##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547106_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1659776
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507926973278323	31.0	31.0	33.0	30.0	34.0
2	31.87345159828796	31.0	31.0	34.0	30.0	34.0
3	31.909848075884938	33.0	31.0	34.0	30.0	34.0
4	35.715516431132876	37.0	35.0	37.0	33.0	37.0
5	35.67691604168273	37.0	35.0	37.0	33.0	37.0
6	35.724427874604764	37.0	35.0	37.0	33.0	37.0
7	36.066759008444514	37.0	35.0	37.0	35.0	37.0
8	34.7631126127863	37.0	35.0	37.0	35.0	37.0
9	37.080695828834735	39.0	37.0	39.0	34.0	39.0
10	36.96411925464641	39.0	37.0	39.0	32.0	39.0
11	36.94003708934218	39.0	37.0	39.0	33.0	39.0
12	36.806454605922724	39.0	35.0	39.0	33.0	39.0
13	36.81341879868127	39.0	35.0	39.0	33.0	39.0
14	37.958482349425466	40.0	37.0	41.0	33.0	41.0
15	38.05525022653659	40.0	37.0	41.0	33.0	41.0
16	38.0511635305005	40.0	37.0	41.0	33.0	41.0
17	37.9236619881237	40.0	36.0	41.0	33.0	41.0
18	37.80860670355518	39.0	36.0	41.0	33.0	41.0
19	37.69806166615254	39.0	36.0	41.0	33.0	41.0
20	37.520363591231586	39.0	35.0	41.0	33.0	41.0
21	37.43400615504743	39.0	35.0	41.0	33.0	41.0
22	37.35124558976633	39.0	35.0	41.0	32.0	41.0
23	37.24552529979949	39.0	35.0	41.0	32.0	41.0
24	37.10020990784299	39.0	35.0	41.0	32.0	41.0
25	37.007354606886715	39.0	35.0	41.0	32.0	41.0
26	36.92346316611398	39.0	35.0	41.0	32.0	41.0
27	36.82789545095242	39.0	35.0	41.0	31.0	41.0
28	36.83170439866584	39.0	35.0	41.0	31.0	41.0
29	36.77048710187399	39.0	35.0	41.0	31.0	41.0
30	36.6717906512686	39.0	35.0	41.0	31.0	41.0
31	36.53590845993676	39.0	35.0	41.0	31.0	41.0
32	36.34519959319812	39.0	35.0	41.0	30.0	41.0
33	36.06884061463715	39.0	35.0	41.0	29.0	41.0
34	35.759501282100715	39.0	35.0	41.0	27.0	41.0
35	35.535519250790465	39.0	35.0	41.0	25.0	41.0
36	35.36610723374721	38.0	35.0	41.0	25.0	41.0
37	35.312337929937534	38.0	35.0	41.0	24.0	41.0
38	35.154013553636155	38.0	35.0	41.0	24.0	41.0
39	35.07668926409347	38.0	34.0	41.0	23.0	41.0
40	34.93118107503663	38.0	34.0	40.0	23.0	41.0
41	34.82402203670857	38.0	34.0	40.0	23.0	41.0
42	34.75330406030693	38.0	34.0	40.0	23.0	41.0
43	34.6749784308244	38.0	34.0	40.0	23.0	41.0
44	34.54252381044189	38.0	34.0	40.0	22.0	41.0
45	34.416136273810444	37.0	34.0	40.0	22.0	41.0
46	34.333987839322894	37.0	34.0	40.0	22.0	41.0
47	34.270292497300844	37.0	34.0	40.0	22.0	41.0
48	34.201430192989896	37.0	33.0	40.0	22.0	41.0
49	34.111294536130174	37.0	33.0	40.0	22.0	41.0
50	34.020788347343256	37.0	33.0	40.0	21.0	41.0
51	33.66841368955811	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	7.0
13	5.0
14	17.0
15	46.0
16	121.0
17	280.0
18	657.0
19	1338.0
20	2264.0
21	3717.0
22	5774.0
23	8751.0
24	13568.0
25	21624.0
26	31857.0
27	36432.0
28	36059.0
29	35719.0
30	38527.0
31	44526.0
32	53601.0
33	68333.0
34	105017.0
35	142635.0
36	133506.0
37	176449.0
38	260958.0
39	437691.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.76853262126937	22.862181402791702	25.548567999537287	17.820717976401635
