##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547097_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	158641
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19348718174999	33.0	31.0	34.0	31.0	34.0
2	32.61992801356522	34.0	31.0	34.0	31.0	34.0
3	32.63121135141609	34.0	31.0	34.0	31.0	34.0
4	36.30407649976992	37.0	37.0	37.0	35.0	37.0
5	36.30847637117769	37.0	37.0	37.0	35.0	37.0
6	36.36369538769927	37.0	37.0	37.0	35.0	37.0
7	36.45253749030831	37.0	37.0	37.0	35.0	37.0
8	35.420906323081674	37.0	37.0	37.0	35.0	37.0
9	37.69628910559061	39.0	39.0	39.0	35.0	39.0
10	37.8415226832912	39.0	38.0	39.0	35.0	39.0
11	37.8129172156	39.0	38.0	39.0	35.0	39.0
12	37.685333551856075	39.0	37.0	39.0	35.0	39.0
13	37.692866282991154	39.0	37.0	39.0	35.0	39.0
14	39.02462793351025	41.0	39.0	41.0	35.0	41.0
15	39.07598918312416	41.0	38.0	41.0	35.0	41.0
16	39.08490238967228	41.0	38.0	41.0	35.0	41.0
17	38.99638807117958	40.0	38.0	41.0	35.0	41.0
18	38.799931921760454	40.0	38.0	41.0	35.0	41.0
19	38.64101335720274	40.0	37.0	41.0	35.0	41.0
20	38.445111919365104	40.0	36.0	41.0	35.0	41.0
21	38.37455008478262	40.0	35.0	41.0	35.0	41.0
22	38.30771994629384	40.0	35.0	41.0	35.0	41.0
23	38.19416796414546	40.0	35.0	41.0	35.0	41.0
24	38.06963521409976	40.0	35.0	41.0	35.0	41.0
25	37.98974413928303	40.0	35.0	41.0	34.0	41.0
26	37.92257360959651	40.0	35.0	41.0	34.0	41.0
27	37.8224922939215	40.0	35.0	41.0	34.0	41.0
28	37.805895071261524	40.0	35.0	41.0	34.0	41.0
29	37.742141060633756	40.0	35.0	41.0	34.0	41.0
30	37.631394154096355	40.0	35.0	41.0	34.0	41.0
31	37.478501774446705	40.0	35.0	41.0	33.0	41.0
32	37.24859903807969	40.0	35.0	41.0	33.0	41.0
33	36.88639128598534	40.0	35.0	41.0	32.0	41.0
34	36.4939265385367	40.0	35.0	41.0	31.0	41.0
35	36.22405305059852	40.0	35.0	41.0	29.0	41.0
36	36.07927963136894	40.0	35.0	41.0	29.0	41.0
37	36.02744561620262	40.0	35.0	41.0	29.0	41.0
38	35.89569531205678	39.0	35.0	41.0	27.0	41.0
39	35.78999754161913	39.0	35.0	41.0	27.0	41.0
40	35.63820197805107	39.0	35.0	41.0	25.0	41.0
41	35.492338046280594	39.0	35.0	41.0	24.0	41.0
42	35.423805951803125	39.0	35.0	41.0	24.0	41.0
43	35.321657074778905	39.0	35.0	41.0	23.0	41.0
44	35.1812457057129	38.0	35.0	41.0	23.0	41.0
45	35.055937620161245	38.0	35.0	41.0	23.0	41.0
46	34.965406168644925	38.0	35.0	41.0	23.0	41.0
47	34.90044187820299	38.0	35.0	41.0	23.0	41.0
48	34.81978807496171	37.0	35.0	40.0	23.0	41.0
49	34.73387081523692	37.0	35.0	40.0	23.0	41.0
50	34.64222994055761	37.0	35.0	40.0	23.0	41.0
51	34.31823425217945	36.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	1.0
14	0.0
15	2.0
16	6.0
17	13.0
18	22.0
19	48.0
20	85.0
21	156.0
22	268.0
23	459.0
24	821.0
25	1552.0
26	2683.0
27	3387.0
28	3201.0
29	2650.0
30	2348.0
31	2477.0
32	2855.0
33	3952.0
34	8069.0
35	12621.0
36	11368.0
37	16165.0
38	27314.0
39	56069.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.88998430418366	22.007551641757175	25.276567848160315	19.825896205898854
2	39.10842720356024	22.8118834349254	25.01307984695003	13.06660951456433
3	28.187542942871012	24.40667923172446	34.51314603412737	12.892631791277159
4	24.889530449253343	26.70179840016137	33.864511696219765	14.544159454365518
5	22.15883661852863	29.48229020240669	33.63001998222401	14.728853196840666
6	20.676874200238274	37.922100844044095	31.460971627763314	9.940053327954313
7	82.34252179449196	3.458752781437333	12.752062833693687	1.4466625903770147
8	80.2686569045833	5.317036579446675	12.591322545874018	1.822983970096003
9	77.30221065172307	4.981688214269956	14.638082210777794	3.0780189232291777
