##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547103_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	544327
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494743049674184	31.0	31.0	33.0	30.0	34.0
2	31.84481387107382	31.0	31.0	34.0	30.0	34.0
3	31.930324970100692	33.0	31.0	34.0	30.0	34.0
4	35.65820361657607	37.0	35.0	37.0	33.0	37.0
5	35.58884089894494	37.0	35.0	37.0	33.0	37.0
6	35.63839934451166	37.0	35.0	37.0	33.0	37.0
7	36.00704723447487	37.0	35.0	37.0	35.0	37.0
8	34.688970049253484	37.0	35.0	37.0	33.0	37.0
9	37.026300367242484	39.0	37.0	39.0	34.0	39.0
10	37.00857389032696	39.0	37.0	39.0	32.0	39.0
11	36.800741098641076	39.0	35.0	39.0	32.0	39.0
12	35.84518680866464	37.0	35.0	39.0	32.0	39.0
13	35.34155939352632	37.0	35.0	39.0	30.0	39.0
14	36.30451180999656	38.0	35.0	41.0	31.0	41.0
15	36.73402017537252	38.0	35.0	41.0	32.0	41.0
16	36.92853376738615	38.0	35.0	41.0	32.0	41.0
17	36.85855193661163	38.0	35.0	41.0	32.0	41.0
18	36.839315338022914	38.0	35.0	40.0	32.0	41.0
19	36.73731231410531	37.0	35.0	40.0	32.0	41.0
20	36.54477914929812	37.0	35.0	40.0	32.0	41.0
21	36.38668116775394	37.0	35.0	40.0	32.0	41.0
22	36.288350568683896	36.0	35.0	40.0	31.0	41.0
23	36.27902161752035	36.0	35.0	40.0	31.0	41.0
24	36.12313370455627	36.0	35.0	40.0	31.0	41.0
25	36.01902716565594	35.0	35.0	40.0	31.0	41.0
26	35.907741118849515	35.0	35.0	40.0	31.0	41.0
27	35.80341963562344	35.0	35.0	40.0	31.0	41.0
28	35.85990773928172	36.0	35.0	40.0	31.0	41.0
29	35.85235161952282	36.0	35.0	40.0	31.0	41.0
30	35.818212214349096	36.0	35.0	40.0	31.0	41.0
31	35.6270513863909	36.0	35.0	40.0	30.0	41.0
32	35.4447822724208	35.0	34.0	40.0	30.0	41.0
33	35.24914986763471	35.0	34.0	40.0	29.0	41.0
34	35.14123863045559	36.0	34.0	40.0	29.0	41.0
35	35.01461805128167	35.0	34.0	40.0	29.0	41.0
36	34.79493576471496	35.0	34.0	40.0	27.0	41.0
37	34.671109461775735	35.0	34.0	40.0	26.0	41.0
38	34.616576065490044	35.0	34.0	40.0	27.0	41.0
39	34.57163065583739	35.0	34.0	40.0	26.0	41.0
40	34.34422323346077	35.0	34.0	40.0	24.0	41.0
41	34.30637098655771	35.0	34.0	40.0	24.0	41.0
42	34.24474442752243	35.0	34.0	40.0	24.0	41.0
43	34.125582600164975	35.0	33.0	40.0	24.0	41.0
44	34.02098922155248	35.0	34.0	40.0	23.0	41.0
45	33.87349148581643	35.0	34.0	40.0	23.0	41.0
46	33.82876102048952	35.0	34.0	40.0	23.0	41.0
47	33.78421059399956	35.0	33.0	39.0	23.0	41.0
48	33.75291139333525	35.0	34.0	39.0	23.0	41.0
49	33.73963812193773	35.0	34.0	39.0	24.0	41.0
50	33.576166532249914	35.0	33.0	39.0	23.0	41.0
51	33.19683205132209	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	0.0
14	11.0
15	21.0
16	47.0
17	129.0
18	266.0
19	572.0
20	956.0
21	1542.0
22	2313.0
23	3421.0
24	4967.0
25	6949.0
26	9152.0
27	10588.0
28	11264.0
29	12467.0
30	14123.0
31	17004.0
32	21239.0
33	28220.0
34	53695.0
35	103712.0
36	35396.0
37	42149.0
38	61255.0
39	102737.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15507038967385	24.597530528524214	28.63076790238221	13.616631179419725
2	29.820126504839923	27.213605057254185	29.53353407051276	13.43273436739313
3	28.03700716664799	26.279056523009146	31.777222147716355	13.90671416262651
4	24.604327913184537	29.33934932494622	30.57390134974014	15.482421412129105
5	22.453782377137273	32.49774492171066	30.340585714102	14.707886987050065
6	20.187497588765577	41.95933694268335	27.192845477075362	10.660319991475713
7	85.49603455275965	5.061663301655071	7.605538582506472	1.836763563078811
8	83.59864566703472	6.516671045162191	6.5008717186544125	3.383811569148692
9	81.91620110705513	5.421189836256515	9.608195073916965	3.054413982771386
10	56.65895684028167	18.74094064780913	12.88784131597367	11.712261195935532
11	50.20070656057848	19.36133978288786	18.6889498408126	11.749003815721066