2	35.92894462867278	23.956184448985887	26.8090995411429	13.305771381198428
3	28.692606713195033	24.662665323513536	33.55537132721524	13.089356636076193
4	24.940895638929593	27.25795529035243	32.94727722295057	14.853871847767412
5	22.474116989280482	30.391028668928822	32.55119967995682	14.583654661833886
6	20.65357012030539	38.979717745045114	30.129909096167196	10.2368030384823
7	84.69745315030463	3.0922847420374797	10.73343631911776	1.4768257885401403
8	82.4389556181075	5.4388664494486	10.212341906377729	1.9098360260661678
9	80.08086633377035	4.5858597786689295	12.397335544073416	2.935938343487314
10	34.914711382740805	30.343431884784454	20.197725476208838	14.544131256265905
11	29.252802787846072	26.67010488162258	27.85056537749672	16.226526953034625
12	25.516997474357982	24.0352915092157	31.27976305236369	19.16794796406262
13	22.920020532891186	25.750161467571527	33.03614463638467	18.293673363152617
14	21.261965470039332	26.77138360839053	33.22598953111745	18.740661390452686
15	21.335650111822318	23.861292126166422	35.95810519009794	18.844952571913318
16	23.929735096784142	22.856879482532584	34.63985501658055	18.573530404102723
17	22.80373978175368	23.09926158710573	32.32032515230971	21.77667347883088
18	24.6786915824786	22.62124527647104	32.492999055294206	20.207064085756148
19	26.045864020205133	24.946559053751834	29.64032495951261	19.367251966530425
20	28.137712558803113	24.333102780134187	28.989694994987275	18.539489666075422
21	26.44314654507596	24.678932578854017	30.350963021516158	18.526957854553867
22	25.138211421300223	21.975194243078583	30.773309169430092	22.1132851661911
23	23.305795480835968	26.597685470810518	30.008567421145987	20.087951627207527
24	21.960071720521324	25.084830724145906	33.56266146757152	19.39243608776124
25	23.284828796174907	25.119052209454768	31.79537479756305	19.80074419680728
26	21.97664022133107	26.260049548854784	30.48887319734711	21.27443703246703
27	22.138409038328064	24.79906927199815	31.152215720675564	21.910305968998227
28	20.905531830801266	26.79427826405491	31.96581948407496	20.33437042106887
29	21.833910117991824	24.956018161486853	31.696445785455385	21.513625935065935
30	22.841274967224493	24.357804908614174	32.68284395002699	20.11807617413434
31	24.331054310943163	25.622252641320276	30.04471687745816	20.0019761702784
32	23.687834984961825	24.94818577928588	30.487306720906915	20.876672514845378
33	23.02274523791162	25.81161554330223	28.96854756304465	22.197091655741495
34	21.50043138351199	27.80766802267294	30.364037074882393	20.327863518932677
35	22.238784028688208	27.99516320274543	29.221714255417595	20.544338513148762
36	24.297194320197423	28.954991516927585	26.67534655278785	20.072467610087145
37	23.73772123467263	28.298095646641475	26.992738779208757	20.971444339477134
38	23.38466154469037	28.448055641243158	27.959676486465646	20.207606327600832
