##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547110_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1154651
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47091805229459	31.0	31.0	33.0	30.0	34.0
2	31.84456169006912	31.0	31.0	34.0	30.0	34.0
3	31.91950727968884	33.0	31.0	34.0	30.0	34.0
4	35.696193914871245	37.0	35.0	37.0	33.0	37.0
5	35.65037920549153	37.0	35.0	37.0	33.0	37.0
6	35.70589381553387	37.0	35.0	37.0	33.0	37.0
7	36.0750434546889	37.0	35.0	37.0	35.0	37.0
8	34.76830228354715	37.0	35.0	37.0	35.0	37.0
9	37.08052303250073	39.0	37.0	39.0	34.0	39.0
10	36.7915300813839	39.0	37.0	39.0	32.0	39.0
11	36.868664211090625	39.0	37.0	39.0	33.0	39.0
12	36.79873139156334	39.0	35.0	39.0	33.0	39.0
13	36.80413648799507	39.0	35.0	39.0	33.0	39.0
14	37.94750881435169	40.0	37.0	41.0	33.0	41.0
15	38.04940107443721	40.0	37.0	41.0	33.0	41.0
16	38.0424812345895	40.0	37.0	41.0	33.0	41.0
17	37.960891213015884	40.0	36.0	41.0	33.0	41.0
18	37.86494966877437	39.0	36.0	41.0	33.0	41.0
19	37.777100613085686	39.0	36.0	41.0	33.0	41.0
20	37.6363870987857	39.0	35.0	41.0	33.0	41.0
21	37.565628055576965	39.0	35.0	41.0	33.0	41.0
22	37.474682826239274	39.0	35.0	41.0	32.0	41.0
23	37.38173439420223	39.0	35.0	41.0	32.0	41.0
24	37.26282660301684	39.0	35.0	41.0	32.0	41.0
25	37.190268747872736	39.0	35.0	41.0	32.0	41.0
26	37.10164976256895	39.0	35.0	41.0	32.0	41.0
27	37.01180616480651	39.0	35.0	41.0	32.0	41.0
28	37.02239204746716	39.0	35.0	41.0	32.0	41.0
29	36.988301226950824	39.0	35.0	41.0	31.0	41.0
30	36.927593705803744	39.0	35.0	41.0	31.0	41.0
31	36.81661471734749	39.0	35.0	41.0	31.0	41.0
32	36.68996952325854	39.0	35.0	41.0	31.0	41.0
33	36.47950592863125	39.0	35.0	41.0	30.0	41.0
34	36.26420970492383	39.0	35.0	41.0	30.0	41.0
35	36.10714839375707	39.0	35.0	41.0	29.0	41.0
36	35.98600702723161	39.0	35.0	41.0	28.0	41.0
37	35.95114541103762	39.0	35.0	41.0	28.0	41.0
38	35.86553512706437	39.0	35.0	41.0	27.0	41.0
39	35.79624319383086	39.0	35.0	41.0	27.0	41.0
40	35.69462893982684	39.0	35.0	41.0	27.0	41.0
41	35.6181928565428	39.0	35.0	41.0	26.0	41.0
42	35.56852243664969	39.0	35.0	41.0	26.0	41.0
43	35.49727406809504	39.0	35.0	41.0	26.0	41.0
44	35.41030493196646	38.0	35.0	41.0	26.0	41.0
45	35.28972564004188	38.0	35.0	40.0	25.0	41.0
46	35.22139676837417	38.0	34.0	40.0	24.0	41.0
47	35.159805863416736	38.0	34.0	40.0	24.0	41.0
48	35.078594311181476	38.0	34.0	40.0	24.0	41.0
49	35.01737321493681	38.0	34.0	40.0	24.0	41.0
50	34.929976243904	38.0	34.0	40.0	24.0	41.0
51	34.582455651101505	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	2.0
14	2.0
15	18.0
16	73.0
17	149.0
18	380.0
19	794.0
20	1417.0
21	2235.0
22	3599.0
23	5373.0
24	8279.0
25	12957.0
26	18191.0
27	20462.0
28	20731.0
29	21701.0
30	23957.0
31	29074.0
32	35616.0
33	45857.0
34	69385.0
35	100043.0
36	87774.0
37	116460.0
38	181438.0
39	348469.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.82342456725019	23.562444409609483	27.459985744610275	16.15414527853005
2	34.04838345093019	24.879898774608087	27.983174136600585	13.08854363786114
3	28.817192381074452	25.266335888506568	32.939823375201684	12.976648355217291
4	24.347010481955152	28.34908556784691	32.78843563986001	14.515468310337928
5	21.985431095629764	32.309762863410675	31.889202884681172	13.815603156278392
6	20.681920337833684	40.98164726830878	28.33167771040773	10.004754683449804
7	87.30542822030206	2.9606348584983686	8.525260013631824	1.2086769075677413
8	85.07471088666618	5.218719769003794	7.9617131063845274	1.7448562379454917
9	82.06592294987836	4.1973721929829875	10.877312711806425	2.859392145332226
