##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547107_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1157258
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.411909012510606	31.0	31.0	33.0	30.0	34.0
2	31.770877367017555	31.0	31.0	34.0	30.0	34.0
3	31.743781421256106	31.0	31.0	34.0	30.0	34.0
4	35.58456886882614	37.0	35.0	37.0	33.0	37.0
5	35.53843568158526	37.0	35.0	37.0	33.0	37.0
6	35.60151150391702	37.0	35.0	37.0	33.0	37.0
7	36.02025218231371	37.0	35.0	37.0	35.0	37.0
8	34.727081601509774	37.0	35.0	37.0	33.0	37.0
9	37.02634589693914	39.0	37.0	39.0	34.0	39.0
10	36.8763646481597	39.0	37.0	39.0	32.0	39.0
11	36.7721026771904	39.0	37.0	39.0	32.0	39.0
12	36.55254835136158	39.0	35.0	39.0	32.0	39.0
13	36.5027487388292	39.0	35.0	39.0	32.0	39.0
14	37.615664786936016	40.0	36.0	41.0	32.0	41.0
15	37.772278955945865	40.0	36.0	41.0	33.0	41.0
16	37.795020643624845	40.0	36.0	41.0	33.0	41.0
17	37.70757687568373	40.0	36.0	41.0	33.0	41.0
18	37.55181817710485	39.0	36.0	41.0	33.0	41.0
19	37.39367712299245	39.0	36.0	41.0	32.0	41.0
20	37.169859270793545	39.0	35.0	41.0	32.0	41.0
21	37.08172680594993	39.0	35.0	41.0	32.0	41.0
22	36.992160780050774	38.0	35.0	41.0	32.0	41.0
23	36.90379500508962	38.0	35.0	41.0	32.0	41.0
24	36.760082885579536	38.0	35.0	41.0	31.0	41.0
25	36.663225486451594	38.0	35.0	40.0	31.0	41.0
26	36.54802559152756	38.0	35.0	40.0	31.0	41.0
27	36.43595032395542	38.0	35.0	40.0	31.0	41.0
28	36.4234120654167	38.0	35.0	40.0	31.0	41.0
29	36.37567681536874	38.0	35.0	40.0	31.0	41.0
30	36.27102945064973	38.0	35.0	40.0	31.0	41.0
31	36.0798205758785	38.0	35.0	40.0	30.0	41.0
32	35.81447093042347	38.0	35.0	40.0	29.0	41.0
33	35.45194502867986	38.0	35.0	40.0	27.0	41.0
34	35.105544312504215	38.0	34.0	40.0	24.0	41.0
35	34.84089718973643	38.0	34.0	40.0	23.0	41.0
36	34.669442769028166	38.0	34.0	40.0	22.0	41.0
37	34.60768471680472	38.0	34.0	40.0	22.0	41.0
38	34.51278107388327	38.0	34.0	40.0	21.0	41.0
39	34.422488330173564	38.0	34.0	40.0	21.0	41.0
40	34.30631026097897	38.0	34.0	40.0	20.0	41.0
41	34.21934175438839	38.0	34.0	40.0	20.0	41.0
42	34.14480176417013	37.0	33.0	40.0	19.0	41.0
43	34.05602380800133	37.0	33.0	40.0	18.0	41.0
44	33.94834168353125	37.0	33.0	40.0	18.0	41.0
45	33.837648994433394	37.0	33.0	40.0	18.0	41.0
46	33.767033798859025	37.0	33.0	40.0	18.0	41.0
47	33.698572833369916	37.0	33.0	40.0	18.0	41.0
48	33.59957157349528	36.0	33.0	40.0	18.0	41.0
49	33.52861937441781	36.0	33.0	40.0	18.0	41.0
50	33.43239709727649	36.0	33.0	40.0	18.0	41.0
51	33.072037523179795	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	6.0
14	13.0
15	48.0
16	96.0
17	250.0
18	607.0
19	1265.0
20	2178.0
21	3420.0
22	5199.0
23	8002.0
24	12026.0
25	19018.0
26	26474.0
27	29149.0
28	27106.0
29	27010.0
30	28618.0
31	32960.0
32	39733.0
33	50573.0
34	79703.0
35	118651.0
36	92139.0
37	112848.0
38	160871.0
39	279113.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.111313121188186	24.191666853890837	27.160062838191656	19.53695718672932