12	43.88354794085173	20.528469100375325	21.917340128268485	13.670642830504459
13	19.387794469133443	44.63842506434551	23.534199111930878	12.439581354590164
14	13.385336387869792	45.4623783130361	27.720285784096692	13.43199951499742
15	12.00528358872516	21.905582489937114	52.670545462562025	13.418588458775698
16	13.449268546296619	16.72615909186941	50.716021803070575	19.10855055876339
17	13.952091298061642	17.806575826663018	28.372099859092053	39.86923301618329
18	20.026013774808156	22.485013603954975	35.028209146340345	22.460763474896524
19	28.615336002072283	24.896799166677383	25.244200636749603	21.243664194500735
20	30.67090186597395	22.98305981514788	24.653930449895007	21.692107868983165
21	20.76986811236628	29.549700823218394	28.543504180391565	21.136926884023758
22	23.029906655374436	24.518717609084245	24.36880772035927	28.08256801518205
23	18.465554712516557	31.99290132585744	23.139583375434253	26.40196058619176
24	19.481488149586554	23.85937129703285	40.04302560776886	16.616114945611738
25	15.96797513259493	25.247323759431374	37.109678557190804	21.675022550782895
26	15.60569290150957	35.20861540948731	27.594074885133384	21.59161680386973
27	17.73309058709195	34.13867032133258	27.9394555111174	20.188783580458068
28	14.271752090195783	29.239041972931712	39.26794004339303	17.22126589347947
29	14.980884652056575	24.74578699935884	36.98310023202964	23.29022811655494
30	17.920110521800314	31.686467876846088	30.6334243937927	19.759997207560897
31	29.471071616877353	26.897251100900743	23.51141868766385	20.12025859455805
32	30.471756866736357	26.8285424019018	25.327973809860616	17.371726921501228
33	28.19683756271506	27.222423286002716	23.95233012509025	20.628409026191978
34	19.517495916976376	28.482144005349724	27.207909951187432	24.792450126486468
35	20.42356892088763	25.894728720052466	30.478003112099895	23.203699246960007
36	33.23590415320203	23.249076382395142	24.993248543614406	18.521770920788423
37	21.228599720388665	31.105383344937877	28.19500043172578	19.471016502947677
38	21.918074980664194	32.58666206159165	22.671482399366557	22.823780558377592
39	21.450525143893284	31.13734942415129	25.91493716093451	21.49718827102091
40	25.0204380822557	25.408623860289865	24.20732390640185	25.363614151052584
41	17.73933683245549	23.95618810016773	26.908273886836405	31.396201180540373
42	23.42305268707964	25.8245503162621	23.597763844159854	27.15463315249841
43	23.00822850970097	25.867355468312248	26.42694556764592	24.697470454340863
44	21.02008535310576	30.562327424507696	27.12229964708714	21.295287575299408
45	17.806943252860872	36.23061137882192	22.153778886588395	23.808666481728814
46	23.988154179381144	30.904952354007794	25.591418393722893	19.515475072888172
47	22.785017094503857	25.754004486273875	26.823214722032894	24.637763697189374
48	24.621045805186956	21.92027953785133	30.740529130467532	22.718145526494187
49	22.758378695159344	20.65614970413005	32.57141387438065	24.014057726329945
50	19.79435155706037	31.4568265031865	27.025850270150116	21.722971669603012
51	18.831143779382614	32.28518886625135	23.68925296742583	25.194414386940206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	760.0
1	879.0
2	998.0
3	2785.5
4	4573.0
5	3367.5
6	2162.0
7	2427.0
8	2692.0
9	3084.5
10	3477.0
11	3593.0
12	3709.0
13	3685.5
14	3662.0
15	3502.0
16	3342.0
17	3327.5
18	3313.0
19	3103.0
20	2893.0
21	2760.5
22	2628.0
23	2614.5
24	2601.0
25	2487.0
26	2951.5
27	3530.0
28	3332.0
29	3134.0
30	4112.5
31	5091.0
32	5849.5
33	6608.0
34	7615.5
35	8623.0
36	9804.0
37	10985.0
38	13853.0
39	16721.0
40	26224.5
41	35728.0
42	44335.0
43	52942.0
44	54060.0
45	55178.0
46	54095.0
47	53012.0
48	52813.0
