##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547108_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	954048
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.478164620648016	31.0	31.0	33.0	30.0	34.0
2	31.848305326356744	31.0	31.0	34.0	30.0	34.0
3	31.951807456228618	33.0	31.0	34.0	30.0	34.0
4	35.67644290434024	37.0	35.0	37.0	33.0	37.0
5	35.60034820050983	37.0	35.0	37.0	33.0	37.0
6	35.656296119272824	37.0	35.0	37.0	33.0	37.0
7	36.0408753018716	37.0	35.0	37.0	35.0	37.0
8	34.73557619742403	37.0	35.0	37.0	33.0	37.0
9	37.078415341785735	39.0	37.0	39.0	34.0	39.0
10	36.898828989736366	39.0	37.0	39.0	32.0	39.0
11	36.855841634802445	39.0	37.0	39.0	33.0	39.0
12	36.372677265714096	38.0	35.0	39.0	32.0	39.0
13	36.149241966861204	38.0	35.0	39.0	32.0	39.0
14	37.24047846649225	39.0	35.0	41.0	32.0	41.0
15	37.48130597202656	39.0	35.0	41.0	33.0	41.0
16	37.58805007714496	39.0	35.0	41.0	33.0	41.0
17	37.506781629435835	39.0	35.0	41.0	33.0	41.0
18	37.47525596196418	39.0	35.0	41.0	33.0	41.0
19	37.41093005802643	39.0	35.0	41.0	33.0	41.0
20	37.27056814751459	39.0	35.0	41.0	32.0	41.0
21	37.164852292547124	39.0	35.0	41.0	32.0	41.0
22	37.07416922418998	39.0	35.0	41.0	32.0	41.0
23	37.043344779298316	39.0	35.0	41.0	32.0	41.0
24	36.9119048517475	38.0	35.0	41.0	32.0	41.0
25	36.83563824880928	38.0	35.0	41.0	32.0	41.0
26	36.72468576004562	38.0	35.0	41.0	32.0	41.0
27	36.63117578989736	38.0	35.0	41.0	31.0	41.0
28	36.67055221540216	38.0	35.0	41.0	31.0	41.0
29	36.66782279298316	38.0	35.0	41.0	31.0	41.0
30	36.62934464513316	38.0	35.0	41.0	31.0	41.0
31	36.533003580532636	38.0	35.0	41.0	31.0	41.0
32	36.43031377876166	38.0	35.0	41.0	31.0	41.0
33	36.30411153317233	38.0	35.0	41.0	30.0	41.0
34	36.1959335379352	38.0	35.0	41.0	30.0	41.0
35	36.07308227678272	38.0	35.0	41.0	30.0	41.0
36	35.918456932984505	38.0	35.0	41.0	30.0	41.0
37	35.863744801100154	38.0	35.0	41.0	30.0	41.0
38	35.815744071577114	38.0	35.0	41.0	30.0	41.0
39	35.761399845710066	38.0	35.0	41.0	29.0	41.0
40	35.61582331287315	38.0	35.0	41.0	29.0	41.0
41	35.563325954249684	38.0	35.0	40.0	29.0	41.0
42	35.51126672871805	38.0	35.0	40.0	28.0	41.0
43	35.44743555879788	38.0	35.0	40.0	28.0	41.0
44	35.342820277386465	38.0	34.0	40.0	28.0	41.0
45	35.23038358657007	38.0	34.0	40.0	27.0	41.0
46	35.18977137418663	37.0	34.0	40.0	27.0	41.0
47	35.13764611424163	37.0	34.0	40.0	27.0	41.0
48	35.09349424766888	37.0	34.0	40.0	27.0	41.0
49	35.055073748909905	37.0	34.0	40.0	27.0	41.0
50	34.9373532568592	37.0	34.0	40.0	27.0	41.0
51	34.58640340947206	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	5.0
13	3.0
14	7.0
15	23.0
16	65.0
17	171.0
18	367.0
19	699.0
20	1116.0
21	1924.0
22	2849.0
23	4265.0
24	6439.0
25	9589.0
26	12557.0
27	14416.0
28	15643.0
29	17506.0
30	20904.0
31	25353.0
32	32590.0
33	41889.0
34	73520.0
35	130195.0
36	66713.0
37	84496.0
38	129793.0
39	260706.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.65899409673308	25.108485107667537	29.61423324612598	13.6182875494734
2	30.107499832293556	27.456060910981417	29.74032753069028	12.696111726034747
3	28.07542178171329	25.67596179647146	31.92103541960153	14.327581002213725
4	24.748440330046286	28.983657006775342	31.55438720064399	14.71351546253438
5	22.347722546454683	32.76700962635004	30.61082880525927	14.274439021936002
6	20.157895619507613	40.859579392231836	28.275306902797343	10.707218085463206
7	87.96894915140538	3.9144780975380695	6.56004729321795	1.5565254578385992
8	86.16023512443817	5.848552693365533	5.438720064399275	2.552492117797008
9	84.41095206949754	4.514133460790233	8.12380509156772	2.951109378144496