2	36.696484275762195	23.712776234858605	27.842451726408456	11.748287762970747
3	26.923641919088055	23.9191260721464	36.6215658046866	12.535666204078952
4	23.344232660305654	26.706922743243076	36.37667659242796	13.572168004023302
5	21.08492661100636	30.443773125785263	35.094507879833195	13.376792383375186
6	19.657155102837915	38.94827255460753	31.67616901330559	9.718403329248966
7	82.13285196559454	3.200150701053698	13.070724073629217	1.5962732597225509
8	80.04878773791151	5.463345252311931	12.205316359878264	2.282550649898294
9	77.29849350792996	4.778450440610477	14.881383408021376	3.041672643438196
10	38.237627218822425	27.4003722592542	21.42832453955816	12.933675982365212
11	29.71083371210223	23.980305169633738	30.42605883908342	15.882802279180616
12	26.749955498255357	21.73681236163414	33.5257997784418	17.987432361668702
13	21.364985163204746	26.7494370313275	35.31425144609067	16.57132635937708
14	18.905550879751964	29.719388416411896	34.27187368763059	17.10318701620555
15	18.010158495339844	24.221478702242717	41.184766059081035	16.583596743336404
16	19.710125140634155	22.55832320882638	40.12294579082625	17.60860585971322
17	20.23809729550368	22.921941347564676	33.381061094414555	23.458900262517087
18	21.243491079776504	24.379956759858217	35.822176213083	18.554375947282285
19	24.009166495284543	26.123128982474093	31.51881430070045	18.348890221540916
20	25.198875272411165	25.172433459090364	30.89034597298096	18.738345295517508
21	22.870785944015942	26.938591048841314	32.562747459944106	17.627875547198638
22	22.171978936416945	24.316530972350158	32.29331747976683	21.21817261146607
23	20.02129170850407	27.950292847403084	31.32283380196983	20.70558164212302
24	20.137169066880507	25.938122700383147	35.91411768162329	18.01059055111306
25	20.5919509737673	26.064887864244618	34.23307507919582	19.110086082792254
26	18.876257498327945	28.94315701425266	32.112718166562686	20.067867320856713
27	19.664240817518653	27.629361819058502	33.0824241439679	19.623973219454953
28	18.891638683854424	27.2595220771859	35.550326720575704	18.298512518383973
29	19.488394117819883	25.516004209951454	34.93663470030019	20.05896697192847
30	20.946927997041282	26.987845406987898	33.511628349080326	18.553598246890495
31	23.737921880859755	26.644015422662882	30.57459961391496	19.0434630825624
32	25.126030669047005	26.555616811462958	30.17676265793799	18.14158986155205
33	23.975984611901584	27.227895594586514	29.406580036603764	19.38953975690814
34	21.37578655753514	27.786111653581138	30.580129927812123	20.257971861071603
35	21.25074961676653	27.354919991911913	31.002853296326315	20.39147709499524
36	24.541286385576942	27.97967263998175	28.147915158071925	19.331125816369383
37	21.4592597329204	29.759137547547738	29.71748737100975	19.064115348522108
38	21.49373778362301	30.512642816035836	27.879262878286433	20.11435652205472
