##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547115_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	917625
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.439605503337418	31.0	31.0	33.0	30.0	34.0
2	31.804763383735185	31.0	31.0	34.0	30.0	34.0
3	31.88678272714889	31.0	31.0	34.0	30.0	34.0
4	35.66621768151478	37.0	35.0	37.0	33.0	37.0
5	35.610735322163194	37.0	35.0	37.0	33.0	37.0
6	35.64846533169868	37.0	35.0	37.0	33.0	37.0
7	36.012541070698816	37.0	35.0	37.0	35.0	37.0
8	34.69538863914998	37.0	35.0	37.0	33.0	37.0
9	36.990644326386054	39.0	37.0	39.0	33.0	39.0
10	36.8926129955047	39.0	37.0	39.0	32.0	39.0
11	36.8033401443945	39.0	37.0	39.0	32.0	39.0
12	36.53643917722381	39.0	35.0	39.0	32.0	39.0
13	36.49132815692685	39.0	35.0	39.0	32.0	39.0
14	37.56627162511919	40.0	36.0	41.0	32.0	41.0
15	37.69081378558779	40.0	36.0	41.0	33.0	41.0
16	37.71172101893475	40.0	36.0	41.0	33.0	41.0
17	37.58331235526495	40.0	36.0	41.0	32.0	41.0
18	37.50130826862825	39.0	36.0	41.0	32.0	41.0
19	37.41169540934477	39.0	36.0	41.0	32.0	41.0
20	37.28822340280616	39.0	35.0	41.0	32.0	41.0
21	37.185069064160196	39.0	35.0	41.0	32.0	41.0
22	37.11157825909277	39.0	35.0	41.0	32.0	41.0
23	37.030327203378285	39.0	35.0	41.0	32.0	41.0
24	36.888186350633426	39.0	35.0	41.0	31.0	41.0
25	36.78599863778777	38.0	35.0	41.0	31.0	41.0
26	36.672396676202155	38.0	35.0	41.0	31.0	41.0
27	36.56709440130772	38.0	35.0	41.0	31.0	41.0
28	36.5877880397766	38.0	35.0	41.0	31.0	41.0
29	36.52471979294374	38.0	35.0	41.0	31.0	41.0
30	36.42558670480861	38.0	35.0	41.0	30.0	41.0
31	36.31726685737638	38.0	35.0	41.0	30.0	41.0
32	36.21184688734505	38.0	35.0	41.0	30.0	41.0
33	36.01975534668301	38.0	35.0	41.0	30.0	41.0
34	35.7799449666258	38.0	35.0	41.0	28.0	41.0
35	35.623055169595425	38.0	35.0	41.0	27.0	41.0
36	35.52130227489443	38.0	35.0	41.0	27.0	41.0
37	35.496601552921945	38.0	35.0	41.0	27.0	41.0
38	35.392436997684236	38.0	34.0	40.0	27.0	41.0
39	35.30211251873042	38.0	34.0	40.0	26.0	41.0
40	35.16141888026154	38.0	34.0	40.0	26.0	41.0
41	35.05986759297099	38.0	34.0	40.0	25.0	41.0
42	35.00203895926985	38.0	34.0	40.0	25.0	41.0
43	34.897047268764474	37.0	34.0	40.0	24.0	41.0
44	34.7685971938428	37.0	34.0	40.0	24.0	41.0
45	34.638549516414656	37.0	34.0	40.0	24.0	41.0
46	34.53918103800572	37.0	34.0	40.0	23.0	41.0
47	34.46920474049857	36.0	34.0	40.0	23.0	41.0
48	34.39234654679199	36.0	34.0	40.0	23.0	41.0
49	34.30992289878763	36.0	33.0	40.0	24.0	41.0
50	34.19720365072878	36.0	33.0	40.0	24.0	41.0
51	33.85151668709985	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	1.0
12	3.0
13	4.0
14	12.0
15	25.0
16	73.0
17	201.0
18	409.0
19	824.0
20	1364.0
21	2207.0
22	3364.0
23	4971.0
24	7100.0
25	10864.0
26	14487.0
27	17115.0
28	18425.0
29	19598.0
30	22451.0
31	26492.0
32	32360.0
33	41412.0
34	66805.0
35	89824.0
36	75611.0
37	97317.0
38	139201.0
39	224948.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.43092221768151	22.642909685328974	25.68729055986923	16.238877537120285
2	34.17027652908323	24.788884348181448	27.23966762021523	13.801171502520093
3	30.060318757662447	25.236561776324752	31.357253780138944	13.345865685873859
4	25.815992371611497	28.043808745402533	30.917858602370252	15.22234028061572
5	23.1630568042501	31.364010352812972	30.444462607274215	15.028470235662716
6	21.87745538754938	40.141888026154476	27.909331153793758	10.071325432502384
7	86.31488897970304	3.537501702765291	8.507832720337829	1.6397765971938427
8	83.7691867592971	5.767606593107206	8.192235390273805	2.2709712573218908
9	80.7228442991418	5.153412341642828	10.628851655087862	3.494891704127503
10	41.17629750715162	29.110693366026425	16.62029696226672	13.092712164555238
