##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547099_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	423453
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50921826035003	31.0	31.0	33.0	30.0	34.0
2	31.864074643466925	31.0	31.0	34.0	30.0	34.0
3	31.808788696738482	31.0	31.0	34.0	30.0	34.0
4	35.669429665157644	37.0	35.0	37.0	33.0	37.0
5	35.641188042120376	37.0	35.0	37.0	33.0	37.0
6	35.70227628567987	37.0	35.0	37.0	33.0	37.0
7	36.094565394506596	37.0	35.0	37.0	35.0	37.0
8	34.79542711942057	37.0	35.0	37.0	35.0	37.0
9	37.12097210316139	39.0	37.0	39.0	34.0	39.0
10	36.95692556198681	39.0	37.0	39.0	32.0	39.0
11	36.91297027060855	39.0	37.0	39.0	33.0	39.0
12	36.77861297475753	39.0	35.0	39.0	33.0	39.0
13	36.76375418287272	39.0	35.0	39.0	33.0	39.0
14	37.940163371141544	40.0	37.0	41.0	33.0	41.0
15	38.05941155216754	40.0	37.0	41.0	33.0	41.0
16	38.07502367441015	40.0	36.0	41.0	33.0	41.0
17	37.982517540317346	40.0	36.0	41.0	33.0	41.0
18	37.78932254583153	39.0	36.0	41.0	33.0	41.0
19	37.58819042491138	39.0	36.0	41.0	33.0	41.0
20	37.32004496366775	39.0	35.0	41.0	33.0	41.0
21	37.250509501644814	39.0	35.0	41.0	33.0	41.0
22	37.1710815604093	39.0	35.0	41.0	32.0	41.0
23	37.08408489253825	39.0	35.0	41.0	32.0	41.0
24	36.91164072518083	38.0	35.0	41.0	32.0	41.0
25	36.835034820865594	38.0	35.0	41.0	32.0	41.0
26	36.755734402637366	38.0	35.0	41.0	32.0	41.0
27	36.64295447192487	38.0	35.0	41.0	31.0	41.0
28	36.61582985596985	38.0	35.0	40.0	31.0	41.0
29	36.531582017366745	38.0	35.0	40.0	31.0	41.0
30	36.42119196227208	38.0	35.0	40.0	31.0	41.0
31	36.225174930866	38.0	35.0	40.0	31.0	41.0
32	35.90105867711411	38.0	35.0	41.0	30.0	41.0
33	35.51272750458729	38.0	35.0	41.0	27.0	41.0
34	35.10785848724652	38.0	35.0	41.0	24.0	41.0
35	34.80617211355215	38.0	34.0	41.0	22.0	41.0
36	34.61437042599769	38.0	34.0	41.0	21.0	41.0
37	34.530382356483486	38.0	34.0	40.0	20.0	41.0
38	34.43642860010438	38.0	34.0	40.0	20.0	41.0
39	34.33657808540735	38.0	34.0	40.0	18.0	41.0
40	34.21532023624818	38.0	34.0	40.0	18.0	41.0
41	34.13966130833882	38.0	34.0	40.0	18.0	41.0
42	34.0478258508028	38.0	34.0	40.0	18.0	41.0
43	33.96273730496655	37.0	33.0	40.0	18.0	41.0
44	33.832902352799486	37.0	33.0	40.0	17.0	41.0
45	33.72661428777219	37.0	33.0	40.0	18.0	41.0
46	33.65299100490491	37.0	33.0	40.0	17.0	41.0
47	33.58534004954505	37.0	33.0	40.0	17.0	41.0
48	33.49792066652025	37.0	33.0	40.0	17.0	41.0
49	33.417701610332195	36.0	33.0	40.0	15.0	41.0
50	33.32641402942003	36.0	33.0	40.0	15.0	41.0
51	33.00579521221954	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	4.0
15	17.0
16	36.0
17	96.0
18	187.0
19	412.0
20	753.0
21	1178.0
22	1846.0
23	2753.0
24	4333.0
25	7210.0
26	10276.0
27	11177.0
28	10122.0
29	9518.0
30	9937.0
31	11468.0
32	13463.0
33	17107.0
34	26720.0
35	42208.0
36	34921.0
37	41674.0
38	59150.0
39	106803.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.45285545267125	22.853303672426456	26.125685731356253	21.568155143546036
2	38.087816121269654	23.65008631418363	26.255097968369572	12.006999596177144
3	25.686203663688765	23.927094624432936	38.46117514812742	11.925526563750877
4	22.29527243873582	26.54415011819494	37.530729502447734	13.62984794062151
5	20.29788429884781	29.923037503571827	36.5913100155153	13.18776818206507
6	18.70549978391935	38.14779916543276	33.476914793377304	9.669786257270582
7	80.63374211541777	3.088890620682815	14.758662708730366	1.5187045551690508
8	78.72727315664312	5.444051642094872	13.962824681841903	1.8658505194201007
9	76.4297336422224	4.779042774522792	16.210299608220982	2.580923975033829
10	33.506434008024506	28.540357489497065	26.23738643958125	11.715822062897182
