##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547096_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	949248
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47819431802859	31.0	31.0	33.0	30.0	34.0
2	31.85863230683657	31.0	31.0	34.0	30.0	34.0
3	31.928852101874327	33.0	31.0	34.0	30.0	34.0
4	35.7304213440534	37.0	35.0	37.0	33.0	37.0
5	35.69003779834142	37.0	35.0	37.0	33.0	37.0
6	35.74108346817692	37.0	35.0	37.0	33.0	37.0
7	36.051166818365694	37.0	35.0	37.0	35.0	37.0
8	34.73807371730043	37.0	35.0	37.0	33.0	37.0
9	37.05662060915588	39.0	37.0	39.0	34.0	39.0
10	36.84030727481122	39.0	37.0	39.0	32.0	39.0
11	36.90462766316073	39.0	37.0	39.0	33.0	39.0
12	36.791068298274006	39.0	35.0	39.0	33.0	39.0
13	36.81126849885383	39.0	35.0	39.0	33.0	39.0
14	37.955763931027505	40.0	37.0	41.0	33.0	41.0
15	38.06086186117853	40.0	37.0	41.0	33.0	41.0
16	38.05408070388349	40.0	37.0	41.0	33.0	41.0
17	37.9424670897384	40.0	36.0	41.0	33.0	41.0
18	37.832240889630526	39.0	36.0	41.0	33.0	41.0
19	37.70567333299623	39.0	36.0	41.0	33.0	41.0
20	37.53565875303398	39.0	35.0	41.0	33.0	41.0
21	37.44986768473571	39.0	35.0	41.0	33.0	41.0
22	37.3755277862055	39.0	35.0	41.0	32.0	41.0
23	37.28911728020496	39.0	35.0	41.0	32.0	41.0
24	37.15919759641316	39.0	35.0	41.0	32.0	41.0
25	37.05612021305286	39.0	35.0	41.0	32.0	41.0
26	36.973969921453616	39.0	35.0	41.0	32.0	41.0
27	36.87617566747573	39.0	35.0	41.0	31.0	41.0
28	36.87838794498382	39.0	35.0	41.0	31.0	41.0
29	36.828292501011326	39.0	35.0	41.0	31.0	41.0
30	36.737810350930424	39.0	35.0	41.0	31.0	41.0
31	36.61576426813646	39.0	35.0	41.0	31.0	41.0
32	36.43315866875674	39.0	35.0	41.0	30.0	41.0
33	36.17515022417746	39.0	35.0	41.0	30.0	41.0
34	35.88499844087109	39.0	35.0	41.0	27.0	41.0
35	35.677640616572276	39.0	35.0	41.0	26.0	41.0
36	35.5430424925836	39.0	35.0	41.0	25.0	41.0
37	35.50176455467907	39.0	35.0	41.0	25.0	41.0
38	35.37655280811758	38.0	35.0	41.0	25.0	41.0
39	35.28935009607605	38.0	35.0	41.0	24.0	41.0
40	35.1673724885383	38.0	35.0	41.0	24.0	41.0
41	35.05733591221683	38.0	34.0	41.0	23.0	41.0
42	34.984284401968715	38.0	34.0	41.0	23.0	41.0
43	34.903467797667204	38.0	34.0	40.0	23.0	41.0
44	34.78198110504315	38.0	34.0	40.0	23.0	41.0
45	34.6704917998247	38.0	34.0	40.0	23.0	41.0
46	34.60293200512405	38.0	34.0	40.0	23.0	41.0
47	34.54715627528317	38.0	34.0	40.0	23.0	41.0
48	34.46190458131068	37.0	34.0	40.0	23.0	41.0
49	34.380279968985974	37.0	34.0	40.0	23.0	41.0
50	34.31241466929612	37.0	34.0	40.0	23.0	41.0
51	33.96730675229234	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	8.0
14	12.0
15	17.0
16	71.0
17	154.0
18	343.0
19	725.0
20	1353.0
21	2150.0
22	3238.0
23	4927.0
24	7444.0
25	11918.0
26	17060.0
27	19534.0
28	19858.0
29	19783.0
30	21398.0
31	25080.0
32	30154.0
33	38374.0
34	59729.0
35	83638.0
36	74206.0
37	97071.0
38	145435.0
39	265392.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.23314876618123	23.521671891855448	25.146853087918014	18.098326254045308
2	35.31132011866235	24.327783677184467	27.407800701186623	12.953095502966558
3	28.286601604638616	24.813325916936353	33.76693972491909	13.133132753505933
4	24.222015742988134	27.740906485976268	33.35798442556634	14.679093345469255
5	22.121194882686083	31.20649187567422	32.620242549892126	14.052070691747574
6	20.81352818230852	39.56500303398058	29.71457406283711	9.906894720873787
7	85.33786744875944	2.989840378910464	10.181322478425027	1.4909696939050703
8	82.94755427454153	5.333379685814455	9.844634911003237	1.8744311286407769
9	79.79621763754045	4.148336367313916	13.104583838996763	2.9508621561488675
10	33.99585777373247	23.4497201995685	29.43624848300971	13.118173543689322
