FastQCFastQC Report
Thu 26 May 2016
SRR1547115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547115_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences917625
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.9079961858057486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG302.1650212E-645.0000042
TAATGCG302.1650212E-645.0000041
CGTTTTT51300.040.9210551
TCACGAC900.040.025
ATACCGG451.927583E-840.02
GCGAGAC7900.038.44936421
AGGGCGA11100.038.108116
TCAAGCG8000.037.9687517
CACGACC7950.037.9245327
ACGACCA7950.037.6415128
AAGGGCG4250.037.5882345
CGAGACA8150.037.5460122
GCGAATA301.1397424E-437.50000425
ACGGGAT2400.037.5000045
GCGATGT2400.037.5000049
TTACGAG601.546141E-1037.5000041
CGGTCTA900.037.531
ACACGAC8050.037.4534226
GACACGA8100.037.2222225
GGGCGAT22650.037.1523177
Produced by FastQC (version 0.11.5)