##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547112_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1233244
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45619115114284	31.0	31.0	33.0	30.0	34.0
2	31.823889676333312	31.0	31.0	34.0	30.0	34.0
3	31.955192159864552	33.0	31.0	34.0	30.0	34.0
4	35.70496187291404	37.0	35.0	37.0	33.0	37.0
5	35.64186000499495	37.0	35.0	37.0	33.0	37.0
6	35.68233536915647	37.0	35.0	37.0	33.0	37.0
7	36.00811031717973	37.0	35.0	37.0	35.0	37.0
8	34.69813597309211	37.0	35.0	37.0	33.0	37.0
9	37.0084557476055	39.0	37.0	39.0	34.0	39.0
10	36.913128302266216	39.0	37.0	39.0	32.0	39.0
11	36.84527473881892	39.0	37.0	39.0	32.0	39.0
12	36.48740881771977	39.0	35.0	39.0	32.0	39.0
13	36.38680180077908	39.0	35.0	39.0	32.0	39.0
14	37.412458524022824	40.0	35.0	41.0	32.0	41.0
15	37.60211199081447	40.0	35.0	41.0	33.0	41.0
16	37.66250068923911	40.0	35.0	41.0	33.0	41.0
17	37.5700453438249	39.0	35.0	41.0	33.0	41.0
18	37.52979702313573	39.0	36.0	41.0	33.0	41.0
19	37.47182714856103	39.0	36.0	41.0	32.0	41.0
20	37.36050124711736	39.0	35.0	41.0	32.0	41.0
21	37.2624249540237	39.0	35.0	41.0	32.0	41.0
22	37.18277080610163	39.0	35.0	41.0	32.0	41.0
23	37.11646032739669	39.0	35.0	41.0	32.0	41.0
24	36.98457482866326	39.0	35.0	41.0	32.0	41.0
25	36.8875234746733	39.0	35.0	41.0	31.0	41.0
26	36.768912721245755	38.0	35.0	41.0	31.0	41.0
27	36.65755600675941	38.0	35.0	41.0	31.0	41.0
28	36.68828796247944	38.0	35.0	41.0	31.0	41.0
29	36.649631378705266	38.0	35.0	41.0	31.0	41.0
30	36.59281456062223	38.0	35.0	41.0	31.0	41.0
31	36.49598376314825	38.0	35.0	41.0	31.0	41.0
32	36.44562471011414	38.0	35.0	41.0	30.0	41.0
33	36.35863219281829	38.0	35.0	41.0	30.0	41.0
34	36.206459548961924	38.0	35.0	41.0	30.0	41.0
35	36.103862658160104	38.0	35.0	41.0	30.0	41.0
36	35.984278861279684	38.0	35.0	41.0	30.0	41.0
37	35.961926431428004	38.0	35.0	41.0	30.0	41.0
38	35.840168693299944	38.0	35.0	41.0	29.0	41.0
39	35.757301069374755	38.0	35.0	40.0	29.0	41.0
40	35.6015127582214	38.0	35.0	40.0	28.0	41.0
41	35.51651660174304	38.0	35.0	40.0	28.0	41.0
42	35.45062453172284	38.0	34.0	40.0	28.0	41.0
43	35.355602784201665	37.0	34.0	40.0	28.0	41.0
44	35.232692800451495	37.0	34.0	40.0	27.0	41.0
45	35.10163925387028	37.0	34.0	40.0	27.0	41.0
46	35.02078258641437	37.0	34.0	40.0	27.0	41.0
47	34.944757079701986	37.0	34.0	40.0	27.0	41.0
48	34.876113729318774	36.0	34.0	40.0	27.0	41.0
49	34.79497406839198	36.0	34.0	40.0	27.0	41.0
50	34.70204598603358	36.0	34.0	40.0	26.0	41.0
51	34.339068343328655	35.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	8.0
14	16.0
15	31.0
16	94.0
17	222.0
18	468.0
19	881.0
20	1571.0
21	2502.0
22	3858.0
23	5632.0
24	8056.0
25	11367.0
26	15026.0
27	18330.0
28	20589.0
29	23831.0
30	28376.0
31	34828.0
32	42653.0
33	56317.0
34	93213.0
35	135486.0
36	101089.0
37	133149.0
38	187768.0
39	307623.0
40	255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.717181676943085	23.411587650132496	27.50850602151723	13.362724651407182
2	31.763057432268067	25.47598042236573	28.6776177301491	14.083344415217102
3	30.106532040699165	25.605719549416012	30.322466600283484	13.965281809601343
4	26.062644537496226	28.490387952424662	29.64725553094116	15.799711979137948
5	23.46826743126259	32.069971554696394	29.021102069014727	15.44065894502629
6	21.990376600251047	40.79995524000117	26.63098300093088	10.578685158816908
7	88.28666508817395	3.685077729954494	6.256912662863148	1.771344519008404
8	85.88576145515405	5.903049193833499	5.8202594133845365	2.3909299376279147
9	83.1297780487884	5.167428343458392	8.372390216372429	3.330403391380781
10	43.36189756447224	29.156841630691087	14.697740268754602	12.783520536082074
11	36.401636659087735	24.30549023550895	22.460599848853917	16.832273256549392
12	31.78584286645627	22.195526594899306	26.71085365102121	19.307776887623213
