##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547114_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1532438
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34880301845817	31.0	31.0	33.0	30.0	34.0
2	31.695675779379002	31.0	31.0	34.0	30.0	34.0
3	31.700217561819795	31.0	31.0	34.0	30.0	34.0
4	35.52644283161864	37.0	35.0	37.0	33.0	37.0
5	35.481261884656995	37.0	35.0	37.0	33.0	37.0
6	35.53913698302966	37.0	35.0	37.0	33.0	37.0
7	35.97020956149613	37.0	35.0	37.0	35.0	37.0
8	34.66526280345437	37.0	35.0	37.0	33.0	37.0
9	36.94297779094489	39.0	37.0	39.0	33.0	39.0
10	36.79804207413285	39.0	37.0	39.0	32.0	39.0
11	36.71166533327939	39.0	35.0	39.0	32.0	39.0
12	36.51874659855733	39.0	35.0	39.0	32.0	39.0
13	36.46767503807658	39.0	35.0	39.0	32.0	39.0
14	37.59344521605442	40.0	36.0	41.0	32.0	41.0
15	37.73701252513968	40.0	36.0	41.0	33.0	41.0
16	37.7723150952926	40.0	36.0	41.0	33.0	41.0
17	37.68951174533652	40.0	36.0	41.0	33.0	41.0
18	37.55193228045768	39.0	36.0	41.0	32.0	41.0
19	37.415969194186	39.0	36.0	41.0	32.0	41.0
20	37.197458559498	39.0	35.0	41.0	32.0	41.0
21	37.10052021680485	39.0	35.0	41.0	32.0	41.0
22	37.00323275721432	39.0	35.0	41.0	32.0	41.0
23	36.918632270930374	38.0	35.0	41.0	32.0	41.0
24	36.76674619136304	38.0	35.0	41.0	31.0	41.0
25	36.66545139183445	38.0	35.0	41.0	31.0	41.0
26	36.53620570620149	38.0	35.0	40.0	31.0	41.0
27	36.39360287332995	38.0	35.0	40.0	31.0	41.0
28	36.382838326901314	38.0	35.0	40.0	31.0	41.0
29	36.29618686041458	38.0	35.0	40.0	30.0	41.0
30	36.23219144918098	38.0	35.0	40.0	30.0	41.0
31	36.09469420622563	38.0	35.0	40.0	30.0	41.0
32	35.85692145457108	38.0	35.0	40.0	29.0	41.0
33	35.590694696946954	38.0	35.0	40.0	27.0	41.0
34	35.31067945326336	38.0	35.0	40.0	25.0	41.0
35	35.11663701892018	38.0	34.0	40.0	24.0	41.0
36	34.98081031663271	38.0	34.0	40.0	24.0	41.0
37	34.93877142174757	38.0	34.0	40.0	23.0	41.0
38	34.85187850992993	38.0	34.0	40.0	23.0	41.0
39	34.76169606861746	38.0	34.0	40.0	23.0	41.0
40	34.6333228489505	38.0	34.0	40.0	23.0	41.0
41	34.53465784586391	38.0	34.0	40.0	22.0	41.0
42	34.4521102974476	38.0	34.0	40.0	22.0	41.0
43	34.372151434511544	37.0	34.0	40.0	22.0	41.0
44	34.25236192263569	37.0	33.0	40.0	21.0	41.0
45	34.15888799416355	37.0	33.0	40.0	21.0	41.0
46	34.08303631207266	37.0	33.0	40.0	20.0	41.0
47	34.01397837954945	37.0	33.0	40.0	20.0	41.0
48	33.922586753917614	37.0	33.0	40.0	20.0	41.0
49	33.864168729827895	37.0	33.0	40.0	20.0	41.0
50	33.76859814230657	36.0	33.0	40.0	20.0	41.0
51	33.421990971249734	36.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	5.0
13	11.0
14	13.0
15	46.0
16	106.0
17	338.0
18	771.0
19	1511.0
20	2695.0
21	4170.0
22	6362.0
23	9694.0
24	14696.0
25	23000.0
26	31711.0
27	35023.0
28	34072.0
29	34821.0
30	38480.0
31	45162.0
32	54590.0
33	68809.0
34	104622.0
35	156394.0
36	126629.0
37	153019.0
38	213985.0
39	371433.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.76037138207223	23.681088566062705	26.527990039401267	18.0305500124638
2	35.47216918400614	24.83017257468165	27.071633566904502	12.62602467440771
