##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547102_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	628376
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.449966262237897	31.0	31.0	33.0	30.0	34.0
2	31.808655963945153	31.0	31.0	34.0	30.0	34.0
3	31.9067230448012	33.0	31.0	34.0	30.0	34.0
4	35.65321559066546	37.0	35.0	37.0	33.0	37.0
5	35.575746050135585	37.0	35.0	37.0	33.0	37.0
6	35.62060613390709	37.0	35.0	37.0	33.0	37.0
7	35.98206010414147	37.0	35.0	37.0	35.0	37.0
8	34.669209199587506	37.0	35.0	37.0	33.0	37.0
9	37.00437954345806	39.0	37.0	39.0	33.0	39.0
10	36.94637287229302	39.0	37.0	39.0	32.0	39.0
11	36.74154009701198	39.0	35.0	39.0	32.0	39.0
12	35.82778304709282	37.0	35.0	39.0	32.0	39.0
13	35.401180185112096	37.0	35.0	39.0	30.0	39.0
14	36.363447999287054	38.0	35.0	41.0	31.0	41.0
15	36.75343424955759	38.0	35.0	41.0	32.0	41.0
16	36.942111092721554	38.0	35.0	41.0	32.0	41.0
17	36.878337173921345	38.0	35.0	41.0	32.0	41.0
18	36.83228512864909	38.0	35.0	40.0	32.0	41.0
19	36.75509726660471	37.0	35.0	40.0	32.0	41.0
20	36.56196290119291	37.0	35.0	40.0	32.0	41.0
21	36.39434510547825	37.0	35.0	40.0	31.0	41.0
22	36.29710237182833	37.0	35.0	40.0	31.0	41.0
23	36.29254140832877	37.0	35.0	40.0	31.0	41.0
24	36.11980565775905	36.0	35.0	40.0	31.0	41.0
25	35.998402230511665	36.0	35.0	40.0	31.0	41.0
26	35.84347269787516	36.0	35.0	40.0	31.0	41.0
27	35.75719155410137	35.0	35.0	40.0	30.0	41.0
28	35.8207999668988	36.0	35.0	40.0	31.0	41.0
29	35.838482055329926	36.0	35.0	40.0	31.0	41.0
30	35.82795173590335	36.0	35.0	40.0	31.0	41.0
31	35.654106776834254	36.0	35.0	40.0	30.0	41.0
32	35.42546819101939	35.0	34.0	40.0	30.0	41.0
33	35.24175016232319	36.0	34.0	40.0	29.0	41.0
34	35.110459342813854	36.0	34.0	40.0	29.0	41.0
35	34.993096489999616	35.0	34.0	40.0	28.0	41.0
36	34.79359650909647	35.0	34.0	40.0	26.0	41.0
37	34.68078347995468	35.0	34.0	40.0	26.0	41.0
38	34.58381128496314	35.0	34.0	40.0	26.0	41.0
39	34.55658077329497	35.0	34.0	40.0	26.0	41.0
40	34.34453257285447	35.0	34.0	40.0	24.0	41.0
41	34.335937400537254	35.0	34.0	40.0	24.0	41.0
42	34.27706341426153	35.0	34.0	40.0	24.0	41.0
43	34.15985333621908	35.0	33.0	40.0	24.0	41.0
44	34.02187225482832	35.0	34.0	40.0	23.0	41.0
45	33.91934128610895	35.0	33.0	40.0	23.0	41.0
46	33.888773600519436	35.0	33.0	40.0	23.0	41.0
47	33.84301755636753	35.0	33.0	39.0	23.0	41.0
48	33.80100608552841	35.0	33.0	39.0	23.0	41.0
49	33.769528116923624	35.0	33.0	39.0	24.0	41.0
50	33.60647128470852	35.0	33.0	39.0	24.0	41.0
51	33.23562644022051	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	5.0
15	22.0
16	64.0
17	165.0
18	367.0
19	652.0
20	1145.0
21	1798.0
22	2683.0
23	4025.0
24	5668.0
25	7898.0
26	10436.0
27	12012.0
28	13105.0
29	14623.0
30	16879.0
31	20142.0
32	24730.0
33	32703.0
34	61925.0
35	113842.0
36	43210.0
37	50252.0
38	72431.0
39	117481.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.34687511935529	24.61806307051829	29.1277833653736	13.907278444752825
2	29.940195042458654	26.83297898073765	29.660107960838733	13.566718015964963
3	28.2060422422244	26.374177244197742	31.288273263141814	14.131507250436046
4	24.48597654907253	29.444313595681564	31.210135332985345	14.859574522260557
5	22.991489172088052	32.280513577857846	30.40599895603906	14.321998294015048
6	21.540447120832113	40.74344023323615	26.874196341044215	10.84191630488752
7	86.37408175996538	4.61634435433562	7.278285612435867	1.7312882732631418
8	84.17444332692529	6.429908207824614	6.1792620978522415	3.2163863673978637
9	82.92439558480909	4.922053038308274	9.255286643665576	2.898264733217055
10	57.03129973137102	19.375660432607226	12.620946694335874	10.97209314168587
11	49.416909620991255	19.48530815944594	18.77952054184119	12.318261677721619
12	42.40964008810012	20.351668427820286	22.42367627025857	14.815015213821026
13	20.630641526729217	42.02770315861841	23.93312284364775	13.408532471004623
