FastQCFastQC Report
Thu 26 May 2016
SRR1547098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547098_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1047898
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.459109569824544No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13610.12987905311394812No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.033E-445.00000424
TTATACG351.2117926E-745.0000041
AACGCAT351.2117926E-745.0000045
TAATGCG351.2117926E-745.0000041
GTCGGTA207.033E-445.00000420
TTAGCGT253.8905695E-545.043
CTATTAG253.8905695E-545.01
TACGATA302.1653177E-644.99999620
TGCTACG302.1653177E-644.9999961
CGTTAGG1150.043.043482
ACGGGTA950.042.631585
CGAATAT800.042.18750414
CGCGTAA700.041.78571731
ACTAACC1300.041.5384614
CATACGA2450.041.3265318
CGGGTAT603.6379788E-1241.2499966
TATGGGC4300.040.8139574
CGTTTTT30800.040.2516251
TAGGGAC7700.039.740265
ACATACG2550.039.70588317
Produced by FastQC (version 0.11.5)