FastQCFastQC Report
Thu 26 May 2016
SRR1547094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547094_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences768589
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102251.330359919280656No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA30700.399433247158104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT600.045.00000414
TAATACG207.031826E-445.01
AACGGGC700.045.04
ATATCGG207.031826E-445.03
GAAACGT207.031826E-445.09
TACGGGA2150.043.9534874
CGTTTTT63350.042.655881
CATTAGA5850.042.3076917
GCATTAG5850.042.3076916
TATGCGG700.041.7857132
TGATTCG603.6379788E-1241.25000415
CTTGACC6500.041.192317
TGCATTA6050.040.90909215
CTCAACT6550.040.534352
ACTTGAC6650.040.2631576
TCAACTT6600.040.2272723
TTGACCC6600.039.2045448
ACCCTGC6650.038.9097711
TATACTA2550.038.8235344
GCTCAAC7050.038.6170231
Produced by FastQC (version 0.11.5)