FastQCFastQC Report
Thu 26 May 2016
SRR1547112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547112_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1233244
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59850.4853054221224672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA600.045.00000414
TATGCGT253.890972E-545.011
ACGATAC207.033487E-445.045
TAACACG253.890972E-545.021
CTGTACG900.040.01
AATACGG451.9284926E-840.02
GCGTAAG1750.039.8571431
ACGGGAT2550.039.7058835
CGTTTTT39400.039.6890871
CGGTCTA1200.039.37500431
GGCGATT7450.038.657728
ACGTAGG1350.038.3333322
TGTAGCG601.5643309E-1037.5000041
TAAGGGC5650.037.4336284
AGGGATC6750.037.3333326
TTACACG9150.037.1311534
TAAGGGA12250.037.1020434
CGACGGG1700.037.0588233
ACACGCG9100.036.8406636
ATCACGG1100.036.818182
Produced by FastQC (version 0.11.5)