49	52614.0
50	48594.0
51	44574.0
52	40046.5
53	35519.0
54	31815.0
55	28111.0
56	24363.0
57	20615.0
58	19598.0
59	18581.0
60	16880.5
61	15180.0
62	13547.0
63	11914.0
64	10193.0
65	8472.0
66	7356.5
67	6241.0
68	5341.0
69	4441.0
70	3512.0
71	2583.0
72	2242.0
73	1901.0
74	1584.5
75	998.5
76	729.0
77	533.0
78	337.0
79	265.5
80	194.0
81	193.5
82	193.0
83	132.0
84	71.0
85	56.0
86	41.0
87	24.5
88	8.0
89	6.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.77193095341222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87568531386309	21.651982630326373
2	6.8303658937294305	3.6572416818693205
3	2.377200450996717	1.909267390095135
4	1.2411950962906355	1.329167576704269
5	0.7601601860530101	1.0175478007272085
6	0.4909367315192598	0.7885994567236497
7	0.36604344516957144	0.6859782888020228
8	0.334817453593787	0.7170967799688133
9	0.2899713272751123	0.6986783117050744
>10	4.643756767726555	32.37351033255516
>50	1.6721038424104797	29.823781724329628
>100	0.11294276365540308	4.032946552822325
>500	0.0034433769407135086	0.5496120359313956
>1k	0.0013773507762854034	0.7645894374396168
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2698	0.49565794090684456	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1449	0.266200280346189	No Hit
GAGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	713	0.13098743953542633	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTT	649	0.11922980120405566	No Hit
AAGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	575	0.10563503188340832	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.079364058736752	0.0
2	0.0	0.0	0.0	0.2781416317764873	0.0
3	0.0	0.0	0.0	0.41004763680655193	0.0
4	0.0	0.0	0.0	0.8493056563426029	0.0
5	0.0	0.0	0.0	1.3221831729824167	0.0
6	0.0	0.0	0.0	2.2155799730676597	0.0
7	0.0	0.0	0.0	2.559674607359179	0.0
8	0.0	0.0	0.0	3.6127180904125646	0.0
9	0.0	0.0	0.0	4.06024319940036	0.0
10	0.0	0.0	0.0	4.775805719723622	0.0
11	0.0	0.0	0.0	5.399511690583051	0.0
12	0.0	0.0	0.0	5.938342209737897	0.0
13	0.0	0.0	0.0	6.1837829099052595	0.0
14	0.0	0.0	0.0	6.277109164160514	0.0
15	0.0	0.0	0.0	6.3814582043514285	0.0
16	0.0	0.0	0.0	6.6046696195485435	0.0
17	0.0	0.0	0.0	6.83008559193279	0.0
18	0.0	0.0	0.0	7.200451199370966	0.0
19	0.0	0.0	0.0	7.334194335390308	0.0
20	0.0	0.0	0.0	7.479327683543164	0.0
21	0.0	0.0	0.0	7.681779518561453	0.0
22	0.0	0.0	0.0	7.875780551029069	0.0
23	0.0	0.0	0.0	8.095685130445485	0.0
24	0.0	0.0	0.0	8.258087509897543	0.0
25	0.0	0.0	0.0	8.375112753914467	0.0
26	0.0	0.0	0.0	8.508121037538098	0.0
27	0.0	0.0	0.0	8.61577691350971	0.0
28	0.0	0.0	0.0	8.733353296823417	0.0
29	0.0	0.0	0.0	8.856441073104953	0.0
30	0.0	0.0	0.0	9.038317041043344	0.0
31	0.0	0.0	0.0	9.192636044142583	0.0
32	0.0	0.0	0.0	9.315907533523047	0.0
33	0.0	0.0	0.0	9.452773792224159	0.0
34	0.0	0.0	0.0	9.589272624727416	0.0
35	0.0	0.0	0.0	9.789152476360718	0.0
36	0.0	0.0	0.0	9.928774431545744	0.0
37	0.0	0.0	0.0	10.07868432027072	0.0
38	0.0	0.0	0.0	10.21481572657612	0.0
39	0.0	0.0	0.0	10.358295656838628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAGT	35	1.2103555E-7	45.000004	10
CGACAGG	35	1.2103555E-7	45.000004	2
ACGGGAT	45	3.8380676E-10	45.000004	5
TATTCCG	35	1.2103555E-7	45.000004	1
AACGTTG	20	7.03001E-4	45.0	1
GCCCCAC	20	7.03001E-4	45.0	26
CTAGACG	20	7.03001E-4	45.0	1
TCGTTTA	25	3.8880906E-5	45.0	16
TCGCAAG	25	3.8880906E-5	45.0	1
AAATCCG	20	7.03001E-4	45.0	1
CGAACAC	20	7.03001E-4	45.0	36
CGTATTG	25	3.8880906E-5	45.0	40
AATCACG	25	3.8880906E-5	45.0	38
AGTCACG	40	6.8030204E-9	45.0	1
GTCCTAT	20	7.03001E-4	45.0	17
CAACCGT	20	7.03001E-4	45.0	5
GTTAGTC	25	3.8880906E-5	45.0	35
TTCGTGC	20	7.03001E-4	45.0	14
TAACGAG	20	7.03001E-4	45.0	1
AATATTG	20	7.03001E-4	45.0	24
>>END_MODULE