FastQCFastQC Report
Thu 26 May 2016
SRR1547093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547093_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences618894
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.6427595032428817No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG207.03076E-445.02
TATACGG603.6379788E-1241.2499962
GCGATTC950.040.2631579
TACGGGC850.039.7058834
CGGGTAT356.2436156E-638.571436
CGCATCG356.2436156E-638.5714321
TTCGTAC356.2436156E-638.5714333
CATCGTT356.2436156E-638.5714336
ATACCGG356.2436156E-638.571432
CGTTTTT22450.038.4855231
GTTGATC3700.037.702716
ATCTACG301.13931324E-437.4999961
TCGATAG301.13931324E-437.4999961
GTATGCG301.13931324E-437.4999961
GTAGGGA6800.037.0588234
TAGGGCG2500.036.8999985
CGAATAT552.744855E-936.81818414
CCGAACG552.744855E-936.81818436
CGTCATA1100.036.81818438
GTAAGGG7500.036.63
Produced by FastQC (version 0.11.5)