11	27.000162946064854	24.718681884412202	32.368881552380074	15.912273617142873
12	25.092985526138673	22.99145359697534	34.76395255199514	17.151608324890837
13	20.432728071356205	27.83638325859068	35.74564355430236	15.985245115750747
14	18.06953782356011	30.51625564112192	35.24877613336073	16.16543040195724
15	17.336044378006534	25.242470829112087	41.1332544579918	16.288230334889587
16	18.78555589404255	23.913397708836637	40.22028418738326	17.080762209737564
17	19.001872698977216	23.740533187862646	35.12857389131734	22.1290202218428
18	20.163985141208116	24.86745872623408	37.0685766779312	17.899979454626607
19	22.352893945727153	27.03747523337891	32.87472281457446	17.734908006319476
20	23.84373236226925	26.403402502757096	32.060700951463325	17.692164183510332
21	21.55138822962643	27.455703466500413	34.05242140213908	16.94048690173408
22	21.314998358731664	24.875724106335294	33.31420488224195	20.49507265269109
23	18.87529430657003	28.4914736700413	32.790888244976415	19.842343778412243
24	18.623082136624372	27.203255142837573	37.452562622061954	16.7211000984761
25	19.173084143930968	27.720667937173666	35.03340394329477	18.0728439756006
26	18.247833880029187	30.362991878673668	32.417293064401484	18.971881176895664
27	17.908953295879353	28.98645186124552	34.15349519309109	18.951099649784037
28	17.119727573071863	28.486278288263396	36.69002226929553	17.70397186936921
29	17.680828805085806	26.84123149440434	36.24345559011272	19.23448411039714
30	18.5985221500379	28.926704970799594	35.022068564870246	17.45270431429226
31	21.34404526594451	28.882544225687383	31.989146375158516	17.784264133209586
32	21.44771674778547	28.462190609111282	32.0356686574425	18.054423985660748
33	21.250292240225008	29.131686397309736	30.594894828942053	19.0231265335232
34	18.80208665424498	29.60352152423055	31.66018424713014	19.934207574394325
35	19.588006225011984	29.162858687977177	31.36404748578945	19.88508760122139
36	22.630374563410815	30.227675798730907	28.675437415722644	18.466512222135634
37	19.530148564303477	31.297452137545374	30.65794787142847	18.514451426722683
38	20.202950504542414	31.7955003270729	29.317775526445672	18.683773641939013
39	20.179571286541837	31.13474222641002	28.479429830465246	20.2062566565829
40	21.050270041775594	29.483791589621518	29.411292398447998	20.054645970154894
41	19.24251333678118	29.04525413682274	29.5640838534619	22.148148672934187
42	20.26623970074601	30.751228589713612	28.189196912053994	20.79333479748638
43	20.196338200461444	29.54023232802696	29.942874415814742	20.320555055696854
44	20.210979730926454	30.797750871997604	29.15364869300725	19.83762070406869
45	19.109558794010198	31.797625710527495	28.149759241285338	20.94305625417697
46	20.822145550982047	30.53042486415257	28.72172354428945	19.925706040575932
47	19.99230138882001	30.979116926789985	29.27574016478807	19.752841519601937
48	20.205075887997015	29.748992214011942	30.09472125595993	19.95121064203111
49	19.792515344087775	28.89364345039473	30.353545729986564	20.960295475530934
50	19.499212427353214	30.518144870859338	30.493584884272874	19.489057817514578
51	18.82334048879096	31.307134439949653	29.233940956847633	20.635584114411753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	553.0
1	616.0
2	679.0
3	7745.0
4	14811.0
5	9696.5
6	4582.0
7	4710.0
8	4838.0
9	5263.0
10	5688.0
11	6040.5
12	6393.0
13	6552.0
14	6711.0
15	6473.5
16	6236.0
17	5874.0
18	5512.0
19	5199.5
20	4887.0
21	4612.5
22	4338.0
23	4220.0
24	4102.0
25	4248.0
26	4744.5
27	5095.0
28	5383.0
29	5671.0
30	6346.5
31	7022.0
32	8075.0
33	9128.0
34	10638.0
35	12148.0
36	13331.5
37	14515.0
38	15829.0
39	17143.0
40	19547.0
41	21951.0
42	24022.0
43	26093.0
44	28001.5
45	29910.0
46	31444.5
