FastQCFastQC Report
Thu 26 May 2016
SRR1547109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547109_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1218741
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73200.6006198199617474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG351.2120108E-745.0000041
CTATACG207.033454E-445.01
CGGTCTA950.042.6315831
AGGGCGA15250.039.0983626
CGTTTTT50200.038.904381
GGGCGAT30500.037.696727
TAGTACG301.1399625E-437.4999961
TTACACG7200.036.87534
TCACGAC1100.036.8181825
CTCACGA1100.036.8181824
CACGACG1100.036.8181826
TCAAGCG8500.036.79411717
GGCGATT7100.036.4436658
ATACTAT5550.036.0810845
CGTATAA250.002107158536.026
GCGAATA250.002107158536.025
TTATGCG504.879439E-836.01
CATAGCG504.879439E-836.01
ACTAACG250.002107158536.01
TATAGCG504.879439E-836.01
Produced by FastQC (version 0.11.5)