39	23.84153042338243	27.037323108660445	26.430674982648263	22.690471485308862
40	24.867090498958895	26.379222256497265	28.049869379964527	20.703817864579317
41	21.818305602683736	26.851936646872833	28.132229891262433	23.197527859180997
42	23.69572761625665	27.157459801804578	27.090342311251636	22.05647027068713
43	23.034554060306935	26.05508213156474	28.323822009716977	22.586541798411353
44	23.42111224647181	27.001233901442124	28.008538501580936	21.56911535050513
45	22.54249971080435	26.48712838358911	27.7456114560037	23.224760449602837
46	23.041904449757077	26.74204229968381	27.854240572221794	22.361812678337316
47	22.529666653813525	27.15282062157785	29.156343988586414	21.16116873602221
48	23.236629521092002	26.61051852780134	28.76165217475129	21.391199776355364
49	23.058472950566824	26.616181942623584	28.65904796791856	21.66629713889103
50	22.074605247937072	27.30603406724763	29.124833712500966	21.494526972314336
51	22.263968149919027	26.891821547003936	28.343583712500966	22.50062659057608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1106.0
1	1661.0
2	2216.0
3	16426.5
4	30637.0
5	21914.5
6	13192.0
7	13014.5
8	12837.0
9	13334.0
10	13831.0
11	13984.5
12	14138.0
13	13836.5
14	13535.0
15	13095.5
16	12656.0
17	12232.0
18	11808.0
19	11209.0
20	10610.0
21	10493.5
22	10377.0
23	10422.0
24	10467.0
25	11152.0
26	13065.5
27	14294.0
28	16501.0
29	18708.0
30	21474.5
31	24241.0
32	27763.0
33	31285.0
34	37093.5
35	42902.0
36	47093.0
37	51284.0
38	55987.0
39	60690.0
40	67303.5
41	73917.0
42	80859.0
43	87801.0
44	94174.0
45	100547.0
46	106186.0
47	111825.0
48	116612.0
49	121399.0
50	123317.0
51	125235.0
52	120241.5
53	115248.0
54	108372.5
55	101497.0
56	95937.0
57	90377.0
58	84076.5
59	77776.0
60	70934.0
61	64092.0
62	57351.5
63	50611.0
64	44515.5
65	38420.0
66	33093.5
67	27767.0
68	23555.0
69	19343.0
70	16744.0
71	14145.0
72	12006.0
73	9867.0
74	8782.5
75	6605.5
76	5513.0
77	3976.5
78	2440.0
79	1659.5
80	879.0
81	675.0
82	471.0
83	314.0
84	157.0
85	106.5
86	56.0
87	38.0
88	20.0
89	15.5
90	11.0
91	8.5
92	6.0
93	6.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1659776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.33837781593708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19440341314535	21.902610869441812
2	6.66824400917522	4.046074122384336
3	3.2382845575221504	2.947329011448649
4	2.314506757756752	2.8087352189745562
5	1.7380262397915809	2.6364448358404715
6	1.4376199908523664	2.616903506293386
7	1.2391975994358464	2.6316671472200874
8	1.0972427613748301	2.663085236031335
9	0.9616395520067285	2.625712564637574
>10	8.97842413354321	49.93984334001636
>50	0.0994694568423936	1.9513464044715556
>100	0.03131479472505188	1.9003221792967848
>500	0.0012200503713902893	0.21129024290311138
>1k	2.0334172856504822E-4	0.07112924089555837
>5k	0.0	0.0
>10k+	2.0334172856504822E-4	1.0475060801444762