3	27.2841054581001	23.929777256893917	35.82363527920869	12.962482005797298
4	23.829414305831623	26.90183876933357	35.1150258607526	14.1537210640822
5	20.807562850829857	31.0783209500156	33.87282226099849	14.241293938156064
6	19.816658161700506	39.05358650725184	30.806858091485594	10.322897239562058
7	81.777076788751	3.407707195984438	12.670006877929154	2.145209137335409
8	78.87268522445933	5.8741691344119635	11.819988802157086	3.433156838971626
9	75.40337684134693	5.612559855602641	15.182343429228458	3.801719873821975
10	37.20731279177363	28.994190955849437	20.976835604442073	12.82166064793486
11	29.27739980345045	24.94123742689753	29.728184761797866	16.05317800785415
12	26.918283154032984	22.04787404123364	32.72302044193631	18.31082236279706
13	20.950015596063267	27.53527385773519	34.744439905562245	16.770270640639296
14	18.08171032041753	30.60639321134036	33.35528093143083	17.956615536811277
15	17.0859767246701	25.86225348105437	40.27047097500845	16.781298819267075
16	18.43415524804266	23.772185236857872	39.51859716347415	18.27506235162532
17	18.90464736583144	24.756368610018807	32.77881389002361	23.560170134126142
18	20.16218600687271	25.710142922584794	34.49457661582394	19.63309445471856
19	23.481602518340054	27.925893249841106	29.724921987055918	18.86758224476292
20	24.70807954383799	25.6593741476001	29.84342596568344	19.789120342878473
21	21.913056188896384	28.548300159614943	30.957141496099677	18.581502155388996
22	21.63669916825346	25.690435763143437	30.288859973454063	22.384005095149035
23	19.711074771051095	29.014681181228863	29.77373309719545	21.50051095052459
24	19.66663577906578	26.128691666481775	35.51497678862049	18.689695765831964
25	19.760538436139015	27.13617125130022	32.674470353776144	20.42881995878463
26	18.86588560189711	29.86567808942352	30.834656932286986	20.433779376392387
27	19.83975860687349	28.33706812282128	30.931561342122816	20.891611928182414
28	18.65667648544346	27.748920347837892	33.83667071685771	19.75773244986094
29	18.330660033228096	25.204021304613956	33.907081395788936	22.558237266369012
30	19.521964347007838	27.20057842470625	32.40718384691583	20.870273381370076
31	21.98992716181666	26.183245260167133	30.266999382683018	21.55982819533319
32	22.293430468312582	26.161319413901246	29.91135693581078	21.633893181975388
33	21.118179006263222	26.444724027986776	31.144685788266802	21.2924111774832
34	18.138939389391282	26.560944064294933	31.448450116742084	23.8516664295717
35	18.687738100986795	25.988261841588372	32.199149329369284	23.124850728055556
36	21.38252901585578	26.59076582543633	30.611091606968767	21.415613551739124
37	19.45109687961275	28.435016620574537	30.375649781589857	21.738236718222858
38	19.12749488070643	28.890238952571	30.642675266470814	21.339590900251757
39	19.67146468568386	28.744327666111126	28.431231801873874	23.152975846331138
40	21.433493557324994	26.799844430900304	29.790830036843253	21.97583197493145
41	18.469523726245367	26.510958355248306	29.67284810217444	25.346669816331886
42	20.411135719683276	28.135885432232822	27.587869786575382	23.865109061508523
