##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547109_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1218741
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506250302566336	31.0	31.0	33.0	30.0	34.0
2	31.87666698666903	31.0	31.0	34.0	30.0	34.0
3	31.97042521749904	33.0	31.0	34.0	30.0	34.0
4	35.725966386623575	37.0	35.0	37.0	33.0	37.0
5	35.676553098648526	37.0	35.0	37.0	33.0	37.0
6	35.72549458826773	37.0	35.0	37.0	33.0	37.0
7	36.05356347246872	37.0	35.0	37.0	35.0	37.0
8	34.73446942377421	37.0	35.0	37.0	33.0	37.0
9	37.046800755861995	39.0	37.0	39.0	34.0	39.0
10	36.97338729065486	39.0	37.0	39.0	32.0	39.0
11	36.914821114576434	39.0	37.0	39.0	33.0	39.0
12	36.67925670835723	39.0	35.0	39.0	32.0	39.0
13	36.643037363968226	39.0	35.0	39.0	33.0	39.0
14	37.76324830296183	40.0	36.0	41.0	33.0	41.0
15	37.89623554143169	40.0	36.0	41.0	33.0	41.0
16	37.90733470031779	40.0	36.0	41.0	33.0	41.0
17	37.792421851730595	40.0	36.0	41.0	33.0	41.0
18	37.71590682515809	39.0	36.0	41.0	33.0	41.0
19	37.65958476821572	39.0	36.0	41.0	33.0	41.0
20	37.54870230836576	39.0	35.0	41.0	33.0	41.0
21	37.454784896873086	39.0	35.0	41.0	32.0	41.0
22	37.35276486144308	39.0	35.0	41.0	32.0	41.0
23	37.251692525319164	39.0	35.0	41.0	32.0	41.0
24	37.1146494620268	39.0	35.0	41.0	32.0	41.0
25	36.99626335702171	39.0	35.0	41.0	31.0	41.0
26	36.90665366964761	39.0	35.0	41.0	31.0	41.0
27	36.81538325206094	39.0	35.0	41.0	31.0	41.0
28	36.83675284576461	39.0	35.0	41.0	31.0	41.0
29	36.77080938443853	39.0	35.0	41.0	31.0	41.0
30	36.704297303528804	39.0	35.0	41.0	31.0	41.0
31	36.62005873274141	39.0	35.0	41.0	31.0	41.0
32	36.52275586035097	39.0	35.0	41.0	30.0	41.0
33	36.397523345813426	39.0	35.0	41.0	30.0	41.0
34	36.22687593180175	39.0	35.0	41.0	30.0	41.0
35	36.09478552046743	39.0	35.0	41.0	30.0	41.0
36	35.96146268977576	38.0	35.0	41.0	29.0	41.0
37	35.92886101312748	38.0	35.0	41.0	29.0	41.0
38	35.80189884479147	38.0	35.0	41.0	29.0	41.0
39	35.71264854468669	38.0	35.0	41.0	28.0	41.0
40	35.566906340231434	38.0	35.0	40.0	28.0	41.0
41	35.45900482547153	38.0	35.0	40.0	27.0	41.0
42	35.380892248640194	38.0	35.0	40.0	27.0	41.0
43	35.27829784999438	38.0	34.0	40.0	27.0	41.0
44	35.14435306599187	38.0	34.0	40.0	26.0	41.0
45	35.02232713923631	37.0	34.0	40.0	26.0	41.0
46	34.947362893346494	37.0	34.0	40.0	26.0	41.0
47	34.86740825163017	37.0	34.0	40.0	26.0	41.0
48	34.782978499943795	37.0	34.0	40.0	26.0	41.0
49	34.69530031401258	36.0	34.0	40.0	26.0	41.0
50	34.5957442967784	36.0	34.0	40.0	26.0	41.0
51	34.23137893941371	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	4.0
13	13.0
14	16.0
15	27.0
16	89.0
17	201.0
18	435.0
19	853.0
20	1555.0
21	2460.0
22	3878.0
23	5689.0
24	8405.0
25	12300.0
26	16234.0
27	18936.0
28	20586.0
29	22985.0
30	26793.0
31	32717.0
32	40195.0
33	52821.0
34	85670.0
35	119756.0
36	102332.0
37	135473.0
38	193867.0
39	314214.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.49626951091331	23.105073186181478	26.148459762984917	14.250197539920295
2	34.659455946751606	24.667094977521884	27.030927818133634	13.642521257592877
3	30.713170394694195	25.278463594808088	30.528635698643107	13.47973031185461
4	26.788218333509743	28.208864721872818	29.47476124951897	15.528155695098466
5	23.37108540698967	31.742593381202404	29.173466716882423	15.712854494925502
6	22.055793642783826	40.89810714499635	26.86764456106753	10.178454651152295
7	87.11375099385349	3.6572988026167987	7.459173031841876	1.7697771716878319
8	84.0911235447072	6.224045962185567	7.133673192253317	2.551157300853914
9	81.50255058293764	5.2134128580231565	9.724297451222204	3.559739107817001
10	40.91632266412634	28.869546523830742	16.082005938915653	14.13212487312727
11	32.7811241272756	27.00385069510257	23.486368309591622	16.728656868030207
12	28.452394725376433	23.705118642927413	29.10454313098517	18.73794350071098
