##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547113_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1464355
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.521987496201398	31.0	31.0	33.0	30.0	34.0
2	31.881637307893236	31.0	31.0	34.0	30.0	34.0
3	31.779351318498588	31.0	31.0	34.0	30.0	34.0
4	35.66371610709152	37.0	35.0	37.0	33.0	37.0
5	35.64921620781846	37.0	35.0	37.0	33.0	37.0
6	35.713551017342105	37.0	35.0	37.0	33.0	37.0
7	36.09715198841811	37.0	35.0	37.0	35.0	37.0
8	34.80351622386648	37.0	35.0	37.0	35.0	37.0
9	37.130253934326035	39.0	37.0	39.0	34.0	39.0
10	37.00915078652376	39.0	37.0	39.0	32.0	39.0
11	36.93288580979339	39.0	37.0	39.0	33.0	39.0
12	36.78388027493333	39.0	35.0	39.0	33.0	39.0
13	36.76605877673105	39.0	35.0	39.0	33.0	39.0
14	37.94112561503187	40.0	37.0	41.0	33.0	41.0
15	38.060834975125566	40.0	37.0	41.0	33.0	41.0
16	38.068375496378955	40.0	36.0	41.0	33.0	41.0
17	37.963059504013714	40.0	36.0	41.0	33.0	41.0
18	37.73048816714526	39.0	36.0	41.0	33.0	41.0
19	37.52057185586828	39.0	36.0	41.0	33.0	41.0
20	37.226459430944	39.0	35.0	41.0	33.0	41.0
21	37.162868976443555	39.0	35.0	41.0	33.0	41.0
22	37.07767378811832	38.0	35.0	41.0	32.0	41.0
23	37.00328677130887	38.0	35.0	41.0	32.0	41.0
24	36.845529260322806	38.0	35.0	41.0	32.0	41.0
25	36.76127714932513	38.0	35.0	41.0	32.0	41.0
26	36.66489683171089	38.0	35.0	40.0	32.0	41.0
27	36.549566191258265	38.0	35.0	40.0	31.0	41.0
28	36.523713170645095	38.0	35.0	40.0	31.0	41.0
29	36.45608544376193	38.0	35.0	40.0	31.0	41.0
30	36.317265963512945	38.0	35.0	40.0	31.0	41.0
31	36.089571859282756	38.0	35.0	40.0	30.0	41.0
32	35.698726743173616	38.0	35.0	40.0	29.0	41.0
33	35.1887779944071	38.0	35.0	41.0	24.0	41.0
34	34.66369971762312	38.0	34.0	41.0	21.0	41.0
35	34.302273697293344	38.0	34.0	41.0	18.0	41.0
36	34.09961792051791	38.0	34.0	40.0	18.0	41.0
37	34.0049154747312	38.0	34.0	40.0	16.0	41.0
38	33.88130200668554	38.0	33.0	40.0	15.0	41.0
39	33.775197954048025	38.0	33.0	40.0	15.0	41.0
40	33.667584704528615	38.0	33.0	40.0	15.0	41.0
41	33.571194143496626	37.0	33.0	40.0	15.0	41.0
42	33.49818657361091	37.0	33.0	40.0	15.0	41.0
43	33.423590591079346	37.0	33.0	40.0	13.0	41.0
44	33.312883146504774	37.0	33.0	40.0	12.0	41.0
45	33.22437455398452	37.0	33.0	40.0	12.0	41.0
46	33.17128974872896	37.0	33.0	40.0	11.0	41.0
47	33.11604016785547	37.0	33.0	40.0	11.0	41.0
48	33.025384554974714	36.0	33.0	40.0	10.0	41.0
49	32.95799242669981	36.0	33.0	40.0	10.0	41.0
50	32.87470251407616	36.0	32.0	40.0	10.0	41.0
51	32.54474700465393	36.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	4.0
13	2.0
14	16.0
15	41.0
16	110.0
17	282.0
18	700.0
19	1467.0
20	2684.0
21	4161.0
22	6640.0
23	10860.0
24	17085.0
25	29856.0
26	42753.0
27	44816.0
28	39218.0
29	34924.0
30	34909.0
31	39200.0
32	47003.0
33	59346.0
34	92943.0
35	137990.0
36	114821.0
37	139898.0
38	200364.0
39	361985.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.043199907126347	22.82609066790498	25.881292446162302	23.24941697880637
2	39.909448187085786	23.126564255252312	25.26723369674703	11.696753860914875
3	25.257536594609913	22.977078645547017	39.258513133768794	12.506871626074279
4	22.114787739311847	25.237459495818975	39.508384237428764	13.139368527440409
5	20.10298049311813	29.134192187003833	38.200982685209524	12.561844634668507
6	18.46915536191702	36.67724015010022	35.188256945890856	9.665347542091911
7	78.40462183008901	2.8888486739895725	16.955792823461525	1.7507366724598885
8	76.49538534030341	5.301310133130286	16.170327550354934	2.0329769762113696
9	73.78347463559042	4.730068869912009	18.518460346022653	2.967996148474926
10	35.53298209792025	26.29000481440634	26.07216146357953	12.104851624093884
11	26.89149830471436	24.23913600185747	33.56003154972667	15.309334143701491
