FastQCFastQC Report
Thu 26 May 2016
SRR1547111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547111_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences710237
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.41662149395201886No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8360.11770718788235476No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT207.0314633E-445.00000441
TATCACG207.0314633E-445.0000041
ATTAGCG302.1643245E-645.0000041
TAGACCG351.2110468E-745.00000441
CGTCTTA207.0314633E-445.00000445
TAACACG406.8084773E-945.0000041
GCGATCG207.0314633E-445.0000049
TATAGCG302.1643245E-645.0000041
AATACGG453.8380676E-1045.02
CGGTCTA253.8892962E-544.99999631
CAGTACG253.8892962E-544.9999961
GCGTAAC253.8892962E-544.99999632
TCGTAAG850.042.352941
GCGATAT700.041.7857179
GCGATAA700.041.7857179
TACGGGA1350.041.6666644
TTAGGCG501.0804797E-940.4999961
CACGGGT501.0804797E-940.4999964
TATGGGT1450.040.3448264
TAAGACG451.9264917E-840.01
Produced by FastQC (version 0.11.5)