10	37.038344438070865	28.490112896413915	21.44212404107387	13.02941862444135
11	29.06688687035508	26.5744668780454	28.294703134750787	16.063943116848733
12	25.52807912204285	22.704723243045617	33.53924899616114	18.227948638750384
13	23.690597008339584	23.705095151946846	34.273611487572566	18.330696352141
14	21.048783101468093	24.472866409061968	35.66795469014946	18.810395799320478
15	21.535416443416267	22.487251088936656	37.09318524215052	18.884147225496562
16	24.65377802711783	21.7573010760144	35.039491682478044	18.549429214389722
17	23.548136988546467	22.52507233312952	33.58085236477329	20.34593831355072
18	24.554182084076626	22.17711688655518	34.018948443340626	19.249752586027572
19	25.990759009335545	24.29321549914587	30.687527184019263	19.028498307499323
20	27.482176738674113	23.857010482788183	30.61818823633235	18.042624542205356
21	26.515843949546458	23.553810175175396	31.689790155130137	18.24055572014801
22	25.34212467142794	21.92119313418347	31.77047547607491	20.966206718313675
23	23.846924817670086	24.195510618314305	32.122213047068534	19.83535151694707
24	23.109410555909253	23.500860433305387	34.14501925731683	19.244709753468523
25	23.84440340139056	24.283760188097656	31.95453886448018	19.917297546031605
26	21.69300496088653	24.823973625985715	31.743370251070026	21.739651162057726
27	21.66211761146236	23.38361457630751	32.86729155766794	22.086976254562188
28	20.917669454932835	25.930875372696843	32.426674062821085	20.724781109549234
29	22.366223107519495	24.116716359579176	32.404611670375246	21.112448862526083
30	23.14218896754307	24.05178989038143	33.451629780447675	19.354391361627826
31	24.487364552669234	25.267742891181978	30.926431376504183	19.318461179644604
32	24.380204360789456	24.28754231251694	30.73354303112058	20.59871029557302
33	23.65403647228648	25.104481187082783	29.617185973361238	21.624296367269498
34	21.98422854117158	26.90540276473295	31.082759185834686	20.02760950826079
35	22.400262227293073	27.361148757256952	30.401346436293263	19.837242579156715
36	24.2623281497217	29.046085186049	27.04281995196702	19.64876671226228
37	23.306081025712142	28.615553356320245	27.61392073927925	20.464444878688358
38	23.63953832867922	28.074079210292417	28.428968551635453	19.857413909392907
39	23.234220661745702	27.530714002054957	26.79509080250377	22.43997453369558
40	24.619108553274373	26.070813976210438	28.687413720286685	20.622663750228504
41	21.390435007343626	27.060469865923693	28.905516228465526	22.643578898267155
42	22.707875013395025	27.278572374102534	27.657415170101046	22.356137442401398
43	22.271669997037336	25.74429056801205	29.441947541934304	22.54209189301631
44	22.59756305116584	26.454069250698115	29.095252803499726	21.853114894636317
45	22.156945556318984	26.50323686814884	28.491373604553676	22.8484439709785
46	22.35046425577247	26.826608505997818	28.7800757685592	22.042851469670513
47	21.77747240625059	27.570426308457463	29.8813043286414	20.77079695665055
48	22.338487528444727	27.044711014176663	29.563605877421345	21.05319557995726
49	23.0413323163621	26.738358936214475	29.285619732603802	20.934689014819625
50	21.90417357429668	26.972850650210223	30.147944100201084	20.975031675292012
51	21.672833630650338	26.754117787961494	29.848525917007585	21.724522664380583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	261.0
2	329.0
3	2624.5
4	4920.0
5	3321.5
6	1723.0
7	1663.0
8	1603.0
9	1623.0
10	1643.0
11	1624.0
12	1605.0
13	1562.0
14	1519.0
15	1462.5
16	1406.0
17	1363.0
18	1320.0
19	1245.0
20	1170.0
21	1124.0
22	1078.0
23	1097.5
24	1117.0
25	1199.0
26	1429.5
27	1578.0