10	41.77200727845978	25.475971858858255	16.991388273965253	15.76063258871671
11	37.135657744683705	25.28478650969343	22.706614342255314	14.872941403367545
12	34.073652478701284	23.07504444220836	26.410201583148858	16.441101495941503
13	19.853718051921916	38.35352099684712	26.507471322197624	15.285289629033342
14	16.126966358086804	38.96952773864628	28.066512376735766	16.83699352653116
15	14.54538974978198	25.6941998725431	43.4898453746562	16.270565003018717
16	15.792496813577515	21.04558680485678	42.94228382639029	20.21963255517542
17	15.920792245253907	23.06299054135641	29.40229422418998	31.613922989199704
18	19.702991883007982	25.30291976923593	34.070403166297716	20.923685181458378
19	24.572872643724423	28.767839773260885	26.060743274971486	20.598544308043202
20	26.89560692963037	25.47586704232911	26.568264909103107	21.06026111893741
21	20.507353927684978	30.246067283826388	28.54384685047293	20.7027319380157
22	21.516317837257663	26.79812755752331	26.379804789696117	25.30574981552291
23	18.51101831354397	30.868048567786943	26.19648068021735	24.424452438451734
24	18.35148755618166	26.743518145837523	37.2500125779835	17.654981719997316
25	17.989136814919167	28.559988595961627	32.865956429865165	20.58491815925404
26	17.28466492251962	33.20168377272422	27.457737975447778	22.055913329308378
27	17.313908734151738	32.503081605956936	28.846661803179714	21.336347856711612
28	15.74899795398135	29.026736600254914	36.14000553431274	19.084259911450996
29	15.850145904608572	25.304596833702288	34.842481719997316	24.002775541691822
30	18.430414402629637	30.332750553431275	32.1780455490709	19.058789494868183
31	24.39699050781512	29.34716072985846	26.160214161132355	20.09563460119407
32	25.078193130743948	27.117503521835378	27.968089655866375	19.836213691554303
33	23.47470986784732	28.62308814650835	26.15989971154491	21.74230227409942
34	19.316742973099885	26.992876668679145	29.011328570470248	24.679051787750723
35	19.358774401287985	27.643368048567783	30.013898671764945	22.983958878379283
36	27.003358321593886	27.104925538337692	26.232852015831487	19.658864124236935
37	19.746385926075	31.77303448044543	29.15534648151875	19.325233111960824
38	20.72914570336084	31.053469007848662	27.627226303079087	20.59015898571141
39	20.52978466492252	31.651237673576173	27.03910075803314	20.77987690346817
40	23.69199453276984	26.036530656738442	27.879414872207686	22.392059938284028
41	18.189546018648954	25.515487690346816	29.002628798551015	27.29233749245321
42	21.221259307707786	27.44998155229087	26.299305695310927	25.029453444690414
43	20.624433990742606	27.332796672704095	28.270170892869125	23.772598443684174
44	20.010942845642987	29.483631850808344	28.642479204400615	21.86294609914805
45	17.693763835781848	34.44407409270812	24.958911920574227	22.903250150935804
46	21.820809854430802	30.09282551821292	27.939684376467433	20.146680250888842
47	21.213083618434293	27.91253689541826	28.689541825987792	22.184837660159655
48	21.376597403904206	25.3519739048769	30.604539813510435	22.66688887770846
49	20.591207151002884	24.85587727242235	31.56099064198028	22.991924934594486
50	19.551741631448312	29.59589035352519	29.640122928825384	21.212245086201115
51	18.272141443617095	29.981929630374992	28.176884181927953	23.56904474407996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1093.0
1	1197.0
2	1301.0
3	4321.5
4	7342.0
5	5387.0
6	3432.0
7	3720.0
8	4008.0
9	4354.0
10	4700.0
11	4877.5
12	5055.0
13	5095.5
14	5136.0
15	4971.5
16	4807.0
17	4637.5
18	4468.0
19	4533.0
20	4598.0
21	4314.5
22	4031.0
23	4251.5
24	4472.0
25	5562.0
26	7916.0
27	9180.0
28	11222.0
29	13264.0
30	15127.0
31	16990.0
32	19912.0
