FastQCFastQC Report
Thu 26 May 2016
SRR1547113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547113_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1464355
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301442.058517231135893No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT30730.20985348498144232No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC25050.17106507643296878No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG18110.12367219697409439No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT17560.11991627713225277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG302.1659125E-644.9999961
CGTTTTT203250.044.025831
GCGTAAG900.042.51
ACGGGTA1600.040.781255
TATACGG1100.038.8636322
GTTTTTT227750.038.4006582
GCGAGAC1650.038.18181621
GCGATAC950.037.8947379
AGGGTAC6850.037.4452556
AGGGCGA15950.037.3824466
GGGCGAT28950.037.072547
GGCGATT8200.036.768298
CCTCGTC2150.036.62790736
TAGGGAC10700.036.5887835
TGGGCGA7450.036.5436256
GCGATTG4200.036.428579
TTAGGGA13750.036.327274
GGCACCG6850.036.1313868
TAATACG250.002107296836.01
CGTAAGA250.002107296836.027
Produced by FastQC (version 0.11.5)