FastQCFastQC Report
Thu 26 May 2016
SRR1547105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547105_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1192670
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140421.1773583640068082No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA33510.2809662354213655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC351.2119744E-745.013
ATACGAC253.8908944E-545.010
CGATCGA207.0333946E-445.010
CGTTTCG253.8908944E-545.042
TCGATTG302.1655705E-644.9999961
CGTTTTT91250.041.621921
TACGGGA3650.040.6849334
AATAGCG501.0822987E-940.51
CACGACC15650.038.9616627
ACAACGA5100.038.8235313
ACGACCA15650.038.8178928
CATTAGA6100.038.72950717
ACGGGAT2500.038.75
TGCATTA6000.038.62515
GTATGCG356.2484887E-638.5714261
GCGAGAC16150.038.4520121
AACACGT15150.038.31683341
AGACACG16150.038.31269524
GACACGA16050.038.27102725
ACACGAC16000.038.2526
Produced by FastQC (version 0.11.5)