43	20.25093347985367	27.012512088580422	29.960168045950308	22.776386385615602
44	20.559069926483158	28.778130012437696	28.616492151721634	22.046307909357505
45	19.34479567852011	29.305002877767322	27.83075073836592	23.519450705346646
46	21.064930522474647	28.960910653481577	28.00211166781299	21.97204715623079
47	20.816698620107307	27.15535636678286	29.141668374185446	22.88627663892438
48	20.47260639582156	25.7062928483893	30.75648084946993	23.064619906319212
49	20.250672457874316	25.723128766057744	30.26823923708496	23.75795953898298
50	18.49399453680997	27.748659325858533	31.144359510792608	22.612986626538888
51	18.557227111308908	28.124269954151487	29.253320525854882	24.065182408684723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1373.0
1	1711.5
2	2050.0
3	18870.0
4	35690.0
5	23544.0
6	11398.0
7	11804.5
8	12211.0
9	13373.5
10	14536.0
11	15681.5
12	16827.0
13	17210.5
14	17594.0
15	16932.0
16	16270.0
17	15827.0
18	15384.0
19	14360.0
20	13336.0
21	12655.5
22	11975.0
23	11681.0
24	11387.0
25	12042.0
26	13916.5
27	15136.0
28	16743.5
29	18351.0
30	20875.0
31	23399.0
32	25830.5
33	28262.0
34	33170.0
35	38078.0
36	41490.5
37	44903.0
38	50207.0
39	55511.0
40	66671.5
41	77832.0
42	87149.0
43	96466.0
44	106140.0
45	115814.0
46	126631.0
47	137448.0
48	140775.5
49	144103.0
50	133289.0
51	122475.0
52	107942.0
53	93409.0
54	83074.0
55	72739.0
56	67369.5
57	62000.0
58	57259.5
59	52519.0
60	46609.0
61	40699.0
62	36950.0
63	33201.0
64	27870.5
65	22540.0
66	19094.5
67	15649.0
68	13060.5
69	10472.0
70	8748.5
71	7025.0
72	5788.0
73	4551.0
74	3910.5
75	2537.5
76	1805.0
77	1478.5
78	1152.0
79	826.0
80	500.0
81	351.5
82	203.0
83	151.0
84	99.0
85	81.5
86	64.0
87	40.5
88	17.0
89	11.5
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1532438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.313653658889365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09751297095303	26.454973580470124
2	7.745294795738383	5.315387262139303
3	3.2255094217377227	3.3203703951297814
4	1.9414279174990425	2.6646994065904392
5	1.299169065534104	2.228961867954009
6	0.9654725901894022	1.9877335246145984
7	0.7903862384938074	1.8984727751100186
8	0.6400902524911252	1.7571068187529209
9	0.5366858563891259	1.6574087339784813
>10	5.343754098471668	39.40753483024507
>50	0.37626543260029505	8.072301536426385
>100	0.0341395361146467	1.9288791885114878
>500	0.0019508289943886896	0.458338662447651
>1k	0.0021459118938275588	1.1664307277859383
>5k	0.0	0.0
>10k+	1.95082899438869E-4	1.6814006898437415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25118	1.6390875193645682	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2702	0.17632034705482377	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	2305	0.15041391560376344	TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	1993	0.13005420121401323	TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1685	0.1099555088036188	RNA PCR Primer, Index 10 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.525549483894292E-5	0.0	0.0	0.13918997049146523	0.0
2	6.525549483894292E-5	0.0	0.0	0.4148291806911601	0.0