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16980	1.0230296136346109	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.024909385362844E-5	0.0	0.0	0.028256825017351737	0.0
2	6.024909385362844E-5	0.0	0.0	0.08718043880620036	0.0
3	6.024909385362844E-5	0.0	0.0	0.15417743117143518	0.0
4	6.024909385362844E-5	0.0	0.0	0.24599705020436494	0.0
5	6.024909385362844E-5	0.0	0.0	0.42204490244466725	0.0
6	6.024909385362844E-5	0.0	0.0	0.8639720058610318	0.0
7	6.024909385362844E-5	0.0	0.0	1.0921955733785764	0.0
8	6.024909385362844E-5	0.0	0.0	1.552619148608005	0.0
9	6.024909385362844E-5	0.0	0.0	1.8389830916943009	0.0
10	6.024909385362844E-5	0.0	0.0	2.2296984653350815	0.0
11	6.024909385362844E-5	0.0	0.0	2.482383164957199	0.0
12	6.024909385362844E-5	0.0	0.0	2.6846996221176833	0.0
13	6.024909385362844E-5	0.0	0.0	2.818091115909617	0.0
14	6.024909385362844E-5	0.0	0.0	2.8980416634533817	0.0
15	6.024909385362844E-5	0.0	0.0	2.9651591540063236	0.0
16	6.024909385362844E-5	0.0	0.0	3.0583042531040334	0.0
17	6.024909385362844E-5	0.0	0.0	3.1671141166036865	0.0
18	6.024909385362844E-5	0.0	0.0	3.310024967224493	0.0
19	6.024909385362844E-5	0.0	0.0	3.3908190020822087	0.0
20	6.024909385362844E-5	0.0	0.0	3.4775776972314336	0.0
21	6.024909385362844E-5	0.0	0.0	3.5745787383357754	0.0
22	6.024909385362844E-5	0.0	0.0	3.6818823744890876	0.0
23	6.024909385362844E-5	0.0	0.0	3.8103334425850237	0.0
24	6.024909385362844E-5	0.0	0.0	3.917576829644482	0.0
25	6.024909385362844E-5	0.0	0.0	4.009577195958973	0.0
26	6.024909385362844E-5	0.0	0.0	4.104529767872291	0.0
27	1.2049818770725688E-4	0.0	0.0	4.2094234402714585	0.0
28	1.2049818770725688E-4	0.0	0.0	4.312871134418138	0.0
29	1.2049818770725688E-4	0.0	0.0	4.433007827562274	0.0
30	1.2049818770725688E-4	0.0	0.0	4.581461594817614	0.0
31	1.2049818770725688E-4	0.0	0.0	4.699188324207604	0.0
32	1.2049818770725688E-4	0.0	0.0	4.837821489164803	0.0
33	1.8074728156088532E-4	0.0	0.0	4.964645831726691	0.0
34	1.8074728156088532E-4	0.0	0.0	5.095627361764479	0.0
35	1.8074728156088532E-4	0.0	0.0	5.238899706948407	0.0
36	1.8074728156088532E-4	0.0	0.0	5.370664475206293	0.0
37	1.8074728156088532E-4	0.0	0.0	5.507550416441736	0.0
38	1.8074728156088532E-4	0.0	0.0	5.67895908845531	0.0
39	1.8074728156088532E-4	0.0	0.0	5.922485925811676	6.024909385362844E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTAAT	20	7.0341944E-4	45.0	6
CGTTTTT	13125	0.0	42.17143	1
TACGCGT	35	6.2499676E-6	38.571426	22
ACGCGTA	35	6.2499676E-6	38.571426	23
TAGTGCG	100	0.0	38.25	1
GCGTTAG	130	0.0	38.076927	1
ACGGGTA	190	0.0	37.894737	5
TAGGGAC	1305	0.0	37.75862	5
AGGGCGA	2050	0.0	37.756096	6
TAATGCG	90	0.0	37.500004	1
TAGGGCG	870	0.0	37.5	5
CGCGTAT	30	1.1401404E-4	37.499996	24
GGGCGAT	4115	0.0	37.399757	7
ACGGGAT	430	0.0	37.15116	5
TACGGGA	540	0.0	37.083332	4
TATAGCG	55	2.750312E-9	36.81818	1
ATGGGCG	730	0.0	36.67808	5
ACCCGCT	370	0.0	36.486485	34
CACGACC	1895	0.0	36.332455	27
AGGGAAC	1995	0.0	36.203007	6
>>END_MODULE