FastQCFastQC Report
Thu 26 May 2016
SRR1547106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547106_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1659776
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169801.0230296136346109No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT207.0341944E-445.06
CGTTTTT131250.042.171431
TACGCGT356.2499676E-638.57142622
ACGCGTA356.2499676E-638.57142623
TAGTGCG1000.038.251
GCGTTAG1300.038.0769271
ACGGGTA1900.037.8947375
TAGGGAC13050.037.758625
AGGGCGA20500.037.7560966
TAATGCG900.037.5000041
TAGGGCG8700.037.55
CGCGTAT301.1401404E-437.49999624
GGGCGAT41150.037.3997577
ACGGGAT4300.037.151165
TACGGGA5400.037.0833324
TATAGCG552.750312E-936.818181
ATGGGCG7300.036.678085
ACCCGCT3700.036.48648534
CACGACC18950.036.33245527
AGGGAAC19950.036.2030076
Produced by FastQC (version 0.11.5)