##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547094_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	768589
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42795434230779	31.0	31.0	33.0	30.0	34.0
2	31.79396660633967	31.0	31.0	34.0	30.0	34.0
3	31.830423021927192	31.0	31.0	34.0	30.0	34.0
4	35.65148473371334	37.0	35.0	37.0	33.0	37.0
5	35.61015835511567	37.0	35.0	37.0	33.0	37.0
6	35.65427426101596	37.0	35.0	37.0	33.0	37.0
7	35.991532535594445	37.0	35.0	37.0	35.0	37.0
8	34.67687411607504	37.0	35.0	37.0	33.0	37.0
9	36.99313156966858	39.0	37.0	39.0	34.0	39.0
10	36.912027104213045	39.0	37.0	39.0	32.0	39.0
11	36.85029710287293	39.0	37.0	39.0	32.0	39.0
12	36.68016846454997	39.0	35.0	39.0	32.0	39.0
13	36.67215768115339	39.0	35.0	39.0	32.0	39.0
14	37.79704107136584	40.0	36.0	41.0	33.0	41.0
15	37.909236275824924	40.0	36.0	41.0	33.0	41.0
16	37.9278444005834	40.0	36.0	41.0	33.0	41.0
17	37.790905152168456	40.0	36.0	41.0	33.0	41.0
18	37.6513181947699	39.0	36.0	41.0	33.0	41.0
19	37.51682628817222	39.0	36.0	41.0	32.0	41.0
20	37.30391665766749	39.0	35.0	41.0	32.0	41.0
21	37.18697379223486	39.0	35.0	41.0	32.0	41.0
22	37.09374190887458	39.0	35.0	41.0	32.0	41.0
23	36.98375985084356	39.0	35.0	41.0	31.0	41.0
24	36.79582065317094	38.0	35.0	41.0	31.0	41.0
25	36.677764058554054	38.0	35.0	41.0	31.0	41.0
26	36.57609463575461	38.0	35.0	40.0	31.0	41.0
27	36.47683872654956	38.0	35.0	40.0	31.0	41.0
28	36.4843889256807	38.0	35.0	40.0	31.0	41.0
29	36.426580396024406	38.0	35.0	40.0	30.0	41.0
30	36.349279003472596	38.0	35.0	40.0	30.0	41.0
31	36.21510586282135	38.0	35.0	40.0	30.0	41.0
32	36.01630780560222	38.0	35.0	41.0	30.0	41.0
33	35.728389295188975	38.0	35.0	41.0	27.0	41.0
34	35.40815051997882	38.0	35.0	41.0	25.0	41.0
35	35.17256557145627	38.0	34.0	41.0	24.0	41.0
36	34.98498287120945	38.0	34.0	40.0	23.0	41.0
37	34.92351438805395	38.0	34.0	40.0	23.0	41.0
38	34.77796585691442	38.0	34.0	40.0	23.0	41.0
39	34.64351168179612	38.0	34.0	40.0	23.0	41.0
40	34.481032125101976	37.0	34.0	40.0	22.0	41.0
41	34.33375445133875	37.0	33.0	40.0	22.0	41.0
42	34.23202647969201	37.0	33.0	40.0	22.0	41.0
43	34.11181398640886	37.0	33.0	40.0	21.0	41.0
44	33.968293847557014	37.0	33.0	40.0	21.0	41.0
45	33.82721714726596	36.0	33.0	40.0	20.0	41.0
46	33.75257517346722	36.0	33.0	40.0	20.0	41.0
47	33.67217979960681	36.0	33.0	40.0	20.0	41.0
48	33.59046642613933	35.0	33.0	40.0	20.0	41.0
49	33.49535837749434	35.0	33.0	40.0	20.0	41.0
50	33.40451919036052	35.0	33.0	40.0	20.0	41.0
51	33.05470413966372	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	8.0
14	9.0
15	23.0
16	65.0
17	149.0
18	366.0
19	800.0
20	1307.0
21	2079.0
22	3188.0
23	4650.0
24	7148.0
25	10744.0
26	15518.0
27	17708.0
28	18098.0
29	18068.0
30	19610.0
31	22694.0
32	27164.0
33	34313.0
34	52209.0
35	69718.0
36	66469.0
37	86324.0
38	120743.0
39	169304.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.074388262127094	22.490824094542074	24.919950714881427	18.514836928449405
2	36.40176999670825	23.554461487218788	25.95275238131173	14.091016134761231
3	28.670329656032028	24.341878429173462	34.024947013293186	12.962844901501322
4	24.72439756488839	26.932339650970803	32.64345443403431	15.699808350106496
5	22.183117374825816	31.323112873070002	31.911333625643874	14.582436126460305
6	20.713801524611984	39.29863685272623	29.77365015632542	10.213911466336365
7	82.86587499951209	3.3199798591965277	11.353532251957809	2.460612889333571
8	80.114599610455	5.805963915694864	11.57523722041299	2.5041992534371427
9	77.80712448395697	4.576828447974145	14.203950355781828	3.4120967122870614
10	40.25662610315786	27.578979142298422	19.026814070979416	13.137580683564298