39	20.975702911537443	30.113423281584573	28.174356971392722	20.736516835485258
40	22.047546873730838	27.682331856854738	28.97677095340883	21.293350316005593
41	19.74641782558427	27.474426618783365	29.12790406287967	23.651251492752696
42	21.248762160209736	28.754089407893485	27.57777435973655	22.419374072160227
43	21.034548907849416	27.93326984993839	29.22399326684283	21.808187975369364
44	21.50808203529377	28.88482948486854	28.80213400987507	20.80495446996262
45	20.123688926756177	29.89592640534781	28.04266637171659	21.93771829617942
46	21.814063933885098	28.9753019637799	28.29766568906847	20.91296841326653
47	21.582395628286864	27.60784544155236	29.183898491088417	21.62586043907236
48	21.839295991040892	27.01601544340156	29.69510688195718	21.44958168360037
49	21.24418236901365	26.347452339927656	30.323488798522025	22.08487649253667
50	20.578557244797615	28.152408538113367	29.861534765799846	21.40749945128917
51	19.628639421805683	29.0123723491218	28.956291509758415	22.402696719314104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	969.0
1	1243.0
2	1517.0
3	15229.5
4	28942.0
5	19655.0
6	10368.0
7	10370.5
8	10373.0
9	11452.0
10	12531.0
11	13109.5
12	13688.0
13	13750.0
14	13812.0
15	13555.0
16	13298.0
17	12592.0
18	11886.0
19	11129.5
20	10373.0
21	9916.0
22	9459.0
23	9235.0
24	9011.0
25	9226.0
26	10625.5
27	11810.0
28	13188.0
29	14566.0
30	15920.0
31	17274.0
32	20221.0
33	23168.0
34	26597.0
35	30026.0
36	32101.0
37	34176.0
38	37878.5
39	41581.0
40	48889.5
41	56198.0
42	65239.5
43	74281.0
44	79002.5
45	83724.0
46	89477.5
47	95231.0
48	96435.0
49	97639.0
50	93212.0
51	88785.0
52	79812.5
53	70840.0
54	64520.5
55	58201.0
56	53851.0
57	49501.0
58	45406.5
59	41312.0
60	37051.5
61	32791.0
62	28460.0
63	24129.0
64	21951.5
65	19774.0
66	16653.5
67	13533.0
68	11060.5
69	8588.0
70	6948.0
71	5308.0
72	4654.5
73	4001.0
74	3283.0
75	2007.0
76	1449.0
77	1032.0
78	615.0
79	456.5
80	298.0
81	220.5
82	143.0
83	95.0
84	47.0
85	29.0
86	11.0
87	14.5
88	18.0
89	10.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1157258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.88015038337153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9107061412901	27.59567702440785
2	7.465419712335494	5.357207639071675
3	3.253524105585432	3.5021080255298895
4	1.9695346692164901	2.8266880046700624
5	1.4413340633675318	2.585764147315149
6	1.0827332478351277	2.330917905444039
7	0.8349483295452211	2.097065013849917
8	0.7235261416548899	2.0768181415102416
9	0.5678533047645938	1.8337195773583317
>10	5.514137865538468	41.243714539945124
>50	0.21332231363204282	4.7178224246256475
>100	0.01999751102636388	1.307646574718946
>500	0.0022219456561542363	0.5985481557153712
>1k	4.937657013676082E-4	0.24377749364047677
>5k	0.0	0.0
>10k+	2.468828506838041E-4	1.6825253321973894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18994	1.641293471291622	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT	1503	0.12987596542862526	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	1249	0.10792753214927009	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.592334639293917E-4	0.0	0.0	0.06454913251841854	0.0