13	23.694041638051072	27.091235955793724	30.926423251535805	18.2882991546194
14	21.328239552127975	28.679760506949386	31.222138255790195	18.769861685132444
15	21.091355751550164	23.963335934378183	35.65170942800808	19.293598886063567
16	24.032997987267187	22.437088766193966	33.77624942461114	19.75366382192771
17	23.697241661682014	23.303146443748098	29.25223652933642	23.747375365233466
18	25.470875272104575	23.013257123539784	30.457496711770588	21.058370892585053
19	27.933908845275575	25.11797010193306	26.623129934908235	20.324991117883126
20	28.994347445437548	24.87066571158269	26.351948445157745	19.783038397822015
21	27.92028823187207	25.13314970120805	27.3521609595476	19.59440110737228
22	26.461077456161732	23.284520665178245	27.14563635751977	23.10876552114026
23	24.346354147435754	26.820792933034994	26.71100750692723	22.12184541260202
24	23.56456375882981	24.92350712743725	31.040065116378297	20.471863997354646
25	23.792996214946406	25.387592605812063	29.194143792651595	21.625267386589933
26	22.137189115652955	26.926886024183972	27.99692469523877	22.939000164924295
27	22.732229407232545	25.681256312867127	28.852725886796293	22.73378839310403
28	22.13390704013404	26.617058095198242	30.21462312337076	21.03441174129696
29	22.903799904983913	25.134708687079538	30.013924205389003	21.947567202547546
30	23.726862393240236	25.587717160578006	29.839317787782637	20.84610265839912
31	26.276706863886584	25.536762938146822	27.07761534239022	21.10891485557637
32	26.207783277989332	25.71727709168724	27.046845884400376	21.028093745923044
33	25.436331427267977	25.567204188584775	26.20368068359069	22.79278370055656
34	22.83364554076707	26.72585889865033	28.555123689118524	21.885371871464077
35	23.521568569532	26.70854595028804	28.016042785136463	21.75384269504349
36	25.859801221096195	27.56204968898232	25.57540937738207	21.002739712539416
37	24.026351784341383	27.683814690734128	25.99379195415597	22.296041570768523
38	23.91156119306727	28.882756877794378	25.731307964530608	21.474373964607736
39	24.40731870019963	26.816444182972425	25.348864114688848	23.427373002139092
40	25.1499703382425	25.655574071931607	26.986373642964338	22.208081946861558
41	22.36964211428023	25.75321581861938	27.61226544442174	24.26487662267865
42	24.57150452803344	26.41947714895946	25.83362666883284	23.175391654174266
43	23.620687250203286	25.46070083799593	27.562706104086104	23.355905807714684
44	23.145524766952125	26.59260663258231	27.673065893409678	22.588802707055887
45	22.453909403228415	27.252960226988343	26.691479157589676	23.601651212193566
46	23.73285218106226	26.362533138706258	27.299729803132905	22.60488487709858
47	22.87130735734664	26.34940483663059	28.389132719749316	22.39015508627346
48	23.63717967968584	25.932253038176285	28.231511042953343	22.199056239184536
49	23.482840078408785	25.62185074597474	28.22749050044267	22.667818675173805
50	22.298421075519737	27.019194398153505	28.397830219874443	22.284554306452314
51	22.347077845087675	27.27092959045441	27.00179939790325	23.380193166554665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	965.0
1	1516.0
2	2067.0
3	7629.0
4	13191.0
5	9283.0
6	5375.0
7	5512.5
8	5650.0
9	5830.0
10	6010.0
11	6210.5
12	6411.0
13	6449.0
14	6487.0
15	6392.0
16	6297.0
17	6076.0
18	5855.0
19	5825.5
20	5796.0
21	5899.5
22	6003.0
23	6256.5
24	6510.0
25	6784.5
26	8723.5
27	10388.0
28	12383.0
29	14378.0
30	16108.0
31	17838.0
32	20602.0
33	23366.0
34	26289.5
35	29213.0
36	32647.5
37	36082.0
38	38882.0
39	41682.0
40	48280.0
41	54878.0
42	61795.0
43	68712.0
44	71352.5
45	73993.0
46	79587.5
47	85182.0
48	88440.0
49	91698.0
50	90177.5
51	88657.0
52	85856.0
53	83055.0
54	76844.5
55	70634.0
56	67389.0
57	64144.0
58	62544.0
59	60944.0
60	56863.5
61	52783.0
62	48952.5
63	45122.0
64	40904.5
65	36687.0
66	32066.0
67	27445.0
68	23747.0
69	20049.0
70	16841.0
71	13633.0
72	11806.0
73	9979.0