3	6.525549483894292E-5	0.0	0.0	0.6866835721901963	0.0
4	6.525549483894292E-5	0.0	0.0	1.0932253050368106	0.0
5	6.525549483894292E-5	0.0	0.0	1.8544959078279186	0.0
6	6.525549483894292E-5	0.0	0.0	3.2272104972599216	0.0
7	6.525549483894292E-5	0.0	0.0	3.9496540806218587	0.0
8	6.525549483894292E-5	0.0	0.0	5.416271327127101	0.0
9	6.525549483894292E-5	0.0	0.0	6.12129169336704	0.0
10	6.525549483894292E-5	0.0	0.0	7.045505266770989	0.0
11	6.525549483894292E-5	0.0	0.0	7.748045924207048	0.0
12	6.525549483894292E-5	0.0	0.0	8.336324210180118	0.0
13	6.525549483894292E-5	0.0	0.0	8.678458769620695	0.0
14	6.525549483894292E-5	0.0	0.0	8.865024229365234	0.0
15	6.525549483894292E-5	0.0	0.0	9.024443403256772	0.0
16	6.525549483894292E-5	0.0	0.0	9.295188451343545	0.0
17	6.525549483894292E-5	0.0	0.0	9.603455408962711	0.0
18	6.525549483894292E-5	0.0	0.0	10.030161089714559	0.0
19	6.525549483894292E-5	0.0	0.0	10.25738072274376	6.525549483894292E-5
20	6.525549483894292E-5	0.0	0.0	10.499739630575593	6.525549483894292E-5
21	6.525549483894292E-5	0.0	0.0	10.781773879269505	6.525549483894292E-5
22	6.525549483894292E-5	0.0	0.0	11.087169595115757	6.525549483894292E-5
23	1.3051098967788584E-4	0.0	0.0	11.432110140834409	6.525549483894292E-5
24	1.3051098967788584E-4	0.0	0.0	11.711403658745084	6.525549483894292E-5
25	1.3051098967788584E-4	0.0	0.0	11.943974242351077	6.525549483894292E-5
26	1.3051098967788584E-4	0.0	0.0	12.171781174833827	6.525549483894292E-5
27	1.3051098967788584E-4	0.0	0.0	12.402394093594651	6.525549483894292E-5
28	1.3051098967788584E-4	0.0	0.0	12.634834166210966	6.525549483894292E-5
29	1.9576648451682873E-4	0.0	0.0	12.889983151031233	6.525549483894292E-5
30	1.9576648451682873E-4	0.0	0.0	13.212802083999483	6.525549483894292E-5
31	1.9576648451682873E-4	0.0	0.0	13.46416625011909	6.525549483894292E-5
32	1.9576648451682873E-4	0.0	0.0	13.719184723949681	6.525549483894292E-5
33	1.9576648451682873E-4	0.0	0.0	13.96917852467767	6.525549483894292E-5
34	1.9576648451682873E-4	0.0	0.0	14.21558327318952	6.525549483894292E-5
35	1.9576648451682873E-4	0.0	0.0	14.491091972399536	6.525549483894292E-5
36	1.9576648451682873E-4	0.0	0.0	14.758835267723718	6.525549483894292E-5
37	1.9576648451682873E-4	0.0	0.0	15.013070675616241	6.525549483894292E-5
38	1.9576648451682873E-4	0.0	0.0	15.278334262136543	6.525549483894292E-5
39	1.9576648451682873E-4	0.0	0.0	15.55886763444916	6.525549483894292E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	20	7.034023E-4	45.000004	35
CGACGGT	85	0.0	45.0	28
ACGTTAG	55	1.8189894E-12	45.0	1
CTCGTAG	30	2.165978E-6	44.999996	1
CGTTTTT	13060	0.0	43.2255	1
CGGTCTA	90	0.0	42.5	31
ACGGGTA	190	0.0	40.263157	5
GCGATAC	160	0.0	39.375004	9
CGTTAGG	225	0.0	39.000004	2
CCCGCGA	35	6.2496547E-6	38.571426	29
GCGATAT	130	0.0	38.07692	9
GGGCGAT	3010	0.0	37.74917	7
CATACGA	310	0.0	37.741936	18
TAGGGAC	1230	0.0	37.682926	5
AGGGCGA	1620	0.0	37.638885	6
ACGGGAT	425	0.0	37.588234	5
GGCGATT	850	0.0	37.588234	8
GTTTTTT	14865	0.0	37.280525	2
TCACGAC	110	0.0	36.81818	25
TAGGGCG	595	0.0	36.68067	5
>>END_MODULE