FastQCFastQC Report
Thu 26 May 2016
SRR1547114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547114_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1532438
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251181.6390875193645682No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT27020.17632034705482377No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC23050.15041391560376344TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT19930.13005420121401323TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC16850.1099555088036188RNA PCR Primer, Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA207.034023E-445.00000435
CGACGGT850.045.028
ACGTTAG551.8189894E-1245.01
CTCGTAG302.165978E-644.9999961
CGTTTTT130600.043.22551
CGGTCTA900.042.531
ACGGGTA1900.040.2631575
GCGATAC1600.039.3750049
CGTTAGG2250.039.0000042
CCCGCGA356.2496547E-638.57142629
GCGATAT1300.038.076929
GGGCGAT30100.037.749177
CATACGA3100.037.74193618
TAGGGAC12300.037.6829265
AGGGCGA16200.037.6388856
ACGGGAT4250.037.5882345
GGCGATT8500.037.5882348
GTTTTTT148650.037.2805252
TCACGAC1100.036.8181825
TAGGGCG5950.036.680675
Produced by FastQC (version 0.11.5)