FastQCFastQC Report
Thu 26 May 2016
SRR1547102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547102_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences628376
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.5401224744420537No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14540.23139012311100363No Hit
GAGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCT7660.12190153665957962No Hit
AAGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCT6730.1071014806421633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.1043960940583345No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTT6350.10105414592536952No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG253.8887792E-545.0000041
CTAGACG302.1639244E-645.0000041
TTAGACG253.8887792E-545.0000041
TGGCGTA302.1639244E-645.00000419
CAGCACG302.1639244E-645.0000041
AATGCGG1200.045.0000042
TACGAAT302.1639244E-645.00000412
AGTTATG600.045.0000041
TATAGCG302.1639244E-645.0000041
GCATCGA253.8887792E-545.0000049
TAGTATG551.8189894E-1245.0000041
TAGTAAG453.8380676E-1045.0000041
CTTACGA253.8887792E-545.00000435
ATTTACG207.0308434E-445.01
AGGTACG207.0308434E-445.01
CGAACAG207.0308434E-445.035
CAACGTT207.0308434E-445.042
ACGTTAT207.0308434E-445.044
GCAACTA207.0308434E-445.026
GTTTACG351.2107557E-745.01