FastQCFastQC Report
Thu 26 May 2016
SRR1547107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547107_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1157258
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189941.641293471291622No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT15030.12987596542862526No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC12490.10792753214927009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG351.211938E-745.0000041
CGTAACT351.211938E-745.00000433
CGCGTAA351.211938E-745.00000431
GCGTAAC351.211938E-745.00000432
CGTTTTT113500.043.7709241
TAGCACG403.4582445E-739.3751
GCGATCG800.039.3759
GTTTTTT125950.038.6939282
ACGCTAG356.248325E-638.571431
TTCGTAG659.094947E-1238.076921
CTATCGA301.1399268E-437.49999641
TATTACG301.1399268E-437.4999961
TAAGGGA12600.037.1428574
TAGGGAT13700.037.1167875
ATAACGG850.037.0588262
GACCGAT7650.037.0588239
TCAGACG1950.036.92307722
CATACGA1650.036.8181818
CGACCCG1600.036.562532
GCGTAAG1050.036.428571
Produced by FastQC (version 0.11.5)