47	32979.0
48	33052.5
49	33126.0
50	31179.5
51	29233.0
52	26785.0
53	24337.0
54	21606.5
55	18876.0
56	17322.5
57	15769.0
58	14264.0
59	12759.0
60	11500.5
61	10242.0
62	8976.5
63	7711.0
64	6719.0
65	5727.0
66	4802.5
67	3878.0
68	3104.5
69	2331.0
70	1989.5
71	1648.0
72	1303.0
73	958.0
74	802.0
75	555.0
76	464.0
77	327.5
78	191.0
79	141.5
80	92.0
81	68.0
82	44.0
83	33.0
84	22.0
85	16.5
86	11.0
87	8.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	423453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83835765189699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90459782447094	34.61569985022179
2	9.504222493922814	8.903243447471223
3	4.443777549048751	6.244177265034469
4	2.739116935783052	5.13182954754299
5	1.8880044205071242	4.421551314803761
6	1.4273931283821735	4.011404991421462
7	1.1487618320277635	3.7664282281755357
8	0.8791433151278523	3.2942103216986185
9	0.7509083086361665	3.1654180731313644
>10	3.2814501798201166	21.833459220284134
>50	0.019370507287224288	0.6451257857063489
>100	0.01172425441068704	1.1613219177185077
>500	0.0010195003835380034	0.2814964105654031
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.097501917690017E-4	2.524633626224404
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10574	2.497089405435786	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	647	0.15279145501389765	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	532	0.12563377753847535	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	456	0.10768609503297887	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07745841923424795	0.0
2	0.0	0.0	0.0	0.23355602628863179	0.0
3	0.0	0.0	0.0	0.3981551671614087	0.0
4	0.0	0.0	0.0	0.5618096931654752	0.0
5	0.0	0.0	0.0	0.9642156272360806	0.0
6	0.0	0.0	0.0	1.6450467938590587	0.0
7	0.0	0.0	0.0	2.0080150571610074	0.0
8	0.0	0.0	0.0	2.5259001589314516	0.0
9	0.0	0.0	0.0	2.847305368010145	0.0
10	0.0	0.0	0.0	3.260102065636564	0.0
11	0.0	0.0	0.0	3.674315685566049	0.0
12	0.0	0.0	0.0	3.9985547392508733	0.0
13	0.0	0.0	0.0	4.218413849943205	0.0
14	0.0	0.0	0.0	4.336490708531998	0.0
15	0.0	0.0	0.0	4.442759881261911	0.0
16	0.0	0.0	0.0	4.620347476579455	0.0
17	0.0	0.0	0.0	4.809270450321523	0.0
18	0.0	0.0	0.0	5.053453393883147	0.0
19	0.0	0.0	0.0	5.187588705240015	0.0
20	0.0	0.0	0.0	5.3325870875870525	0.0
21	0.0	0.0	0.0	5.492699307833455	0.0
22	0.0	0.0	0.0	5.667216904827691	0.0
23	0.0	0.0	0.0	5.864641412388152	0.0
24	0.0	0.0	0.0	6.018377482270759	0.0
25	0.0	0.0	0.0	6.1510958713245625	0.0
26	0.0	0.0	0.0	6.284522721529898	0.0
27	0.0	0.0	0.0	6.42999341131129	0.0
28	0.0	0.0	0.0	6.573338717638085	0.0
29	0.0	0.0	0.0	6.718809407419477	0.0
30	0.0	0.0	0.0	6.918831605868892	0.0
31	0.0	0.0	0.0	7.078707672398117	0.0
32	0.0	0.0	0.0	7.245196043008315	0.0
33	0.0	0.0	0.0	7.404599802103185	0.0
34	0.0	0.0	0.0	7.571560480147737	0.0
35	0.0	0.0	0.0	7.753871149808834	0.0
36	0.0	0.0	0.0	7.922248750156452	0.0
37	0.0	0.0	0.0	8.095113271130444	0.0
38	0.0	0.0	0.0	8.282147015135092	0.0
39	0.0	0.0	0.0	8.46705537568514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2095006E-7	45.0	6
CGGGTAA	20	7.028235E-4	45.0	6
TCGTTAG	20	7.028235E-4	45.0	1
TCTAACG	20	7.028235E-4	45.0	25
TCGTCGC	35	1.2095006E-7	45.0	26
AATTACG	20	7.028235E-4	45.0	1
TTCGAAT	35	1.2095006E-7	45.0	16
AGTACGG	40	6.7975634E-9	45.0	2
GTACGGG	90	0.0	45.0	3
TAGTACG	20	7.028235E-4	45.0	1
CTACGAA	30	2.1622436E-6	44.999996	11
TACGAAT	30	2.1622436E-6	44.999996	12
TACGAAG	30	2.1622436E-6	44.999996	1
CGTTTTT	5165	0.0	43.998062	1
ATACTAT	265	0.0	41.60377	45
TACGGGT	60	3.6379788E-12	41.249996	4
TAGGGCG	240	0.0	41.249996	5
GCTTGTC	220	0.0	40.909092	38
TGGGCGA	220	0.0	40.909092	6
AGGGCGA	545	0.0	40.04587	6
>>END_MODULE