FastQCFastQC Report
Thu 26 May 2016
SRR1547104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547104_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1248570
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112440.9005502294625051No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA14530.1163731308617058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG453.8562575E-1045.0000041
CGTTTTT68250.040.8461531
ACGGGTA1000.040.55
TCGATAG501.0822987E-940.51
TAGTACG403.4585355E-739.3751
CACGACC7650.038.8235327
TCGTTAA356.2487234E-638.5714267
AGGGCGA14100.038.297876
GCGAGAC7850.038.1210221
GGTACCT7150.038.0769278
GGCGATA6350.037.9133878
GTTGATC7200.037.81250416
CGTATGG1250.037.82
AACACGT7500.037.841
TAAGGGA10450.037.6794244
GACACGA7900.037.59493625
ACGTATG601.5643309E-1037.5000041
GGGCGAT28900.037.4480977
TAGTAAG2600.037.211541
CGAGACA8150.036.99386622
Produced by FastQC (version 0.11.5)