10	31.64843749323389	26.864654341441703	26.989540562472992	14.497367602851424
11	29.048084659347285	25.641600795391856	27.300716839980222	18.009597705280644
12	26.743578795670725	24.639999445719962	29.66143016374645	18.954991594862864
13	23.690448455853762	27.181286813071655	31.14863279034098	17.979631940733608
14	21.49575932467906	28.997679818404006	31.237923840190675	18.268637016726267
15	21.13625675637054	25.41685756128908	35.96688523198785	17.48000045035253
16	22.527846076433487	24.125038648041702	35.26632722788098	18.080788047643832
17	22.291151179014264	24.163751644436285	31.725776879767132	21.81932029678232
18	23.292146284894745	24.612631868850414	32.69732585863607	19.397895987618767
19	24.90449495128831	26.917137732526967	29.482068607743813	18.696298708440906
20	26.264126562918143	26.1676471938274	28.962084647222408	18.606141596032046
21	24.649006496335257	26.627786231510647	30.706074822608738	18.01713244954536
22	23.82910507157574	24.568462678333105	30.38363973183239	21.218792518258763
23	21.81247840256493	27.656235520516585	30.15066890341757	20.380617173500912
24	20.971618263873673	26.620424699757763	33.67987383200638	18.728083204362182
25	21.50545922534168	27.18137341932757	31.4991283080342	19.814039047296543
26	20.482812555482134	29.11511790142649	30.194578275167128	20.207491267924247
27	20.685384588070335	27.754100589701995	31.12611516380274	20.434399658424926
28	19.73219613545565	28.32847327893883	32.760721637966796	19.178608947638722
29	20.407205294067214	25.979538405977216	32.81277199777249	20.800484302183083
30	21.374856991419918	26.818926238317896	32.22211733242339	19.58409943783879
31	22.903197589574688	27.218267684347914	29.849192526572963	20.029342199504438
32	23.344196644700432	27.23810051695274	29.875087797091936	19.54261504125489
33	22.461332471889776	27.403691678264686	29.178860105780878	20.956115744064657
34	20.34346308971282	28.620163148864897	30.072376848069243	20.96399691335304
35	20.92450446065521	28.484018114564485	29.671303276920906	20.920174147859395
36	22.836164347495476	29.14179262824871	27.75210864581592	20.269934378439892
37	21.60167877566468	29.581405983279797	28.551744206691026	20.265171034364496
38	21.41582175046832	29.840531900981336	28.272092606337324	20.471553742213015
39	21.38013997303081	28.916269937842692	28.09403014417343	21.609559944953062
40	22.424871238149017	27.60184679180116	28.759685827146036	21.213596142903786
41	20.147126707550594	27.575691702514437	29.192024256680156	23.08515733325481
42	21.608174244858404	28.254598142642234	27.938225489780027	22.19900212271933
43	21.34558407692021	27.79489213623857	28.65489225748733	22.20463152935389
44	21.419545819472724	29.14577651602086	28.654719044975497	20.779958619530923
45	20.752850861429124	29.052934609678594	28.29331113903682	21.900903389855465
46	22.02466372955984	28.950219590161876	28.19198181961476	20.833134860663524
47	21.699370632338255	28.037995896595596	28.988759374044626	21.273874097021526
48	21.666027223810485	27.55975615142584	29.395375745571606	21.37884087919207
49	21.568508579648743	27.19704915164842	29.289196475818237	21.945245792884606
50	21.011976779130663	28.593055390763094	29.04886411565053	21.34610371445571
51	20.579638349596543	28.636964762512658	28.67238672118242	22.111010166708382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	678.0
1	892.0
2	1106.0
3	8466.0
4	15826.0
5	11353.0
6	6880.0
7	6919.5
8	6959.0
9	7156.5
10	7354.0
11	7713.5
12	8073.0
13	7879.0
14	7685.0
15	7552.0
16	7419.0
17	7153.5
18	6888.0
19	6648.0
20	6408.0
21	6311.0
22	6214.0
23	6464.0
24	6714.0
25	7581.0
26	10100.0
27	11752.0
