FastQCFastQC Report
Thu 26 May 2016
SRR1547095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547095_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1054398
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121701.1542131149717658No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT207.0330204E-445.00000439
CATAGCG351.2117926E-745.0000041
ATACCGG207.0330204E-445.0000042
CGTTTTT78600.042.8244251
ATAGCGG2000.041.6252
GTCGATG403.4578625E-739.3750041
GCGATAC800.039.3750049
TATAGCG800.039.3750041
GGGCGAT22400.038.671887
GTTTGCG700.038.571431
TGTACGG1050.038.571432
AGGGCGA11650.037.6609466
TATGCGA301.1398578E-437.5000042
TGTAGCG601.546141E-1037.5000041
GGCGATA5000.036.98
GCCCTAC4400.036.8181826
TAGGGCG4950.036.818185
ACGGGCT1350.036.6666685
TAAGGGA10700.036.5887834
GGTACCT5200.036.3461578
Produced by FastQC (version 0.11.5)