11	33.32821141533851	25.467157063070427	25.09805203650729	16.106579485083778
12	29.322762566407846	22.72180901784498	29.175207737365483	18.780220678381692
13	24.937637923988557	25.61830813240703	30.845061980656585	18.598991962947828
14	21.65263588067021	26.74447622939654	32.09426508650048	19.50862280343277
15	21.479689415610952	22.843100395041546	36.08947009944149	19.58774008990601
16	25.009998637787767	22.27227898106525	33.867538482495576	18.85018389865141
17	24.562103255687237	22.369704399945512	30.602697180220677	22.465495164146574
18	25.98348998774009	22.00016346546792	31.23432774826318	20.78201879852881
19	27.271924805884755	25.45113744721428	27.509821550197522	19.767116196703448
20	29.30369159515052	24.884130227489443	26.54809971393543	19.264078463424603
21	27.50012259910094	25.19297098487944	28.654733687508514	18.6521727285111
22	26.39367933524043	22.410461789946872	28.814058030241114	22.381800844571583
23	24.628143304726876	25.69295736275712	28.524070290151204	21.1548290423648
24	24.07868137855878	24.005121917994824	31.818335376651678	20.097861326794714
25	24.966516823321072	24.625745811197387	29.176188530173004	21.23154883530854
26	22.483585342596378	25.992099169050537	28.586623075875224	22.937692412477865
27	22.235117831358124	24.24781364936657	30.172782999591334	23.344285519683964
28	21.51423511783136	26.33123552649503	30.463424601552923	21.69110475412069
29	23.229968669118648	24.975997820460428	29.772946465059256	22.021087045361668
30	24.435689960495846	24.646778368069747	30.35259501430323	20.56493665713118
31	26.354011715025198	24.97512600463152	27.72428824410843	20.946574036234843
32	27.141479362484677	24.871597874948918	26.997956681651004	20.988966080915404
33	26.217572537801388	25.134668301321344	26.48750851382645	22.160250647050812
34	23.3593243427326	26.910775098760386	29.148508377605232	20.581392180901783
35	22.800272442446534	27.706524996594467	28.689061435771695	20.804141125187304
36	25.10317395450211	28.831385369840625	26.0114970712437	20.05394360441357
37	23.70957635199564	28.82441084320937	25.979348862552786	21.486663942242203
38	23.816809698951097	28.770031330881352	26.433782863370116	20.97937610679744
39	23.7461926168097	27.266149026018255	25.71268219588612	23.274976161285927
40	25.009671706851925	25.779267129818827	27.032066475956956	22.178994687372292
41	21.467810924942107	25.879852881078875	27.895926985424328	24.756409208554693
42	23.49946873722926	26.55518321754529	26.157308268628256	23.788039776597195
43	23.045252690369157	25.173573082686286	27.850156654406753	23.9310175725378
44	23.11990192071925	25.93194387685601	27.67786405121918	23.270290151205558
45	22.78414384961177	26.088107887208828	26.843318348998775	24.28442991418063
46	23.553957226535893	26.551478000272443	27.34232393406893	22.552240839122735
47	22.511265495164146	26.46277073968124	28.637624301866232	22.388339463288382
48	23.218199155428415	25.645116469145897	28.881514780002725	22.255169595422966
49	23.787494891704128	25.459092766653047	28.061898923852336	22.691513417790492
50	22.003105843890477	26.730636153112652	29.088897970303773	22.177360032693095
51	22.329709848794444	26.476174908050677	27.68156926849203	23.512545974662853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	642.0
1	1148.0
2	1654.0
3	8118.5
4	14583.0
5	9821.5
6	5060.0
7	4997.0
8	4934.0
9	5103.5
10	5273.0
11	5263.0
12	5253.0
13	5288.5
14	5324.0
15	5264.0
16	5204.0
17	4997.5
18	4791.0
19	4768.0
20	4745.0
21	4609.0
22	4473.0
23	4662.5
24	4852.0
25	5425.5
26	6735.5
27	7472.0
28	8863.5
29	10255.0
30	11616.5
31	12978.0
32	15489.0
33	18000.0
34	19557.5
35	21115.0
36	23263.0
37	25411.0