13	24.913885654420373	28.336484912961264	28.56239316793757	18.18723626468079
14	20.873484890256915	29.920924002062854	30.205214864211786	19.000376243468445
15	19.758863614986165	23.305201565951265	37.25945554975333	19.676479269309237
16	23.364151781804736	21.287839227273757	34.85644365591886	20.491565335002644
17	23.108646788470082	21.87774682058052	28.239829263308803	26.773777127640596
18	25.526740855824155	21.9292370366286	30.49664137834849	22.047380729198764
19	27.438771240727704	24.675327834556665	26.255955836801153	21.629945087914475
20	29.379992929217575	24.51064023015721	25.33634868687786	20.77301815374735
21	26.66974256513715	25.74283758931728	27.423364719390484	20.164055126155084
22	26.489810613309288	22.584743976050156	27.06852820690796	23.856917203732593
23	23.83469937822523	26.700798868674813	26.185085838649936	23.27941591445002
24	22.953770705553808	24.65205587864202	31.394760485354073	20.9994129304501
25	24.304355018147263	24.915588480462908	28.819276639497133	21.9607798618927
26	22.1487394222068	27.182374290894586	26.852999082095675	23.815887204802944
27	22.62666593147828	26.010019104086457	27.929104054023373	23.434210910411892
28	20.945490105769824	26.88129842918352	30.505479856378788	21.66773160866787
29	22.04867812046927	24.994972608826803	29.80813204848351	23.14821722222042
30	23.681526121351492	25.796030631407895	29.376587277132504	21.145855970108105
31	27.500072978258967	25.095033910564336	25.418895206463603	21.9859979047131
32	27.4853962395114	25.61982867948273	25.818086283006448	21.076688797999424
33	26.836457343396763	25.037137825118144	25.34867390394764	22.777730927537455
34	22.977204835377265	26.79250821410848	27.795391666207177	22.43489528430708
35	23.01491026917625	26.141785404996902	28.527120342770772	22.31618398305607
36	27.624460366318427	26.18330192565299	25.132577170454507	21.059660537574075
37	23.334392869537577	28.39713795485727	26.156218882881248	22.112250292723907
38	24.250918715193425	28.203907742506757	25.29507542708499	22.25009811521483
39	23.469564822533094	26.61671169695535	25.66929172167065	24.24443175884091
40	25.63482976604792	24.616620879566412	26.46791713561955	23.280632218766115
41	21.671299434661755	24.081771328301617	27.683897103898335	26.563032133138293
42	23.394964824479178	25.713646285730967	25.936067801667797	24.95532108812206
43	23.319472869926795	24.797201527029525	27.39117319849114	24.492152404552545
44	23.12332352721765	25.608071071093796	27.866423838267206	23.40218156342135
45	22.253747028163122	26.59960234957559	26.210952577105584	24.935698045155704
46	23.670984817278658	26.22635909844281	26.96019603582097	23.142460048457565
47	22.86862940342706	25.989179756804003	28.1388760050728	23.003314834696134
48	23.609764166701805	25.070221302515964	28.404516867708256	22.915497663073975
49	23.87151285552575	24.048201329177356	29.04040076416346	23.03988505113343
50	22.344483330143913	26.982494948282742	27.870397099033116	22.802624622540225
51	22.224393550667994	27.20491646421957	26.602278219071003	23.96841176604143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	969.0
1	1582.5
2	2196.0
3	6394.0
4	10592.0
5	7366.5
6	4141.0
7	4240.5
8	4340.0
9	4536.5
10	4733.0
11	4840.0
12	4947.0
13	4937.5
14	4928.0
15	4769.0
16	4610.0
17	4694.5
18	4779.0
19	4527.0
20	4275.0
21	4567.5
22	4860.0
23	5537.5
24	6215.0
25	6126.5
26	7456.5
27	8875.0
28	10574.5
29	12274.0
30	14158.5
31	16043.0
32	18448.0
33	20853.0
34	23505.0
35	26157.0
36	30788.5
37	35420.0
38	38548.0
39	41676.0
40	49977.0
41	58278.0
42	66418.0
43	74558.0
44	77329.0
45	80100.0
46	83973.5
47	87847.0
48	91277.5
49	94708.0
50	93570.0
51	92432.0
52	88781.5
53	85131.0
54	80721.5
55	76312.0
56	72973.5
57	69635.0
58	65020.5
59	60406.0
60	57895.0
61	55384.0
62	50111.5
63	44839.0
64	41866.0
65	38893.0
66	33553.0
67	28213.0
68	23881.5
69	19550.0
70	16666.0
71	13782.0
72	11948.5
73	10115.0
