FastQCFastQC Report
Thu 26 May 2016
SRR1547110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547110_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1154651
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87310.7561592204051268No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA207.0333004E-445.031
TAATGCG406.8157533E-945.01
CGTTTTT68850.043.1045761
GTCAACG451.9283107E-840.01
CGGGTAT1250.039.6000026
CTAAGCG800.039.3751
GCCGATA750.039.09
CTAACGG700.038.5714262
AGGGCGA14550.038.5051546
TAGGGTC9000.038.2500045
GGGCGAT28850.037.9029467
TTAACGG850.037.0588232
TAGTGCG850.037.0588231
GCGATGT3900.036.9230779
CGAATAT800.036.562514
TAGGGCG6100.036.5163965
GGCGATT7900.036.4556968
ATAGGGT10100.036.3118824
TGGGCGA8800.036.0511366
TGTAGCG1250.036.0000041
Produced by FastQC (version 0.11.5)