74	8419.0
75	5474.0
76	4089.0
77	2893.0
78	1697.0
79	1375.0
80	1053.0
81	752.0
82	451.0
83	342.0
84	233.0
85	150.0
86	67.0
87	49.0
88	31.0
89	28.0
90	25.0
91	17.5
92	10.0
93	8.0
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1218741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.56526570036445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.11932871066166	20.4311898944204
2	6.057628671095418	3.339602876657816
3	2.830229255068544	2.340480642267269
4	1.910754690249842	2.106818428998179
5	1.3851506068715636	1.9091022256717856
6	1.1751375544534566	1.9435787353791616
7	0.9606297690188201	1.8536010375878451
8	0.8576109213387442	1.891221833138947
9	0.7631805966855347	1.8933548332499501
>10	9.693043990831967	56.308801130920706
>50	0.21792289996627381	3.6880320660538093
>100	0.02754593789863778	1.4345917660231267
>500	0.0015303298828427195	0.24205150412320398
>1k	0.0	0.0
>5k	3.0606597656854386E-4	0.6175730255077913
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7320	0.6006198199617474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.205188797291631E-5	0.0	0.0	0.027487382470926965	0.0
2	8.205188797291631E-5	0.0	0.0	0.09280068529736835	0.0
3	8.205188797291631E-5	0.0	0.0	0.1524524078536785	0.0
4	8.205188797291631E-5	0.0	0.0	0.26084295186590095	0.0
5	8.205188797291631E-5	0.0	0.0	0.441193001630371	0.0
6	8.205188797291631E-5	0.0	0.0	0.9326017587001668	0.0
7	8.205188797291631E-5	0.0	0.0	1.159803436497172	0.0
8	8.205188797291631E-5	0.0	0.0	1.7430282562086612	0.0
9	8.205188797291631E-5	0.0	0.0	2.047030501148316	0.0
10	8.205188797291631E-5	0.0	0.0	2.477392653566262	0.0
11	8.205188797291631E-5	0.0	0.0	2.7831179881533483	0.0
12	8.205188797291631E-5	0.0	0.0	3.056104619439241	0.0
13	8.205188797291631E-5	0.0	0.0	3.218649409513588	0.0
14	8.205188797291631E-5	0.0	0.0	3.3040654248933943	0.0
15	8.205188797291631E-5	0.0	0.0	3.382424977907529	0.0
16	8.205188797291631E-5	0.0	0.0	3.495492479534208	0.0
17	8.205188797291631E-5	0.0	0.0	3.608970240600751	0.0
18	8.205188797291631E-5	0.0	0.0	3.774879158081988	0.0
19	8.205188797291631E-5	0.0	0.0	3.8644798197484125	0.0
20	8.205188797291631E-5	0.0	0.0	3.9661421089468556	0.0
21	8.205188797291631E-5	0.0	0.0	4.090368667337851	0.0
22	8.205188797291631E-5	0.0	0.0	4.21722088614398	0.0
23	8.205188797291631E-5	0.0	0.0	4.366391218478742	0.0
24	8.205188797291631E-5	0.0	0.0	4.4862690268071725	0.0
25	8.205188797291631E-5	0.0	0.0	4.597367283122501	0.0
26	8.205188797291631E-5	0.0	0.0	4.704855256367021	0.0
27	8.205188797291631E-5	0.0	0.0	4.8140663192589725	0.0
28	8.205188797291631E-5	0.0	0.0	4.922456863271195	0.0
29	8.205188797291631E-5	0.0	0.0	5.044385968798949	0.0
30	8.205188797291631E-5	0.0	0.0	5.20504356544992	0.0
31	8.205188797291631E-5	0.0	0.0	5.330336798384563	0.0
32	8.205188797291631E-5	0.0	0.0	5.480902012814864	0.0
33	8.205188797291631E-5	0.0	0.0	5.62145689691247	0.0
34	8.205188797291631E-5	0.0	0.0	5.757006615843728	0.0
35	8.205188797291631E-5	0.0	0.0	5.902976924547545	0.0
36	8.205188797291631E-5	0.0	0.0	6.0356548273997515	0.0
37	8.205188797291631E-5	0.0	0.0	6.174322518073979	0.0
38	8.205188797291631E-5	0.0	0.0	6.338180138355893	0.0
39	8.205188797291631E-5	0.0	0.0	6.581135778643699	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	35	1.2120108E-7	45.000004	1
CTATACG	20	7.033454E-4	45.0	1
CGGTCTA	95	0.0	42.63158	31
AGGGCGA	1525	0.0	39.098362	6
CGTTTTT	5020	0.0	38.90438	1
GGGCGAT	3050	0.0	37.69672	7
TAGTACG	30	1.1399625E-4	37.499996	1
TTACACG	720	0.0	36.875	34
TCACGAC	110	0.0	36.81818	25
CTCACGA	110	0.0	36.81818	24
CACGACG	110	0.0	36.81818	26
TCAAGCG	850	0.0	36.794117	17
GGCGATT	710	0.0	36.443665	8
ATACTAT	555	0.0	36.08108	45
CGTATAA	25	0.0021071585	36.0	26
GCGAATA	25	0.0021071585	36.0	25
TTATGCG	50	4.879439E-8	36.0	1
CATAGCG	50	4.879439E-8	36.0	1
ACTAACG	25	0.0021071585	36.0	1
TATAGCG	50	4.879439E-8	36.0	1
>>END_MODULE