38	28354.0
39	31297.0
40	35588.5
41	39880.0
42	43699.0
43	47518.0
44	50163.0
45	52808.0
46	56109.5
47	59411.0
48	62493.5
49	65576.0
50	65609.5
51	65643.0
52	63196.5
53	60750.0
54	58646.0
55	56542.0
56	53923.5
57	51305.0
58	49303.5
59	47302.0
60	45503.0
61	43704.0
62	39895.5
63	36087.0
64	32507.0
65	28927.0
66	24719.5
67	20512.0
68	17549.0
69	14586.0
70	12464.0
71	10342.0
72	8375.0
73	6408.0
74	5793.0
75	4213.5
76	3249.0
77	2410.0
78	1571.0
79	1045.5
80	520.0
81	412.0
82	304.0
83	202.5
84	101.0
85	77.5
86	54.0
87	38.5
88	23.0
89	12.5
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	917625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.15546125741445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8883327437434	22.79447037144146
2	6.948468818608785	4.468624397902544
3	3.566990849449006	3.4409470819502816
4	2.5143754190434566	3.234036054945884
5	2.0194389279247384	3.246799510429926
6	1.6434193365266685	3.1706944083221438
7	1.4149313927883609	3.184844010789393
8	1.2258928902663546	3.153532107095968
9	1.049697879482091	3.0378167546108834
>10	8.649148128276472	46.76349296514547
>50	0.056695156411174286	1.1959486189253976
>100	0.021912812634013807	1.3189751645393422
>500	3.478224227613825E-4	0.05793514247693979
>1k	0.0	0.0
>5k	3.478224227613825E-4	0.9318834114244448
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8332	0.9079961858057486	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026699359760250646	0.0
2	0.0	0.0	0.0	0.08521999727557554	0.0
3	0.0	0.0	0.0	0.1502792535076965	0.0
4	0.0	0.0	0.0	0.25446124506198065	0.0
5	0.0	0.0	0.0	0.4328565590519003	0.0
6	0.0	0.0	0.0	0.875630023157608	0.0
7	0.0	0.0	0.0	1.1152703991281843	0.0
8	0.0	0.0	0.0	1.6632066475956955	0.0
9	0.0	0.0	0.0	2.0180356899604956	0.0
10	0.0	0.0	0.0	2.4503473641193296	0.0
11	0.0	0.0	0.0	2.743386459610407	0.0
12	0.0	0.0	0.0	2.9833537665168235	0.0
13	0.0	0.0	0.0	3.1285111020296963	0.0
14	0.0	0.0	0.0	3.2286609453752892	0.0
15	0.0	0.0	0.0	3.2990600735594606	0.0
16	0.0	0.0	0.0	3.3977932161830813	0.0
17	0.0	0.0	0.0	3.49946873722926	0.0
18	0.0	0.0	0.0	3.6419016482768014	0.0
19	0.0	0.0	0.0	3.7288652772101893	0.0
20	0.0	0.0	0.0	3.82727148889797	0.0
21	0.0	0.0	0.0	3.9480179812014713	0.0
22	0.0	0.0	0.0	4.078899332516006	0.0
23	0.0	0.0	0.0	4.215229532761204	0.0
24	0.0	0.0	0.0	4.337937610679744	0.0
25	0.0	0.0	0.0	4.446369704399945	0.0
26	0.0	0.0	0.0	4.545865685873859	0.0
27	0.0	0.0	0.0	4.654406756572674	0.0
28	0.0	0.0	0.0	4.767415883394633	0.0
29	0.0	0.0	0.0	4.89437406347909	0.0
30	0.0	0.0	0.0	5.0554420378695	0.0
31	0.0	0.0	0.0	5.179021931616946	0.0
32	0.0	0.0	0.0	5.347064432638605	0.0
33	1.0897697861326794E-4	0.0	0.0	5.490042228579212	0.0
34	1.0897697861326794E-4	0.0	0.0	5.63334695545566	0.0
35	1.0897697861326794E-4	0.0	0.0	5.785369840621168	0.0
36	1.0897697861326794E-4	0.0	0.0	5.927584797711483	0.0
37	1.0897697861326794E-4	0.0	0.0	6.074812695818008	0.0
38	1.0897697861326794E-4	0.0	0.0	6.268682740771012	0.0
39	1.0897697861326794E-4	0.0	0.0	6.567715570085819	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	30	2.1650212E-6	45.000004	2
TAATGCG	30	2.1650212E-6	45.000004	1
CGTTTTT	5130	0.0	40.921055	1
TCACGAC	90	0.0	40.0	25
ATACCGG	45	1.927583E-8	40.0	2
GCGAGAC	790	0.0	38.449364	21
AGGGCGA	1110	0.0	38.10811	6
TCAAGCG	800	0.0	37.96875	17
CACGACC	795	0.0	37.92453	27
ACGACCA	795	0.0	37.64151	28
AAGGGCG	425	0.0	37.588234	5
CGAGACA	815	0.0	37.54601	22
GCGAATA	30	1.1397424E-4	37.500004	25
ACGGGAT	240	0.0	37.500004	5
GCGATGT	240	0.0	37.500004	9
TTACGAG	60	1.546141E-10	37.500004	1
CGGTCTA	90	0.0	37.5	31
ACACGAC	805	0.0	37.45342	26
GACACGA	810	0.0	37.22222	25
GGGCGAT	2265	0.0	37.152317	7
>>END_MODULE