FastQCFastQC Report
Thu 26 May 2016
SRR1547096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547096_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences949248
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107281.1301577669902914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG302.1651013E-645.00000421
CGTTTTT69750.042.3225821
ACGTTAG650.041.538461
GCGATCG1100.040.9090929
CGTTAGG1650.039.5454562
CACAACG2300.039.13043612
GGGCGAT22300.038.6434947
CTATGCG356.247137E-638.571431
CGAGGGT3500.038.5714264
GCCGATT659.094947E-1238.076929
GCGATAT1300.038.076929
ATTGGGC3150.037.8571434
ACAACGA2450.037.6530613
TCTAGCG301.139772E-437.5000041
CTACGAA301.139772E-437.50000411
TATAGCG301.139772E-437.5000041
AGGGTAC6550.037.442756
AAGGGTA7950.037.358495
AGGGCGA12400.037.1975826
GGCGATA4800.037.0312548
Produced by FastQC (version 0.11.5)