14	15.199657529886565	43.24703680598877	27.236559002889987	14.316746661234674
15	13.377977516646084	22.132926782690618	49.97135473028887	14.517740970374426
16	14.60208537563497	17.082288311456832	48.7361070441901	19.579519268718094
17	15.897328987739826	17.964880899334158	28.58670604860784	37.55108406431818
18	20.94462551084064	21.970762728048175	34.76230791755255	22.322303843558633
19	28.373457929647216	24.54931442321158	26.036481342380995	21.04074630476021
20	31.747393280456286	22.14247520592766	24.458604402459674	21.651527111156376
21	22.548124053114694	28.52066278788496	27.260907482144447	21.670305676855893
22	23.25454823226858	24.525125084344403	24.660871834697698	27.55945484868932
23	19.869791335124194	30.448330299056614	23.072809909990198	26.609068455828993
24	21.06827759176035	23.08745082562033	37.73186754427286	18.11240403834647
25	17.751632780373534	24.12997950271812	35.693438323551504	22.424949393356844
26	16.637172648223356	32.71894534482539	27.49850408035953	23.145377926591724
27	17.976657287993174	33.479954676817705	27.408271480769475	21.135116554419646
28	15.080143099036244	28.868066253326035	37.55999592600608	18.491794721631635
29	14.920207009815778	23.841617120959427	37.46562567634665	23.77255019287815
30	18.59459941181713	29.345169134403605	30.770907864081376	21.289323589697887
31	29.04869059289343	26.807039097610346	23.04909799228487	21.095172317211354
32	30.404089271391648	26.331845901180184	24.355640571886898	18.908424255541266
33	28.606280316243776	27.41240913083886	22.47905712503342	21.50225342788394
34	19.503609303983602	28.813321960100325	26.459317351394706	25.22375138452137
35	20.219263625599957	26.470457178504592	28.898143786522716	24.41213540937273
36	31.684691967866375	25.043286185341262	24.229442244770645	19.04257960202172
37	22.494812023374543	31.348587469922467	26.78396374145416	19.37263676524883
38	21.66505404407552	33.88353469897004	22.359701834570384	22.09170942238405
39	21.64579805721415	31.37261766840236	26.071492227583487	20.910092046800006
40	24.808235833322723	25.676505786344485	23.880129094682164	25.63512928565063
41	18.178447299069347	23.32807109119381	26.794785287789473	31.698696321947367
42	23.303245190777496	24.70590856429908	23.722420970883675	28.26842527403975
43	23.072014208053776	24.910403961959084	25.51195462589278	26.505627204094363
44	20.32970705437509	29.408029587380803	27.331406673711285	22.930856684532827
45	18.02201229836913	35.176232064878356	23.035093638203875	23.766661998548642
46	24.14923548957949	30.192114275529303	25.427291939857668	20.231358295033548
47	22.70360421149121	25.63433358371421	26.25338969024915	25.408672514545433
48	24.423752657644467	22.320712439685796	30.530924160057037	22.724610742612704
49	22.756597960456794	21.51991801087247	31.79243001005767	23.93105401861306
50	20.427419252167493	31.109876888996396	26.671451487644337	21.79125237119177
51	18.912721045997937	31.967643576457405	23.96574662304098	25.153888754503672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	925.0
1	994.5
2	1064.0
3	3312.0
4	5560.0
5	3943.5
6	2327.0
7	2651.5
8	2976.0
9	3328.0
10	3680.0
11	3909.5
12	4139.0
13	3986.0
14	3833.0
15	3744.0
16	3655.0
17	3425.0
18	3195.0
19	3226.5
20	3258.0
21	3095.0
22	2932.0
23	2840.0
24	2748.0
25	2511.5
26	2349.0
27	2423.0
28	2911.0
29	3399.0
30	4741.0
31	6083.0
32	7108.5
33	8134.0
34	8914.5
35	9695.0
36	11215.0
37	12735.0
38	15318.5
39	17902.0
40	27064.0
41	36226.0
42	46569.0
43	56912.0
44	59719.5
45	62527.0
46	61353.0
47	60179.0
48	59493.0
49	58807.0
50	53977.0
51	49147.0
52	45263.5
53	41380.0
54	38479.0
55	35578.0
56	31189.0
57	26800.0
58	24671.5
59	22543.0
60	20713.5
61	18884.0
62	17249.0
63	15614.0
64	13891.0
65	12168.0
66	10821.5
67	9475.0
68	7897.5
69	6320.0
70	5562.5
71	4805.0
72	4090.5
73	3376.0
74	2756.0
75	1730.5
76	1325.0
77	987.5
78	650.0
79	500.5
80	351.0
81	244.0
82	137.0
83	112.0
84	87.0
85	46.0
86	5.0
