FastQCFastQC Report
Thu 26 May 2016
SRR1547099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547099_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences423453
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105742.497089405435786No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT6470.15279145501389765No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC5320.12563377753847535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4560.10768609503297887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.2095006E-745.06
CGGGTAA207.028235E-445.06
TCGTTAG207.028235E-445.01
TCTAACG207.028235E-445.025
TCGTCGC351.2095006E-745.026
AATTACG207.028235E-445.01
TTCGAAT351.2095006E-745.016
AGTACGG406.7975634E-945.02
GTACGGG900.045.03
TAGTACG207.028235E-445.01
CTACGAA302.1622436E-644.99999611
TACGAAT302.1622436E-644.99999612
TACGAAG302.1622436E-644.9999961
CGTTTTT51650.043.9980621
ATACTAT2650.041.6037745
TACGGGT603.6379788E-1241.2499964
TAGGGCG2400.041.2499965
GCTTGTC2200.040.90909238
TGGGCGA2200.040.9090926
AGGGCGA5450.040.045876
Produced by FastQC (version 0.11.5)