12	23.90308361019015	21.44910216443417	37.5800267011756	17.067787524200075
13	20.478504187850625	25.142878605256243	38.50623653417375	15.872380672719387
14	17.94373632076921	27.835804842405015	38.06645246541993	16.154006371405842
15	17.322302310573598	24.09320144363901	43.12690570251066	15.457590543276734
16	19.503603975811874	22.78723396990484	41.79191521181681	15.91724684246648
17	19.54642146200887	23.173683976904506	36.87992324265632	20.3999713184303
18	20.458358799608018	23.82332153063977	38.336673825677515	17.381645844074693
19	22.23006033373055	26.00448661697471	34.76329168814939	17.00216136114535
20	23.22572053907693	24.895397632404713	34.39534812255225	17.48353370596611
21	21.69494419044562	25.919329670742407	35.78353609609692	16.60219004271505
22	21.07364675915335	23.682850128554893	36.1223883552827	19.121114757009057
23	19.13060699079117	26.347163085454007	35.20888036029515	19.31334956345968
24	19.499984634873375	24.853263040724414	38.51914324053935	17.12760908386286
25	19.50176016061679	25.61387095342318	36.6804497543287	18.203919131631334
26	18.164106381307814	27.82521997739619	35.289666781620575	18.72100685967542
27	18.16875006402136	26.49337080147915	36.314213425023304	19.023665709476187
28	17.88050028852293	26.111018161579675	38.46478483701015	17.543696712887243
29	18.870902206090737	24.40658173735194	37.79165571190046	18.930860344656864
30	19.18708236732213	25.465341396041257	37.740302044244736	17.607274192391873
31	21.28336366523145	25.297690792191784	35.31828006187025	18.100665480706525
32	22.398871858258413	25.713232105602806	34.683734476954015	17.20416155918476
33	21.653833940540373	25.75092788292456	34.16029582990463	18.43494234663043
34	19.463381488778335	27.078543112838076	34.03034100337691	19.427734395006677
35	19.678151814280007	27.846184839058836	33.29978044941288	19.175882897248275
36	23.018530342710612	28.054194508845193	30.265680111721544	18.66159503672265
37	21.845795589184316	29.029436168142286	30.940311604767967	18.184456637905427
38	21.602685141239657	29.509852460639667	29.797897367783083	19.08956503033759
39	21.07569544270344	29.307647394245244	29.478917339033227	20.137739824018084
40	22.142991282851494	27.210819780722574	30.33151114313128	20.31467779329466
41	20.230886636095754	27.143486381376103	30.619351181919686	22.00627580060846
42	20.79796224276217	28.838567150725066	29.24905504471252	21.114415561800246
43	20.691840434867228	27.73965329445387	31.001703821819167	20.566802448859736
44	21.034721771701534	28.625982087676828	30.18236698068433	20.15692915993731
45	19.784205332723282	29.339538568175065	29.57240559836925	21.303850500732405
46	21.226137104732118	29.191009010793145	29.0905552273868	20.492298657087932
47	20.666778206104393	27.917957052763843	30.893874777632472	20.52138996349929
48	21.154979496092135	26.688610343803244	31.560243247026847	20.59616691307777
49	20.91514694182763	26.365669526856532	31.23047348491315	21.48871004640268
50	19.605082100993272	27.822488399329398	31.711367803572223	20.86106169610511
51	19.15983487610586	28.61669472224973	30.593401190285142	21.630069211359267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1287.0
1	1720.0
2	2153.0
3	25057.5
4	47962.0
5	32836.0
6	17710.0
7	18013.5
8	18317.0
9	19693.5
10	21070.0
11	21984.0
12	22898.0
13	23181.0
14	23464.0
15	22547.0
16	21630.0
17	20476.5
18	19323.0
19	18192.0
20	17061.0
21	15947.0
22	14833.0
23	14868.0
24	14903.0
25	14764.0
26	16055.5
27	17486.0
28	18977.5
29	20469.0
30	22761.0
31	25053.0
32	28525.0
33	31997.0
34	35810.5
35	39624.0
36	43566.0
37	47508.0
38	53281.5
39	59055.0
40	65205.0
41	71355.0
42	79137.5
43	86920.0
44	92515.0
45	98110.0
46	105222.5
47	112335.0
48	112798.0
49	113261.0
50	105937.5
