FastQCFastQC Report
Thu 26 May 2016
SRR1547100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547100_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1531071
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150890.9855192868260192No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG207.034023E-445.01
CGTTTTT106650.042.1097031
TGCGTAG850.039.7058831
TAGGGCG8950.039.4692735
TCGTTAG403.4592267E-739.3751
AGGGCGA18150.039.297526
AGTAGCG750.039.01
TACGGGA4350.038.7931024
ATAGGGC8000.037.968754
CGAATAT1600.037.9687514
TACGATC301.1400987E-437.50000411
ACAACGA6550.037.4427513
GGGCGAT32850.037.2602777
CGTAAGG2950.036.610172
AGGGAAC17650.036.5864036
CACAACG6800.036.3970612
ATAGGGT9050.036.298344
CACGACC16100.036.19565227
GCGATGA3550.036.1267629
CGTTAGG2500.036.0000042
Produced by FastQC (version 0.11.5)