11	30.699142900485437	25.207216659924487	27.219335726806904	16.87430471278317
12	26.416384337918014	23.495967334142396	31.113892259978428	18.973756067961165
13	23.202261158306364	25.394522822276162	32.61455383629989	18.788662183117584
14	21.23997100862999	26.591786340345198	33.61587277508091	18.552369875943906
15	21.506708468176917	23.557068331984897	36.51532581580367	18.42089738403452
16	23.660202602481125	22.60768524137001	35.0975719727616	18.634540183387273
17	23.03612965210356	22.919932409654802	32.34971261461704	21.694225323624593
18	24.307241100323623	22.546478896979504	33.383794329827396	19.76248567286947
19	25.328575883225458	25.003055049892126	30.365405036407765	19.302964030474648
20	26.72062516855448	24.604423712243797	29.86890675566343	18.806044363538295
21	25.35006657901834	25.210798442556637	31.419818635382956	18.01931634304207
22	24.4335516113808	22.71218901699029	31.664960052589	21.189299319039915
23	22.35211451591154	25.811589805825243	31.25779564455232	20.578500033710895
24	22.154379045307444	24.257201490021572	34.10626095604099	19.48215850862999
25	22.483060275080906	25.209850323624593	32.05474228020496	20.252347121089535
26	21.441288261866234	26.047039340614887	31.38695051240561	21.124721885113267
27	21.084058117583602	25.1196736785329	32.18695219795038	21.609316005933117
28	20.06714788969795	26.31419818635383	33.31363352885653	20.305020395091695
29	21.691802353020496	25.038451490021572	32.57799858414239	20.691747572815533
30	21.92925347222222	25.32109627831715	33.03141012675297	19.71824012270766
31	24.05493611785329	25.470056297195253	30.624030811758363	19.850976773193096
32	24.41985656014024	25.459205602750814	30.525005056634303	19.595932780474648
33	23.940740459816613	25.887123280744333	29.308463120280475	20.863673139158575
34	21.199833973840345	27.275380090345198	31.20238336030205	20.322402575512406
35	21.701494235436893	27.55233616504854	30.54217654395901	20.203993055555554
36	23.943584816612727	28.462951725997844	28.06716474514563	19.526298712243797
37	22.477582254584682	28.499401631607334	28.59737392125135	20.425642192556634
38	22.848507450107874	28.344858245685007	28.475698658306364	20.330935645900755
39	22.744846446871627	27.560974581984897	27.85373263888889	21.840446332254583
40	23.825596682847898	26.053676173139156	29.029400114617044	21.091327029395902
41	21.320877157497304	26.552597424487594	29.048889225997844	23.07763619201726
42	22.60347137944984	27.149596312028045	27.824656991639696	22.422275316882416
43	22.19925667475728	26.058732807443363	29.19869201725998	22.543318500539375
44	21.951270900755123	26.649937634843578	29.371249662891046	22.027541801510246
45	21.634388484358144	27.281911576321466	28.85831732065804	22.22538261866235
46	22.268996089536138	27.09692303802589	29.132955771305287	21.501125101132686
47	21.971602784519956	26.917412520226534	29.521263147249186	21.589721548004313
48	21.845397620010786	26.346539576591155	30.20285531283711	21.60520749056095
49	22.081689927184467	26.23192253236246	30.15818837648328	21.528199163969795
50	21.524301341693636	26.91973014428263	29.990265978964402	21.56570253505933
51	21.379765877831716	27.043617684735704	29.418655609492987	22.15796082793959
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	653.0
1	1062.0
2	1471.0
3	10457.5
4	19444.0
5	13485.5
6	7527.0
7	7440.5
8	7354.0
9	7480.5
10	7607.0
11	7536.0
12	7465.0
13	7293.0
14	7121.0
15	6818.5
16	6516.0
17	6252.0
18	5988.0
19	5680.5
20	5373.0
21	5410.5
22	5448.0
23	5628.5
24	5809.0
25	6478.5
26	8286.0
27	9424.0
28	11149.5
29	12875.0
30	14928.5
31	16982.0
32	19297.5
