	39.0	35.0	41.0	32.0	41.0
23	37.20765530232172	39.0	35.0	41.0	32.0	41.0
24	37.04924238256105	39.0	35.0	41.0	32.0	41.0
25	36.938920438914934	39.0	35.0	41.0	31.0	41.0
26	36.84060073205842	39.0	35.0	41.0	31.0	41.0
27	36.72606830211065	39.0	35.0	41.0	31.0	41.0
28	36.713662923011825	39.0	35.0	41.0	31.0	41.0
29	36.67426601590361	39.0	35.0	41.0	31.0	41.0
30	36.594777956618685	38.0	35.0	41.0	31.0	41.0
31	36.49055016420797	38.0	35.0	41.0	30.0	41.0
32	36.37587668446962	39.0	35.0	41.0	30.0	41.0
33	36.183004600624024	39.0	35.0	41.0	30.0	41.0
34	35.96751253192377	39.0	35.0	41.0	29.0	41.0
35	35.811192350899866	39.0	35.0	41.0	27.0	41.0
36	35.673563768010105	38.0	35.0	41.0	27.0	41.0
37	35.6272324130418	38.0	35.0	41.0	27.0	41.0
38	35.52522740527406	38.0	35.0	41.0	26.0	41.0
39	35.43713931017649	38.0	35.0	41.0	26.0	41.0
40	35.31992693420778	38.0	35.0	40.0	26.0	41.0
41	35.21893828521468	38.0	34.0	40.0	25.0	41.0
42	35.15162752451615	38.0	34.0	40.0	25.0	41.0
43	35.06578622253655	38.0	34.0	40.0	24.0	41.0
44	34.939937798042706	38.0	34.0	40.0	24.0	41.0
45	34.81939224546265	38.0	34.0	40.0	24.0	41.0
46	34.75533463312549	37.0	34.0	40.0	24.0	41.0
47	34.69435810841227	37.0	34.0	40.0	23.0	41.0
48	34.612505214340665	37.0	34.0	40.0	23.0	41.0
49	34.52345778059655	37.0	34.0	40.0	24.0	41.0
50	34.4248769815745	37.0	34.0	40.0	24.0	41.0
51	34.06892933205422	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	4.0
14	14.0
15	23.0
16	78.0
17	188.0
18	417.0
19	810.0
20	1455.0
21	2229.0
22	3554.0
23	5318.0
24	7796.0
25	11791.0
26	16258.0
27	18772.0
28	19222.0
29	20368.0
30	23060.0
31	27632.0
32	33527.0
33	42642.0
34	65658.0
35	90172.0
36	81409.0
37	107015.0
38	156467.0
39	263570.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.668284904571316	23.213594398822785	26.79335805539355	16.32476264121235
2	34.0085050022658	24.289374149074625	28.432636720762428	13.269484127897147
3	29.26683119141057	24.536261300238984	32.67683492272772	13.520072585622724
4	24.80585646733297	27.89854818751019	32.30120232442052	14.994393020736322
5	22.38420162317298	31.80272636240593	31.174404564811383	14.638667449609713
6	20.978605447723044	40.44287633534638	28.25597435894871	10.322543857981868
7	86.62677925307634	3.1546135785932434	8.676462791365353	1.5421443769650687
8	84.22263058859778	5.421813900306809	8.191491596533576	2.16406391456184
9	81.53058029484409	4.505390402812193	10.886743020286161	3.0772862820575666
10	37.28186049675536	26.914400783476566	20.985007707720825	14.818731012047254
11	30.702437960600093	25.13877398721749	25.65525630547583	18.503531746706585
12	27.214106579622605	22.48023552313967	30.31790221948348	19.987755677754247
13	24.05229045853186	25.468990553665442	31.233125293228508	19.245593694574183
14	21.851613619607722	26.98012398376627	31.640369050274746	19.527893346351263
15	21.148265337664196	23.919743669515338	35.66919122450225	19.262799768318217
16	23.567519334325016	21.895829227717385	34.06612534224581	20.470526095711786
17	23.032230377323195	22.974910143280578	30.39322880976985	23.59963066962638
18	24.327487603123902	23.14707091603336	31.960782156101104	20.564659324741633
19	26.24696517870808	25.77449839793447	27.64725948259736	20.331276940760088