2	2.592334639293917E-4	0.0	0.0	0.19036377367881666	0.0
3	2.592334639293917E-4	0.0	0.0	0.3698397418725988	0.0
4	2.592334639293917E-4	0.0	0.0	0.580423725737908	0.0
5	2.592334639293917E-4	0.0	0.0	0.9909631214474214	0.0
6	2.592334639293917E-4	0.0	0.0	1.8281143876300703	0.0
7	2.592334639293917E-4	0.0	0.0	2.285402218001517	0.0
8	3.456446185725223E-4	0.0	0.0	3.192546519445102	0.0
9	3.456446185725223E-4	0.0	0.0	3.700471286437424	0.0
10	3.456446185725223E-4	0.0	0.0	4.354517315931279	0.0
11	3.456446185725223E-4	0.0	0.0	4.874453233418995	0.0
12	3.456446185725223E-4	0.0	0.0	5.30573130624286	0.0
13	3.456446185725223E-4	0.0	0.0	5.5621996132236715	0.0
14	3.456446185725223E-4	0.0	0.0	5.7091849872716365	0.0
15	3.456446185725223E-4	0.0	0.0	5.838196841153831	0.0
16	3.456446185725223E-4	0.0	0.0	6.031066538317298	0.0
17	3.456446185725223E-4	0.0	0.0	6.266796168183759	0.0
18	3.456446185725223E-4	0.0	0.0	6.5688031536614995	0.0
19	3.456446185725223E-4	0.0	0.0	6.736440793669173	0.0
20	3.456446185725223E-4	0.0	0.0	6.911336970666869	0.0
21	3.456446185725223E-4	0.0	0.0	7.119501442202171	0.0
22	3.456446185725223E-4	0.0	0.0	7.348058946233252	0.0
23	3.456446185725223E-4	0.0	0.0	7.608156521709074	0.0
24	4.3205577321565284E-4	0.0	0.0	7.819518205966172	0.0
25	4.3205577321565284E-4	0.0	0.0	7.996229017211374	0.0
26	4.3205577321565284E-4	0.0	0.0	8.1625704898994	0.0
27	4.3205577321565284E-4	0.0	0.0	8.3518973297225	0.0
28	4.3205577321565284E-4	0.0	0.0	8.532237409462713	0.0
29	4.3205577321565284E-4	0.0	0.0	8.729686897822265	0.0
30	4.3205577321565284E-4	0.0	0.0	8.997215832597398	0.0
31	4.3205577321565284E-4	0.0	0.0	9.203392847575907	0.0
32	4.3205577321565284E-4	0.0	0.0	9.406804705605838	0.0
33	4.3205577321565284E-4	0.0	0.0	9.616956633697932	0.0
34	4.3205577321565284E-4	0.0	0.0	9.831515530676825	0.0
35	4.3205577321565284E-4	0.0	0.0	10.066985927079354	0.0
36	4.3205577321565284E-4	0.0	0.0	10.298654232677588	0.0
37	4.3205577321565284E-4	0.0	0.0	10.519348321636143	0.0
38	4.3205577321565284E-4	0.0	0.0	10.763114188884416	0.0
39	4.3205577321565284E-4	0.0	0.0	11.0431727410828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	35	1.211938E-7	45.000004	1
CGTAACT	35	1.211938E-7	45.000004	33
CGCGTAA	35	1.211938E-7	45.000004	31
GCGTAAC	35	1.211938E-7	45.000004	32
CGTTTTT	11350	0.0	43.770924	1
TAGCACG	40	3.4582445E-7	39.375	1
GCGATCG	80	0.0	39.375	9
GTTTTTT	12595	0.0	38.693928	2
ACGCTAG	35	6.248325E-6	38.57143	1
TTCGTAG	65	9.094947E-12	38.07692	1
CTATCGA	30	1.1399268E-4	37.499996	41
TATTACG	30	1.1399268E-4	37.499996	1
TAAGGGA	1260	0.0	37.142857	4
TAGGGAT	1370	0.0	37.116787	5
ATAACGG	85	0.0	37.058826	2
GACCGAT	765	0.0	37.058823	9
TCAGACG	195	0.0	36.923077	22
CATACGA	165	0.0	36.81818	18
CGACCCG	160	0.0	36.5625	32
GCGTAAG	105	0.0	36.42857	1
>>END_MODULE