28	14250.0
29	16748.0
30	18869.0
31	20990.0
32	24121.5
33	27253.0
34	31651.0
35	36049.0
36	40086.0
37	44123.0
38	48742.5
39	53362.0
40	60620.0
41	67878.0
42	73351.0
43	78824.0
44	82646.5
45	86469.0
46	90542.0
47	94615.0
48	96347.5
49	98080.0
50	94022.0
51	89964.0
52	84557.0
53	79150.0
54	71716.5
55	64283.0
56	57722.5
57	51162.0
58	46113.0
59	41064.0
60	36417.0
61	31770.0
62	27488.0
63	23206.0
64	19273.0
65	15340.0
66	12359.5
67	9379.0
68	7816.0
69	6253.0
70	5056.0
71	3859.0
72	3162.5
73	2466.0
74	2177.0
75	1568.5
76	1249.0
77	854.5
78	460.0
79	320.0
80	180.0
81	146.0
82	112.0
83	73.5
84	35.0
85	28.0
86	21.0
87	16.5
88	12.0
89	6.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1154651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.598674540319443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.47308031544115	22.900532778220565
2	6.860043106421751	4.3353653890476584
3	3.549009136136333	3.3643195390017673
4	2.375854023886955	3.002953522244489
5	1.7805472862580412	2.8131467101058427
6	1.4855396358167154	2.8164650081350238
7	1.2316263125584235	2.724243130407916
8	1.0408460642384265	2.6311484824353975
9	0.9154433758040851	2.6034117562912162
>10	8.173905319279157	49.19885010407898
>50	0.0984062833569267	1.9019241680465622
>100	0.0151384572038714	0.8694548345479237
>500	2.803417990757419E-4	0.06475511795669699
>1k	0.0	0.0
>5k	2.803417990757419E-4	0.7734294594800566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8731	0.7561592204051268	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03204431468902725	0.0
2	0.0	0.0	0.0	0.09890434425640302	0.0
3	0.0	0.0	0.0	0.17615712453373358	0.0
4	0.0	0.0	0.0	0.2953273326745484	0.0
5	0.0	0.0	0.0	0.4943485087701825	0.0
6	0.0	0.0	0.0	0.9862720423747089	0.0
7	0.0	0.0	0.0	1.2369105469964516	0.0
8	0.0	0.0	0.0	1.8290375186961254	0.0
9	0.0	0.0	0.0	2.155543103500538	0.0
10	0.0	0.0	0.0	2.608407215686818	0.0
11	0.0	0.0	0.0	2.921575437080122	0.0
12	0.0	0.0	0.0	3.199841337339161	0.0
13	0.0	0.0	0.0	3.3654324986511077	0.0
14	0.0	0.0	0.0	3.463557386604264	0.0
15	0.0	0.0	0.0	3.546093148492488	0.0
16	0.0	0.0	0.0	3.6788605388121605	0.0
17	0.0	0.0	0.0	3.825744748846188	0.0
18	0.0	0.0	0.0	4.022167737264334	0.0
19	0.0	0.0	0.0	4.12964610085645	0.0
20	0.0	0.0	0.0	4.250375221603758	0.0
21	0.0	0.0	0.0	4.397346037893701	0.0
22	0.0	0.0	0.0	4.557221186315172	0.0
23	0.0	0.0	0.0	4.736582742317808	0.0
24	0.0	0.0	0.0	4.88502586495833	0.0
25	0.0	0.0	0.0	5.014069186273601	0.0
26	0.0	0.0	0.0	5.130294781713262	0.0
27	0.0	0.0	0.0	5.266006784734088	0.0
28	0.0	0.0	0.0	5.412544569744451	0.0
29	0.0	0.0	0.0	5.562113573711883	0.0
30	0.0	0.0	0.0	5.750222361562065	0.0
31	0.0	0.0	0.0	5.915640310362178	0.0
32	0.0	0.0	0.0	6.077940433949306	0.0
33	0.0	0.0	0.0	6.239894132512768	0.0
34	0.0	0.0	0.0	6.40955578785278	0.0
35	0.0	0.0	0.0	6.5929012316275655	0.0
36	0.0	0.0	0.0	6.77113690630329	0.0
37	0.0	0.0	0.0	6.943483355576706	0.0
38	0.0	0.0	0.0	7.138520643900192	0.0
39	0.0	0.0	0.0	7.377467303973235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	20	7.0333004E-4	45.0	31
TAATGCG	40	6.8157533E-9	45.0	1
CGTTTTT	6885	0.0	43.104576	1
GTCAACG	45	1.9283107E-8	40.0	1
CGGGTAT	125	0.0	39.600002	6
CTAAGCG	80	0.0	39.375	1
GCCGATA	75	0.0	39.0	9
CTAACGG	70	0.0	38.571426	2
AGGGCGA	1455	0.0	38.505154	6
TAGGGTC	900	0.0	38.250004	5
GGGCGAT	2885	0.0	37.902946	7
TTAACGG	85	0.0	37.058823	2
TAGTGCG	85	0.0	37.058823	1
GCGATGT	390	0.0	36.923077	9
CGAATAT	80	0.0	36.5625	14
TAGGGCG	610	0.0	36.516396	5
GGCGATT	790	0.0	36.455696	8
ATAGGGT	1010	0.0	36.311882	4
TGGGCGA	880	0.0	36.051136	6
TGTAGCG	125	0.0	36.000004	1
>>END_MODULE