11	30.77795805040145	27.218578460009184	26.53811074579522	15.465352743794147
12	26.530304232821443	21.743870911501464	31.035052544337745	20.69077231133935
13	24.350855919093302	23.61821467650461	31.936444575709515	20.094484828692575
14	22.980162349448143	24.00320587466123	32.685219278444	20.331412497446617
15	21.842623300619707	21.258956347280535	35.809906204746625	21.088514147353138
16	25.029632222162952	20.57406494238143	34.622145255786904	19.774157579668717
17	23.914601952408894	20.946695828329574	31.87386236336976	23.26483985589177
18	24.873111637038782	22.01957092802525	32.02218610987146	21.085131325064502
19	26.883418836335153	25.298566594109467	28.873038776251025	18.94497579330435
20	29.38423526748366	24.322752472387712	28.15132665182562	18.141685608303007
21	27.998188888990082	25.455347396332762	28.441859042999575	18.104604671677578
22	27.45888895105186	22.550543918791448	28.576131066148484	21.414436064008203
23	26.167821813739202	25.75810999116563	27.857151221263898	20.216916973831268
24	23.911479347219387	25.200334639189474	31.37918965793161	19.508996355659527
25	24.20292249824028	24.996974976222663	29.234090001288077	21.566012524248983
26	23.11859784618307	24.658952964458248	29.68738818796522	22.535061001393466
27	22.865406608733664	22.84042576721759	30.203528804081248	24.0906388199675
28	22.370603794745954	24.244817451199534	31.21304104013979	22.17153771391472
29	22.910554275431995	24.12837029934074	30.91639354713638	22.04468187809089
30	23.027391752939476	23.744940403778873	31.060553820052068	22.16711402322958
31	24.82874462163783	23.893914693028393	28.412324402248796	22.865016283084977
32	25.408898644138805	24.710345841535595	27.493237608136468	22.38751790618913
33	24.763430129757253	24.347212879705538	25.976952571530425	24.91240441900678
34	22.763531614425915	27.974509132969636	28.100584317496086	21.16137493510836
35	24.264463842183535	27.755016009857027	26.844126054367155	21.136394093592283
36	25.37507042125245	28.780401488962244	24.36920122458167	21.47532686520364
37	23.94856028384481	28.383049978597143	24.24780994783948	23.420579789718563
38	25.730266761559168	27.031612474287297	25.070616415275264	22.16750434887827
39	24.430612459975357	26.387184828302253	25.053311978183395	24.12889073353899
40	26.26149996942449	24.118742266673085	27.62881071678101	21.990947047121416
41	23.687172207772946	25.107957568999815	27.25839167617543	23.946478547051804
42	25.00803420293551	24.896661284509666	25.618763734583766	24.476540777971064
43	23.625370646730566	24.266805796075666	27.669664801343764	24.438158755850008
44	24.44102114394039	24.739750373736808	27.837895155928592	22.98133332639421
45	23.294114279543425	24.51049910940698	27.025887698106533	25.169498912943066
46	23.847205723735314	24.654008839574857	27.779216200075723	23.719569236614106
47	23.75378778514915	25.198903445144282	28.5112068999166	22.536101869789967
48	23.968857217576627	24.516353994137308	27.998579214638774	23.51620957364729
49	23.93008486980688	24.87220087719184	27.59368140839903	23.60403284460225
50	22.688719198427247	25.07412934611346	28.835567513976912	23.40158394148238
51	22.941520110227962	24.718542680158055	27.790925969536385	24.549011240077597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	633.0
1	963.0
2	1293.0
3	9116.5
4	16940.0
5	11598.0
6	6256.0
7	6283.5
8	6311.0
9	6403.0
10	6495.0
11	6579.0
12	6663.0
13	6593.5
14	6524.0
15	6174.5
16	5825.0
17	5506.5
18	5188.0
19	4962.0
20	4736.0
21	4566.5
22	4397.0
23	4459.5
24	4522.0
25	4587.5
26	5166.0
27	5679.0
28	6266.5
29	6854.0
30	9271.5
31	11689.0
32	11439.5
33	11190.0
34	12681.0
35	14172.0
36	15715.5
37	17259.0