20	27.62485157260513	24.378705683106137	27.761699880057662	20.234742864231073
21	26.181442049043312	24.814259433580055	28.99183411744346	20.012464399933176
22	24.679511867689293	22.690809855879127	29.037950396489958	23.59172787994162
23	23.387655842512405	25.872432968837998	28.160140529606952	22.57977065904264
24	22.867272147067915	24.616489620836155	32.16925574727879	20.34698248481714
25	23.469484728109023	25.35154909683118	29.688680104076738	21.490286070983057
26	22.410911051601214	26.15763364467657	28.47024999824938	22.96120530547283
27	22.316477716633973	25.09015682535935	29.69268151657535	22.900683941431325
28	21.288814951677942	26.339897983988347	30.97623461081762	21.39505245351609
29	22.4778346756405	24.479841384008555	30.352314366971537	22.690009573379402
30	23.09515258886387	25.023933448507325	30.638315325309833	21.242598637318974
31	25.021332530383226	25.806209591985972	27.66736658040288	21.50509129722792
32	25.25171385499081	25.087555907235252	27.51081131639469	22.149918921379246
33	24.212346958476342	25.934654933191414	27.06865523529806	22.78434287303418
34	22.028476052046372	26.9777231362671	28.361711684224534	22.63208912746199
35	22.469231638768488	26.616395587642437	28.474251410747993	22.440121362841083
36	25.35695100370431	27.63975683416246	25.981571494737643	21.02172066739559
37	23.074245208558622	27.898448152197723	27.24701819742369	21.780288441819962
38	23.764889005819054	27.865736605021574	27.1630885702653	21.20628581889407
39	23.055038428565283	26.911599794727536	26.667213526374812	23.366148250332365
40	24.360599291549917	25.385961244319244	28.25187291113763	22.001566552993207
41	22.025875133922273	25.149477765651273	28.696930016295752	24.127717084130698
42	23.17428052102392	26.277976125572327	26.79505865570546	23.752684697698285
43	22.730423839615383	25.289627238415164	28.564883403841556	23.4150655181279
44	22.819755373646895	26.48905063487411	28.121827004932744	22.569366986546253
45	22.012370366739457	26.989327232513077	27.170491183387735	23.82781121735973
46	23.29092169535846	26.674916245434638	27.73379002787984	22.300372031327058
47	22.692010279628708	26.349101232735155	28.347906811104316	22.610981676531814
48	22.953102445163143	25.976269623176982	28.232566095831828	22.838061835828046
49	22.634590010273627	25.491098357720276	28.637609076003827	23.23670255600227
50	22.278064156647297	26.235461117774573	29.170397150194017	22.31607757538411
51	21.687855813102026	26.739238951349826	28.486355683556297	23.08654955199185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	609.0
1	884.0
2	1159.0
3	7883.5
4	14608.0
5	10125.0
6	5642.0
7	5710.5
8	5779.0
9	6043.0
10	6307.0
11	6318.5
12	6330.0
13	6291.5
14	6253.0
15	6095.5
16	5938.0
17	5668.0
18	5398.0
19	5267.5
20	5137.0
21	4941.5
22	4746.0
23	4894.5
24	5043.0
25	5455.5
26	6847.5
27	7827.0
28	8685.5
29	9544.0
30	11029.0
31	12514.0
32	14903.5
33	17293.0
34	20614.5
35	23936.0
36	26367.0
37	28798.0
38	32102.0
39	35406.0
40	40556.5
41	45707.0
42	49957.5
43	54208.0
44	57950.5
45	61693.0
46	67057.5
47	72422.0
48	75944.5
49	79467.0
50	77538.5
51	75610.0
52	72883.5
53	70157.0
54	65945.5
55	61734.0
56	58316.5
57	54899.0
58	52201.5
59	49504.0
60	45999.5
61	42495.0
62	38221.5
63	33948.0
64	29997.5
65	26047.0
66	22815.0
67	19583.0