87	4.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	628376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.104526282577904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70910810974974	20.261639257846337
2	6.478076258511961	3.2525807138471494
3	2.2886800340490194	1.7236868420158473
4	1.253389267612412	1.2586297524430747
5	0.783910382429608	0.9839849399444895
6	0.5374479997045996	0.8095426460461826
7	0.407217589523726	0.715610327524848
8	0.3035287844060544	0.6095957076512571
9	0.2640589720754582	0.596616786415696
>10	5.019482019723593	33.102765717840455
>50	1.815404557735582	30.677544930207596
>100	0.13518970110796888	4.692434014406085
>500	0.0032188024073325923	0.5318684464064207
>1k	0.001287520962933037	0.7834999174045358
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3394	0.5401224744420537	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1454	0.23139012311100363	No Hit
GAGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCT	766	0.12190153665957962	No Hit
AAGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTCT	673	0.1071014806421633	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	656	0.1043960940583345	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTT	635	0.10105414592536952	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07193145505238902	0.0
2	0.0	0.0	0.0	0.25064610997237324	0.0
3	0.0	0.0	0.0	0.38559715838924463	0.0
4	0.0	0.0	0.0	0.81448050211975	0.0
5	0.0	0.0	0.0	1.2778973098908935	0.0
6	0.0	0.0	0.0	2.162717863190192	0.0
7	0.0	0.0	0.0	2.5147363998625027	0.0
8	0.0	0.0	0.0	3.503793906832852	0.0
9	0.0	0.0	0.0	3.9080104905343296	0.0
10	1.591403872840465E-4	0.0	0.0	4.525952614358283	0.0
11	1.591403872840465E-4	0.0	0.0	5.04459113651699	0.0
12	1.591403872840465E-4	0.0	0.0	5.500528346085783	0.0
13	1.591403872840465E-4	0.0	0.0	5.701840936000102	0.0
14	1.591403872840465E-4	0.0	0.0	5.77647777763632	0.0
15	1.591403872840465E-4	0.0	0.0	5.860981323284149	0.0
16	1.591403872840465E-4	0.0	0.0	6.051313226475868	0.0
17	1.591403872840465E-4	0.0	0.0	6.237666619985486	0.0
18	1.591403872840465E-4	0.0	0.0	6.55515169261716	0.0
19	1.591403872840465E-4	0.0	0.0	6.665435981005003	0.0
20	1.591403872840465E-4	0.0	0.0	6.793384852381377	0.0
21	1.591403872840465E-4	0.0	0.0	6.956503749347524	0.0
22	1.591403872840465E-4	0.0	0.0	7.113416171209594	0.0
23	1.591403872840465E-4	0.0	0.0	7.315047041898481	0.0
24	6.36561549136186E-4	0.0	0.0	7.459864794326964	0.0
25	6.36561549136186E-4	0.0	0.0	7.577946961691726	0.0
26	6.36561549136186E-4	0.0	0.0	7.698416234865749	0.0
27	6.36561549136186E-4	0.0	0.0	7.807745680929889	0.0
28	6.36561549136186E-4	0.0	0.0	7.909754669178963	0.0
29	6.36561549136186E-4	0.0	0.0	8.025768011509033	0.0
30	6.36561549136186E-4	0.0	0.0	8.199231033648644	0.0
31	6.36561549136186E-4	0.0	0.0	8.343571364915274	0.0
32	6.36561549136186E-4	0.0	0.0	8.469769692031523	0.0
33	6.36561549136186E-4	0.0	0.0	8.59708200185876	0.0
34	6.36561549136186E-4	0.0	0.0	8.729486804079086	0.0
35	6.36561549136186E-4	0.0	0.0	8.915681057201422	0.0
36	7.957019364202325E-4	0.0	0.0	9.057156861496939	0.0
37	7.957019364202325E-4	0.0	0.0	9.19879180617974	0.0
38	7.957019364202325E-4	0.0	0.0	9.324035290972285	0.0
39	7.957019364202325E-4	0.0	0.0	9.454689548932487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.8887792E-5	45.000004	1
CTAGACG	30	2.1639244E-6	45.000004	1
TTAGACG	25	3.8887792E-5	45.000004	1
TGGCGTA	30	2.1639244E-6	45.000004	19
CAGCACG	30	2.1639244E-6	45.000004	1
AATGCGG	120	0.0	45.000004	2
TACGAAT	30	2.1639244E-6	45.000004	12
AGTTATG	60	0.0	45.000004	1
TATAGCG	30	2.1639244E-6	45.000004	1
GCATCGA	25	3.8887792E-5	45.000004	9
TAGTATG	55	1.8189894E-12	45.000004	1
TAGTAAG	45	3.8380676E-10	45.000004	1
CTTACGA	25	3.8887792E-5	45.000004	35
ATTTACG	20	7.0308434E-4	45.0	1
AGGTACG	20	7.0308434E-4	45.0	1
CGAACAG	20	7.0308434E-4	45.0	35
CAACGTT	20	7.0308434E-4	45.0	42
ACGTTAT	20	7.0308434E-4	45.0	44
GCAACTA	20	7.0308434E-4	45.0	26
GTTTACG	35	1.2107557E-7	45.0	1
>>END_MODULE