FastQCFastQC Report
Thu 26 May 2016
SRR1547103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547103_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences544327
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.49565794090684456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14490.266200280346189No Hit
GAGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT7130.13098743953542633No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTT6490.11922980120405566No Hit
AAGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT5750.10563503188340832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGT351.2103555E-745.00000410
CGACAGG351.2103555E-745.0000042
ACGGGAT453.8380676E-1045.0000045
TATTCCG351.2103555E-745.0000041
AACGTTG207.03001E-445.01
GCCCCAC207.03001E-445.026
CTAGACG207.03001E-445.01
TCGTTTA253.8880906E-545.016
TCGCAAG253.8880906E-545.01
AAATCCG207.03001E-445.01
CGAACAC207.03001E-445.036
CGTATTG253.8880906E-545.040
AATCACG253.8880906E-545.038
AGTCACG406.8030204E-945.01
GTCCTAT207.03001E-445.017
CAACCGT207.03001E-445.05
GTTAGTC253.8880906E-545.035
TTCGTGC207.03001E-445.014
TAACGAG207.03001E-445.01
AATATTG207.03001E-445.024