38	19214.5
39	21170.0
40	23555.5
41	25941.0
42	28231.5
43	30522.0
44	33796.0
45	37070.0
46	41074.0
47	45078.0
48	47869.0
49	50660.0
50	51029.5
51	51399.0
52	49398.0
53	47397.0
54	45840.5
55	44284.0
56	43043.0
57	41802.0
58	41778.5
59	41755.0
60	41394.0
61	41033.0
62	40406.5
63	39780.0
64	36590.0
65	33400.0
66	28983.5
67	24567.0
68	20121.5
69	15676.0
70	13292.0
71	10908.0
72	9160.5
73	7413.0
74	6313.5
75	4170.0
76	3126.0
77	2391.0
78	1656.0
79	1242.0
80	828.0
81	580.0
82	332.0
83	249.5
84	167.0
85	119.5
86	72.0
87	50.5
88	29.0
89	22.5
90	16.0
91	13.5
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	768589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.69933052920877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.27408489354725	26.42161377874645
2	8.243567189611559	6.380410628210368
3	4.9179735142280325	5.709668476830152
4	3.622040345139574	5.606821460267432
5	2.847583589259308	5.509978926514832
6	2.378829709542061	5.523547032136197
7	1.963208557251124	5.318239979838264
8	1.6072627056442867	4.975999255439892
9	1.2726782737947606	4.432661745743368
>10	4.8165852851413025	25.75974970894479
>50	0.03186155747326736	0.8499886795822698
>100	0.023296588690048702	1.6556928051994209
>500	3.425968925004655E-4	0.0929404753718988
>1k	3.425968925004655E-4	0.40702890070146835
>5k	0.0	0.0
>10k+	3.425968925004655E-4	1.3556581464731317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10225	1.330359919280656	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	3070	0.399433247158104	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02784322960646067	0.0
2	0.0	0.0	0.0	0.08912435645058672	0.0
3	0.0	0.0	0.0	0.17239382817084292	0.0
4	0.0	0.0	0.0	0.29794857849904177	0.0
5	0.0	0.0	0.0	0.5166610503142772	0.0
6	0.0	0.0	0.0	1.1298626444042263	0.0
7	0.0	0.0	0.0	1.4574759722036095	0.0
8	0.0	0.0	0.0	2.254781163925063	0.0
9	0.0	0.0	0.0	2.7352720374608537	0.0
10	0.0	0.0	0.0	3.3422284211717836	0.0
11	0.0	0.0	0.0	3.717721695210314	0.0
12	0.0	0.0	0.0	3.974816189146605	0.0
13	0.0	0.0	0.0	4.166986516851009	0.0
14	0.0	0.0	0.0	4.264828146122309	0.0
15	0.0	0.0	0.0	4.367353683177876	0.0
16	0.0	0.0	0.0	4.470399654431692	0.0
17	0.0	0.0	0.0	4.569542369198622	0.0
18	0.0	0.0	0.0	4.7200779610428985	0.0
19	0.0	0.0	0.0	4.806209820853538	0.0
20	0.0	0.0	0.0	4.89871699959276	0.0
21	0.0	0.0	0.0	5.002413513594392	0.0
22	0.0	0.0	0.0	5.124845658733081	0.0
23	0.0	0.0	0.0	5.248058455169148	0.0
24	0.0	0.0	0.0	5.368799189163519	0.0
25	0.0	0.0	0.0	5.500469041321174	0.0
26	0.0	0.0	0.0	5.595578391051655	0.0
27	0.0	0.0	0.0	5.704088921387113	0.0
28	0.0	0.0	0.0	5.807004784091367	0.0
29	0.0	0.0	0.0	5.917597051219833	0.0
30	0.0	0.0	0.0	6.07359720214575	0.0
31	0.0	0.0	0.0	6.202274557663459	0.0
32	0.0	0.0	0.0	6.370765129347414	0.0
33	0.0	0.0	0.0	6.500483353261626	0.0
34	0.0	0.0	0.0	6.6700147933420855	0.0
35	0.0	0.0	0.0	6.819509516789858	0.0
36	0.0	0.0	0.0	6.941551336279859	0.0
37	0.0	0.0	0.0	7.085581500645989	0.0
38	0.0	0.0	0.0	7.258235545915958	0.0
39	0.0	0.0	0.0	7.511426783365362	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	60	0.0	45.000004	14
TAATACG	20	7.031826E-4	45.0	1
AACGGGC	70	0.0	45.0	4
ATATCGG	20	7.031826E-4	45.0	3
GAAACGT	20	7.031826E-4	45.0	9
TACGGGA	215	0.0	43.953487	4
CGTTTTT	6335	0.0	42.65588	1
CATTAGA	585	0.0	42.30769	17
GCATTAG	585	0.0	42.30769	16
TATGCGG	70	0.0	41.785713	2
TGATTCG	60	3.6379788E-12	41.250004	15
CTTGACC	650	0.0	41.19231	7
TGCATTA	605	0.0	40.909092	15
CTCAACT	655	0.0	40.53435	2
ACTTGAC	665	0.0	40.263157	6
TCAACTT	660	0.0	40.227272	3
TTGACCC	660	0.0	39.204544	8
ACCCTGC	665	0.0	38.90977	11
TATACTA	255	0.0	38.82353	44
GCTCAAC	705	0.0	38.617023	1
>>END_MODULE