74	8475.5
75	5270.0
76	3704.0
77	2876.5
78	2049.0
79	1453.5
80	858.0
81	625.0
82	392.0
83	278.5
84	165.0
85	128.0
86	91.0
87	61.5
88	32.0
89	18.0
90	4.0
91	4.0
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1233244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.556853403285224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83902384205024	19.38206814579531
2	6.4441399562532435	3.29383880344434
3	2.658389945351234	2.0382024636632665
4	1.6093407414837966	1.6451874162414293
5	1.1617659959380977	1.484554162355581
6	0.9458969745103067	1.4504490187302557
7	0.7747363646238956	1.3859876587820918
8	0.6658462677797036	1.3613548363816386
9	0.5616605212010419	1.291884804247302
>10	8.52328372569524	51.601554009662735
>50	0.7704599335694587	12.219408893035396
>100	0.0425125546811736	1.9164797174766373
>500	0.002289137559741495	0.32761765699189777
>1k	3.2701965139164213E-4	0.10121045474928271
>5k	3.2701965139164213E-4	0.5002019584429868
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5985	0.4853054221224672	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.108695440642728E-5	0.0	0.0	0.0454897814220057	0.0
2	8.108695440642728E-5	0.0	0.0	0.13209064872807003	0.0
3	8.108695440642728E-5	0.0	0.0	0.21033955973027235	0.0
4	8.108695440642728E-5	0.0	0.0	0.34883607785645016	0.0
5	8.108695440642728E-5	0.0	0.0	0.573122593744628	0.0
6	8.108695440642728E-5	0.0	0.0	1.0260743210589307	0.0
7	8.108695440642728E-5	0.0	0.0	1.2361706199259839	0.0
8	8.108695440642728E-5	0.0	0.0	1.7606410410267554	0.0
9	8.108695440642728E-5	0.0	0.0	2.0242547298020503	0.0
10	8.108695440642728E-5	0.0	0.0	2.415256023949843	0.0
11	8.108695440642728E-5	0.0	0.0	2.783634057818242	0.0
12	8.108695440642728E-5	0.0	0.0	3.105873614629384	0.0
13	8.108695440642728E-5	0.0	0.0	3.2762373058372876	0.0
14	8.108695440642728E-5	0.0	0.0	3.3603244775567527	0.0
15	8.108695440642728E-5	0.0	0.0	3.443519692777747	0.0
16	8.108695440642728E-5	0.0	0.0	3.586394906441872	0.0
17	8.108695440642728E-5	0.0	0.0	3.7492985978443842	0.0
18	8.108695440642728E-5	0.0	0.0	3.9482048970033503	0.0
19	8.108695440642728E-5	0.0	0.0	4.056861415907963	0.0
20	8.108695440642728E-5	0.0	0.0	4.171923804210683	0.0
21	8.108695440642728E-5	0.0	0.0	4.327367495807804	0.0
22	8.108695440642728E-5	0.0	0.0	4.485000535173899	0.0
23	8.108695440642728E-5	0.0	0.0	4.660959226235846	0.0
24	8.108695440642728E-5	0.0	0.0	4.805131831170474	0.0
25	8.108695440642728E-5	0.0	0.0	4.927735306232992	0.0
26	8.108695440642728E-5	0.0	0.0	5.043203129307744	0.0
27	8.108695440642728E-5	0.0	0.0	5.187456821196778	0.0
28	8.108695440642728E-5	0.0	0.0	5.31792573083672	0.0
29	8.108695440642728E-5	0.0	0.0	5.464612031357947	0.0
30	8.108695440642728E-5	0.0	0.0	5.662139852292004	0.0
31	8.108695440642728E-5	0.0	0.0	5.810934413627798	0.0
32	8.108695440642728E-5	0.0	0.0	5.988028322051435	0.0
33	8.108695440642728E-5	0.0	0.0	6.146066796189562	0.0
34	8.108695440642728E-5	0.0	0.0	6.31270048749477	0.0
35	8.108695440642728E-5	0.0	0.0	6.505930699845286	0.0
36	1.6217390881285456E-4	0.0	0.0	6.668591130384579	0.0
37	1.6217390881285456E-4	0.0	0.0	6.840414386771799	0.0
38	1.6217390881285456E-4	0.0	0.0	7.048807859596317	0.0
39	1.6217390881285456E-4	0.0	0.0	7.404130893805281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	60	0.0	45.000004	14
TATGCGT	25	3.890972E-5	45.0	11
ACGATAC	20	7.033487E-4	45.0	45
TAACACG	25	3.890972E-5	45.0	21
CTGTACG	90	0.0	40.0	1
AATACGG	45	1.9284926E-8	40.0	2
GCGTAAG	175	0.0	39.857143	1
ACGGGAT	255	0.0	39.705883	5
CGTTTTT	3940	0.0	39.689087	1
CGGTCTA	120	0.0	39.375004	31
GGCGATT	745	0.0	38.65772	8
ACGTAGG	135	0.0	38.333332	2
TGTAGCG	60	1.5643309E-10	37.500004	1
TAAGGGC	565	0.0	37.433628	4
AGGGATC	675	0.0	37.333332	6
TTACACG	915	0.0	37.13115	34
TAAGGGA	1225	0.0	37.102043	4
CGACGGG	170	0.0	37.058823	3
ACACGCG	910	0.0	36.84066	36
ATCACGG	110	0.0	36.81818	2
>>END_MODULE