51	98614.0
52	90576.5
53	82539.0
54	75240.0
55	67941.0
56	61908.0
57	55875.0
58	51504.5
59	47134.0
60	43577.5
61	40021.0
62	34712.5
63	29404.0
64	25921.5
65	22439.0
66	18292.0
67	14145.0
68	11764.0
69	9383.0
70	8024.5
71	6666.0
72	5404.0
73	4142.0
74	3435.0
75	2118.0
76	1508.0
77	1082.0
78	656.0
79	529.0
80	402.0
81	295.0
82	188.0
83	149.5
84	111.0
85	64.5
86	18.0
87	19.5
88	21.0
89	12.5
90	4.0
91	3.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1464355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.620750314453204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80677808003269	27.76101092212041
2	7.847571183036579	5.747678897377614
3	3.568962834652628	3.92094290548131
4	2.225110271444339	3.2594083069075324
5	1.4893295552031984	2.727018288851599
6	1.158090116316467	2.5446077394756843
7	0.9296230924768587	2.3830446609301417
8	0.7709716546274997	2.2586848370907613
9	0.6497788552811351	2.1415850296975503
>10	5.364480377395668	38.85892918646686
>50	0.16518666719886846	3.813456991091435
>100	0.021629021492907158	1.3816373822135926
>500	0.0011484419266788023	0.28479466484701976
>1k	0.0011484419266788023	0.8042663018593907
>5k	0.0	0.0
>10k+	1.914069877798004E-4	2.1129338855891127
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30144	2.058517231135893	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	3073	0.20985348498144232	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	2505	0.17106507643296878	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG	1811	0.12367219697409439	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	1756	0.11991627713225277	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033871568028244516	0.0
2	0.0	0.0	0.0	0.1702456030129306	0.0
3	0.0	0.0	0.0	0.3884304010980944	0.0
4	0.0	0.0	0.0	0.5734265256717122	0.0
5	0.0	0.0	0.0	1.0156690146856466	0.0
6	0.0	0.0	0.0	1.9215968805378478	0.0
7	0.0	0.0	0.0	2.408432381492193	0.0
8	0.0	0.0	0.0	3.151148457853458	0.0
9	0.0	0.0	0.0	3.631701329254177	0.0
10	0.0	0.0	0.0	4.234560608595594	0.0
11	0.0	0.0	0.0	4.677690860481236	0.0
12	0.0	0.0	0.0	5.047136794015112	0.0
13	0.0	0.0	0.0	5.29967118629021	0.0
14	0.0	0.0	0.0	5.443420482055239	0.0
15	0.0	0.0	0.0	5.5482447903684555	0.0
16	0.0	0.0	0.0	5.70319355620734	0.0
17	0.0	0.0	0.0	5.871185607315166	0.0
18	0.0	0.0	0.0	6.119895790296752	0.0
19	0.0	0.0	0.0	6.2561332463780985	0.0
20	0.0	0.0	0.0	6.398311884754722	0.0
21	0.0	0.0	0.0	6.557562886048807	0.0
22	0.0	0.0	0.0	6.737027565037167	0.0
23	0.0	0.0	0.0	6.938891184173237	0.0
24	0.0	0.0	0.0	7.107361261442751	0.0
25	0.0	0.0	0.0	7.249676478722714	0.0
26	0.0	0.0	0.0	7.395611036941179	0.0
27	0.0	0.0	0.0	7.5606666416272015	0.0
28	0.0	0.0	0.0	7.707693831072383	0.0
29	0.0	0.0	0.0	7.879168644215372	0.0
30	0.0	0.0	0.0	8.118318303963179	0.0
31	0.0	0.0	0.0	8.29293443188298	0.0
32	0.0	0.0	0.0	8.474584373324774	0.0
33	0.0	0.0	0.0	8.659307340091713	0.0
34	0.0	0.0	0.0	8.85338596173742	0.0
35	0.0	0.0	0.0	9.051971687193337	0.0
36	0.0	0.0	0.0	9.250352544294246	0.0
37	0.0	0.0	0.0	9.452352742333655	0.0
38	0.0	0.0	0.0	9.674156881357321	0.0
39	0.0	0.0	0.0	9.923276801048926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCG	30	2.1659125E-6	44.999996	1
CGTTTTT	20325	0.0	44.02583	1
GCGTAAG	90	0.0	42.5	1
ACGGGTA	160	0.0	40.78125	5
TATACGG	110	0.0	38.863632	2
GTTTTTT	22775	0.0	38.400658	2
GCGAGAC	165	0.0	38.181816	21
GCGATAC	95	0.0	37.894737	9
AGGGTAC	685	0.0	37.445255	6
AGGGCGA	1595	0.0	37.382446	6
GGGCGAT	2895	0.0	37.07254	7
GGCGATT	820	0.0	36.76829	8
CCTCGTC	215	0.0	36.627907	36
TAGGGAC	1070	0.0	36.588783	5
TGGGCGA	745	0.0	36.543625	6
GCGATTG	420	0.0	36.42857	9
TTAGGGA	1375	0.0	36.32727	4
GGCACCG	685	0.0	36.131386	8
TAATACG	25	0.0021072968	36.0	1
CGTAAGA	25	0.0021072968	36.0	27
>>END_MODULE