FastQCFastQC Report
Thu 26 May 2016
SRR1547101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547101_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1423943
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224761.5784339682136155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT158550.043.3538321
ACGGGTC2150.039.767445
ACGGGTA2100.039.6428575
CGTTCGG2100.038.5714345
TACGGGA2900.038.017244
TAAGGGT9000.038.04
ACGGGAT3100.037.741945
CGTTAGG1500.037.52
GTTTTTT182500.037.2698632
TAGGGCG5750.037.1739125
GGGCGAT29300.037.0136537
TAGGGAT10400.036.995195
TCGTAGG1850.036.4864852
ACGACCA8450.036.4792928
TAGGGTG11350.036.475775
ATTGGGC5000.036.454
GCGGGAT5200.036.3461535
ATAGGGC8550.036.315794
CGTAGGG6450.036.2790683
ACGGGAC3550.036.1267625
Produced by FastQC (version 0.11.5)