FastQCFastQC Report
Thu 26 May 2016
SRR1547097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547097_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences158641
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28631.8047037020694525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.014889E-445.039
ATGGGTA207.014889E-445.05
TTAATAG207.014889E-445.01
CACGGGC207.014889E-445.04
TCCGAAC207.014889E-445.035
TCTTCGA207.014889E-445.027
TAAACGG253.875563E-545.02
TACGGGT253.875563E-545.04
TAACGAG253.875563E-545.01
ACGGGAT253.875563E-545.05
GAACGTT207.014889E-445.038
GTCCGAA207.014889E-445.034
CGTAAGG253.875563E-545.02
TTACGGG406.7520887E-945.03
CTTTAGG253.875563E-545.02
GTACGGG253.875563E-545.03
GTACGAG207.014889E-445.01
CTATAGG302.1536434E-644.9999962
GGCAACG302.1536434E-644.9999968
TAGGGTA600.044.9999965