33	21613.0
34	24825.5
35	28038.0
36	30844.5
37	33651.0
38	36031.0
39	38411.0
40	42627.0
41	46843.0
42	50854.5
43	54866.0
44	56945.0
45	59024.0
46	62492.0
47	65960.0
48	67678.5
49	69397.0
50	67492.0
51	65587.0
52	62664.0
53	59741.0
54	56528.5
55	53316.0
56	49896.0
57	46476.0
58	43382.5
59	40289.0
60	37579.0
61	34869.0
62	31322.5
63	27776.0
64	24748.5
65	21721.0
66	18334.0
67	14947.0
68	12733.5
69	10520.0
70	9430.0
71	8340.0
72	6919.0
73	5498.0
74	4617.0
75	3010.0
76	2284.0
77	1720.0
78	1156.0
79	860.5
80	565.0
81	407.5
82	250.0
83	169.5
84	89.0
85	61.5
86	34.0
87	29.5
88	25.0
89	18.5
90	12.0
91	8.0
92	4.0
93	2.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	949248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.32632297399886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.58954931352574	24.230796683251043
2	7.269155513492918	4.990467598087649
3	3.9370532790810953	4.054336872707369
4	2.7976031044309053	3.8412571086302854
5	2.1328005406977493	3.660560009955518
6	1.7125899744817332	3.527214995165547
7	1.5084635627612677	3.624600521489659
8	1.2981171813111307	3.564767169902633
9	1.103151906277597	3.408043376184059
>10	7.600673015722137	42.122655634279674
>50	0.03452284429182753	0.8008719372234463
>100	0.016005922311949004	1.018697584687742
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.138416139205934E-4	1.1557305084353633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10728	1.1301577669902914	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034448321197411	0.0
2	0.0	0.0	0.0	0.0920728829557713	0.0
3	0.0	0.0	0.0	0.1700293284789644	0.0
4	0.0	0.0	0.0	0.25946854773462785	0.0
5	0.0	0.0	0.0	0.41422262675296656	0.0
6	0.0	0.0	0.0	0.827075748381877	0.0
7	0.0	0.0	0.0	1.0345031014023733	0.0
8	0.0	0.0	0.0	1.4985546453613807	0.0
9	0.0	0.0	0.0	1.7760374528047465	0.0
10	0.0	0.0	0.0	2.1659250269687162	0.0
11	0.0	0.0	0.0	2.4435131809600863	0.0
12	0.0	0.0	0.0	2.686126281014024	0.0
13	0.0	0.0	0.0	2.834664913700108	0.0
14	0.0	0.0	0.0	2.9275805690399137	0.0
15	0.0	0.0	0.0	3.0003750337108954	0.0
16	0.0	0.0	0.0	3.1145706917475726	0.0
17	0.0	0.0	0.0	3.232348132416397	0.0
18	0.0	0.0	0.0	3.3836257753505934	0.0
19	0.0	0.0	0.0	3.4732756877022655	0.0
20	0.0	0.0	0.0	3.5599758967098167	0.0
21	0.0	0.0	0.0	3.6742769012944985	0.0
22	0.0	0.0	0.0	3.792897114347357	0.0
23	0.0	0.0	0.0	3.9206824770765913	0.0
24	0.0	0.0	0.0	4.033297936893204	0.0
25	0.0	0.0	0.0	4.136432207389428	0.0
26	0.0	0.0	0.0	4.241568062297735	0.0
27	0.0	0.0	0.0	4.356817185814455	0.0
28	0.0	0.0	0.0	4.47290908171521	0.0
29	0.0	0.0	0.0	4.594900384304207	0.0
30	0.0	0.0	0.0	4.758503573354909	0.0
31	0.0	0.0	0.0	4.880810915587918	0.0
32	0.0	0.0	0.0	5.014706378101402	0.0
33	0.0	0.0	0.0	5.1536584749190935	0.0
34	0.0	0.0	0.0	5.289766214940669	0.0
35	0.0	0.0	0.0	5.43798880798274	0.0
36	0.0	0.0	0.0	5.569250606796117	0.0
37	0.0	0.0	0.0	5.712205872437972	0.0
38	0.0	0.0	0.0	5.88476351806904	0.0
39	0.0	0.0	0.0	6.123373449298813	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	30	2.1651013E-6	45.000004	21
CGTTTTT	6975	0.0	42.322582	1
ACGTTAG	65	0.0	41.53846	1
GCGATCG	110	0.0	40.909092	9
CGTTAGG	165	0.0	39.545456	2
CACAACG	230	0.0	39.130436	12
GGGCGAT	2230	0.0	38.643494	7
CTATGCG	35	6.247137E-6	38.57143	1
CGAGGGT	350	0.0	38.571426	4
GCCGATT	65	9.094947E-12	38.07692	9
GCGATAT	130	0.0	38.07692	9
ATTGGGC	315	0.0	37.857143	4
ACAACGA	245	0.0	37.65306	13
TCTAGCG	30	1.139772E-4	37.500004	1
CTACGAA	30	1.139772E-4	37.500004	11
TATAGCG	30	1.139772E-4	37.500004	1
AGGGTAC	655	0.0	37.44275	6
AAGGGTA	795	0.0	37.35849	5
AGGGCGA	1240	0.0	37.197582	6
GGCGATA	480	0.0	37.031254	8
>>END_MODULE