28	1735.5
29	1893.0
30	2170.0
31	2447.0
32	2812.5
33	3178.0
34	3669.0
35	4160.0
36	4445.0
37	4730.0
38	5101.0
39	5472.0
40	5797.5
41	6123.0
42	6600.0
43	7077.0
44	7503.0
45	7929.0
46	8337.0
47	8745.0
48	9112.0
49	9479.0
50	9779.5
51	10080.0
52	9744.0
53	9408.0
54	9067.0
55	8726.0
56	8545.0
57	8364.0
58	8274.0
59	8184.0
60	7738.5
61	7293.0
62	6774.0
63	6255.0
64	5496.0
65	4737.0
66	4190.5
67	3644.0
68	3154.5
69	2665.0
70	2280.5
71	1896.0
72	1643.5
73	1391.0
74	1221.0
75	846.0
76	641.0
77	483.0
78	325.0
79	233.5
80	142.0
81	106.5
82	71.0
83	52.0
84	33.0
85	22.0
86	11.0
87	6.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	158641.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.07307956873669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36174189121161	49.20597951170359
2	16.609809513199615	22.28142150200794
3	6.0384512413344575	12.150525617458888
4	2.281108135260532	6.120037898448888
5	0.9029465126514711	3.028170164469271
6	0.34597565204462555	1.3923391463060835
7	0.15198912699239064	0.7136065165840408
8	0.07999426764666276	0.42923695031259435
9	0.061995556639676486	0.3742409613061028
>10	0.1549888913733541	1.8953493818988947
>50	0.007999426651351327	0.3225983908995792
>100	0.001999856662837832	0.16632931589001174
>500	0.0	0.0
>1k	9.99928331418916E-4	1.9201646427141272
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2863	1.8047037020694525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011976727327739991	0.0
2	0.0	0.0	0.0	0.05736222035917575	0.0
3	0.0	0.0	0.0	0.0857281535038231	0.0
4	0.0	0.0	0.0	0.1292225843256157	0.0
5	0.0	0.0	0.0	0.26222729307051773	0.0
6	0.0	0.0	0.0	0.5465169785868722	0.0
7	0.0	0.0	0.0	0.67700027105225	0.0
8	0.0	0.0	0.0	0.9373365019131246	0.0
9	0.0	0.0	0.0	1.1371587420654181	0.0
10	0.0	0.0	0.0	1.3811057671093854	0.0
11	0.0	0.0	0.0	1.5998386293581104	0.0
12	0.0	0.0	0.0	1.7448200654307524	0.0
13	0.0	0.0	0.0	1.8532409654502935	0.0
14	0.0	0.0	0.0	1.9288834538360198	0.0
15	0.0	0.0	0.0	1.9812028416361471	0.0
16	0.0	0.0	0.0	2.039195416065204	0.0
17	0.0	0.0	0.0	2.098448698634023	0.0
18	0.0	0.0	0.0	2.1892196846968943	0.0
19	0.0	0.0	0.0	2.2364962399379733	0.0
20	0.0	0.0	0.0	2.297010230646554	0.0
21	0.0	0.0	0.0	2.3537420969358487	0.0
22	0.0	0.0	0.0	2.4224506905528838	0.0
23	0.0	0.0	0.0	2.4955717626590856	0.0
24	0.0	0.0	0.0	2.571214251044812	0.0
25	0.0	0.0	0.0	2.6430746150112516	0.0
26	0.0	0.0	0.0	2.6909815243222117	0.0
27	0.0	0.0	0.0	2.763472242358533	0.0
28	0.0	0.0	0.0	2.837854022604497	0.0
29	0.0	0.0	0.0	2.9210607598287956	0.0
30	0.0	0.0	0.0	3.008049621472381	0.0
31	0.0	0.0	0.0	3.0780189232291777	0.0
32	0.0	0.0	0.0	3.1706809715016924	0.0
33	0.0	0.0	0.0	3.261451957564564	0.0
34	0.0	0.0	0.0	3.3528532976973167	0.0
35	0.0	0.0	0.0	3.455601011087928	0.0
36	0.0	0.0	0.0	3.5482630593604427	0.0
37	0.0	0.0	0.0	3.6459679401920058	0.0
38	0.0	0.0	0.0	3.7714084000983354	0.0
39	0.0	0.0	0.0	3.938452228616814	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.014889E-4	45.0	39
ATGGGTA	20	7.014889E-4	45.0	5
TTAATAG	20	7.014889E-4	45.0	1
CACGGGC	20	7.014889E-4	45.0	4
TCCGAAC	20	7.014889E-4	45.0	35
TCTTCGA	20	7.014889E-4	45.0	27
TAAACGG	25	3.875563E-5	45.0	2
TACGGGT	25	3.875563E-5	45.0	4
TAACGAG	25	3.875563E-5	45.0	1
ACGGGAT	25	3.875563E-5	45.0	5
GAACGTT	20	7.014889E-4	45.0	38
GTCCGAA	20	7.014889E-4	45.0	34
CGTAAGG	25	3.875563E-5	45.0	2
TTACGGG	40	6.7520887E-9	45.0	3
CTTTAGG	25	3.875563E-5	45.0	2
GTACGGG	25	3.875563E-5	45.0	3
GTACGAG	20	7.014889E-4	45.0	1
CTATAGG	30	2.1536434E-6	44.999996	2
GGCAACG	30	2.1536434E-6	44.999996	8
TAGGGTA	60	0.0	44.999996	5
>>END_MODULE