33	22834.0
34	25914.0
35	28994.0
36	32712.0
37	36430.0
38	41356.5
39	46283.0
40	55600.0
41	64917.0
42	73934.0
43	82951.0
44	84568.0
45	86185.0
46	89460.0
47	92735.0
48	93091.0
49	93447.0
50	86319.5
51	79192.0
52	70047.0
53	60902.0
54	52729.5
55	44557.0
56	38679.0
57	32801.0
58	29420.5
59	26040.0
60	22573.5
61	19107.0
62	15927.0
63	12747.0
64	10650.5
65	8554.0
66	6940.5
67	5327.0
68	4334.0
69	3341.0
70	2795.5
71	2250.0
72	1925.5
73	1601.0
74	1237.5
75	692.0
76	510.0
77	483.5
78	457.0
79	342.0
80	227.0
81	186.5
82	146.0
83	113.0
84	80.0
85	47.0
86	14.0
87	10.0
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	954048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.235691786477954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26991787981783	20.534654416478404
2	6.87870430496122	3.6093513207056325
3	2.6649740409827825	2.0975231267456684
4	1.5219196418227448	1.5971445858659383
5	1.0454975243577111	1.371467540628732
6	0.7751615879905925	1.22021403043428
7	0.6093544202282661	1.119078433048573
8	0.5216711232941157	1.0949122243720129
9	0.4503619104534725	1.0634000647524022
>10	5.7482450121977005	36.18216713297594
>50	1.397368135255943	24.607495685142585
>100	0.11229952918334395	4.175481255400391
>500	0.00370218228076958	0.6847616584521965
>1k	8.227071735043511E-4	0.6423485249972377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4407	0.4619264439525056	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1545	0.16194153753270277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047272254645468574	0.0
2	0.0	0.0	0.0	0.19464429462668545	0.0
3	0.0	0.0	0.0	0.3280757362313007	0.0
4	0.0	0.0	0.0	0.6901120279063527	0.0
5	0.0	0.0	0.0	1.1704861809887972	0.0
6	0.0	0.0	0.0	2.0467523646608976	0.0
7	0.0	0.0	0.0	2.4305904943986048	0.0
8	0.0	0.0	0.0	3.4141888039176225	0.0
9	0.0	0.0	0.0	3.8949822231166564	0.0
10	0.0	0.0	0.0	4.660666968538271	0.0
11	0.0	0.0	0.0	5.369121889045415	0.0
12	0.0	0.0	0.0	5.938799724961427	0.0
13	0.0	0.0	0.0	6.211532333802912	0.0
14	0.0	0.0	0.0	6.309535788555712	0.0
15	0.0	0.0	0.0	6.41927869457302	0.0
16	0.0	0.0	0.0	6.675659924867512	0.0
17	0.0	0.0	0.0	6.936862715502784	0.0
18	0.0	0.0	0.0	7.331287314684377	0.0
19	0.0	0.0	0.0	7.496897430737238	0.0
20	0.0	0.0	0.0	7.699717414637418	0.0
21	0.0	0.0	0.0	7.962073187093312	0.0
22	0.0	0.0	0.0	8.21939776615013	0.0
23	0.0	0.0	0.0	8.517600791574429	0.0
24	0.0	0.0	0.0	8.741803347420674	0.0
25	0.0	0.0	0.0	8.929529751123633	0.0
26	0.0	0.0	0.0	9.099542161400684	0.0
27	0.0	0.0	0.0	9.274376132018515	0.0
28	0.0	0.0	0.0	9.447637854699135	0.0
29	0.0	0.0	0.0	9.642491782384115	0.0
30	0.0	0.0	0.0	9.897824847387133	0.0
31	0.0	0.0	0.0	10.108715704031663	0.0
32	0.0	0.0	0.0	10.289419400281746	0.0
33	0.0	0.0	0.0	10.486474475078822	0.0
34	0.0	0.0	0.0	10.698413497014826	0.0
35	0.0	0.0	0.0	10.954899543838465	0.0
36	0.0	0.0	0.0	11.166419299657878	0.0
37	0.0	0.0	0.0	11.366199604212786	0.0
38	0.0	0.0	0.0	11.575727845978399	0.0
39	0.0	0.0	0.0	11.79144026296371	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.0326845E-4	45.0	15
CGGGTAC	85	0.0	45.0	6
GACCGTA	25	3.890306E-5	45.0	9
GTTAGCG	20	7.0326845E-4	45.0	1
CTCGGTT	20	7.0326845E-4	45.0	19
CTACGTT	20	7.0326845E-4	45.0	30
TGATTCG	55	1.8189894E-12	45.0	15
TGTTACG	35	1.2116288E-7	45.0	1
GCTAGCG	25	3.890306E-5	45.0	1
CCGACGG	20	7.0326845E-4	45.0	2
CGTAACA	20	7.0326845E-4	45.0	33
CCGAAAC	25	3.890306E-5	45.0	44
ACCAACG	20	7.0326845E-4	45.0	21
GCTCGTC	25	3.890306E-5	45.0	9
TCGGTCA	20	7.0326845E-4	45.0	22
GCGTAAC	70	0.0	45.0	32
TCGCGAG	20	7.0326845E-4	45.0	1
ACGCGTA	20	7.0326845E-4	45.0	23
AATACGG	100	0.0	42.75	2
CGCATCG	75	0.0	42.000004	21
>>END_MODULE