68	16294.5
69	13006.0
70	11611.5
71	10217.0
72	8140.5
73	6064.0
74	4992.0
75	3171.0
76	2422.0
77	1857.0
78	1292.0
79	976.5
80	661.0
81	479.5
82	298.0
83	190.5
84	83.0
85	61.0
86	39.0
87	27.0
88	15.0
89	13.5
90	12.0
91	7.5
92	3.0
93	2.0
94	1.0
95	2.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	999647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.083605560968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.02085670763924	24.206468666252047
2	7.500467288787077	5.112859371879237
3	3.904381012867402	3.9922614720691576
4	2.7483174839640188	3.7469027631896648
5	2.0356526852403514	3.469119159142874
6	1.6567793353597322	3.3881408020778303
7	1.3580337892990475	3.2400681609054827
8	1.1388503423829133	3.1052900690202065
9	1.0245620034327965	3.1428690477982726
>10	7.552682615766814	43.45554846918169
>50	0.041411875684088696	0.9251213983399356
>100	0.01710461659790722	1.210567402649275
>500	6.001619857573954E-4	0.12590473745271674
>1k	0.0	0.0
>5k	3.000809928786977E-4	0.8788784800415881
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8593	0.8596034400143251	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03141108811410428	0.0
2	0.0	0.0	0.0	0.10173591277721035	0.0
3	0.0	0.0	0.0	0.17096034900319812	0.0
4	0.0	0.0	0.0	0.29900554895878245	0.0
5	0.0	0.0	0.0	0.5014770213885501	0.0
6	0.0	0.0	0.0	1.044868838700061	0.0
7	0.0	0.0	0.0	1.323367148603457	0.0
8	0.0	0.0	0.0	1.9905026474345444	0.0
9	0.0	0.0	0.0	2.344227512311846	0.0
10	0.0	0.0	0.0	2.8301990602682747	0.0
11	0.0	0.0	0.0	3.1541134020309167	0.0
12	0.0	0.0	0.0	3.4278100169359784	0.0
13	0.0	0.0	0.0	3.5946689181280993	0.0
14	0.0	0.0	0.0	3.694204054031073	0.0
15	0.0	0.0	0.0	3.77813368118946	0.0
16	0.0	0.0	0.0	3.8939745730242774	0.0
17	0.0	0.0	0.0	4.018318466418646	0.0
18	0.0	0.0	0.0	4.197181605106603	0.0
19	0.0	0.0	0.0	4.304319424756939	0.0
20	0.0	0.0	0.0	4.412957774094255	0.0
21	0.0	0.0	0.0	4.539002267800534	0.0
22	0.0	0.0	0.0	4.685554000562198	0.0
23	0.0	0.0	0.0	4.854713713941021	0.0
24	0.0	0.0	0.0	4.998964634515984	0.0
25	0.0	0.0	0.0	5.119407150724205	0.0
26	0.0	0.0	0.0	5.235948289746281	0.0
27	0.0	0.0	0.0	5.3690952906375955	0.0
28	0.0	0.0	0.0	5.502342326841375	0.0
29	0.0	0.0	0.0	5.647793671165922	0.0
30	0.0	0.0	0.0	5.833159105164123	0.0
31	0.0	0.0	0.0	5.98051112042551	0.0
32	0.0	0.0	0.0	6.139967408495199	0.0
33	0.0	0.0	0.0	6.305125709375409	0.0
34	0.0	0.0	0.0	6.462581291195792	0.0
35	0.0	0.0	0.0	6.6353422758233656	0.0
36	0.0	0.0	0.0	6.801400894515764	0.0
37	0.0	0.0	0.0	6.97666276195497	0.0
38	0.0	0.0	0.0	7.182735505633489	0.0
39	0.0	0.0	0.0	7.4594331799125095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTT	30	2.1652158E-6	45.000004	26
CGTTTTT	5965	0.0	42.170998	1
GTTAGCG	45	1.9279469E-8	40.0	1
CGTTAGG	90	0.0	40.0	2
CGTAAGG	175	0.0	39.857143	2
TAGGGCG	465	0.0	39.19355	5
ATACTAT	900	0.0	39.0	45
TATACTA	925	0.0	37.945946	44
GTTGATC	560	0.0	37.76786	16
ATTATAC	935	0.0	37.540104	42
CATCGAA	30	1.1398153E-4	37.500004	24
ATAGTCG	30	1.1398153E-4	37.500004	1
TACGGGT	220	0.0	36.81818	4
AGGGCGA	1235	0.0	36.61943	6
GTAGGGA	960	0.0	36.562504	4
AATGCGG	105	0.0	36.42857	2
CGAATAT	105	0.0	36.42857	14
ACTAATC	1045	0.0	36.387558	10
TATGGGT	365	0.0	36.369865	4
TAAGGGA	1015	0.0	36.35468	4
>>END_MODULE