##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547093_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	618894
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.379005128503426	31.0	31.0	33.0	30.0	34.0
2	31.74311917711272	31.0	31.0	34.0	30.0	34.0
3	31.84560199323309	31.0	31.0	34.0	30.0	34.0
4	35.61860674041112	37.0	35.0	37.0	33.0	37.0
5	35.55053046240552	37.0	35.0	37.0	33.0	37.0
6	35.588931222471054	37.0	35.0	37.0	33.0	37.0
7	35.95388224801016	37.0	35.0	37.0	35.0	37.0
8	34.60777612967649	37.0	35.0	37.0	33.0	37.0
9	36.91702617895795	39.0	37.0	39.0	33.0	39.0
10	36.82959602128959	39.0	37.0	39.0	32.0	39.0
11	36.748152349190654	39.0	35.0	39.0	32.0	39.0
12	36.4624653010047	39.0	35.0	39.0	32.0	39.0
13	36.40376381092724	39.0	35.0	39.0	32.0	39.0
14	37.49396019350648	40.0	36.0	41.0	32.0	41.0
15	37.640353598516064	40.0	36.0	41.0	32.0	41.0
16	37.666304730697014	40.0	36.0	41.0	33.0	41.0
17	37.559173299466465	40.0	35.0	41.0	32.0	41.0
18	37.47704938163886	39.0	36.0	41.0	32.0	41.0
19	37.42041124974551	39.0	36.0	41.0	32.0	41.0
20	37.28883944585018	39.0	35.0	41.0	32.0	41.0
21	37.183540315465976	39.0	35.0	41.0	32.0	41.0
22	37.087898735486206	39.0	35.0	41.0	32.0	41.0
23	37.002943961324554	39.0	35.0	41.0	31.0	41.0
24	36.85752487501899	39.0	35.0	41.0	31.0	41.0
25	36.73561870045597	38.0	35.0	41.0	31.0	41.0
26	36.602117648579565	38.0	35.0	41.0	31.0	41.0
27	36.49908384957683	38.0	35.0	41.0	30.0	41.0
28	36.516880111941624	38.0	35.0	41.0	31.0	41.0
29	36.469616767976426	38.0	35.0	41.0	30.0	41.0
30	36.3918134607865	38.0	35.0	40.0	30.0	41.0
31	36.315839222871766	38.0	35.0	40.0	30.0	41.0
32	36.23022359240839	38.0	35.0	41.0	30.0	41.0
33	36.086030887357126	38.0	35.0	41.0	30.0	41.0
34	35.92472701302646	38.0	35.0	41.0	29.0	41.0
35	35.78583570045921	38.0	35.0	41.0	29.0	41.0
36	35.6542687439206	38.0	35.0	41.0	28.0	41.0
37	35.610419554883386	38.0	35.0	40.0	28.0	41.0
38	35.48476475777758	38.0	35.0	40.0	27.0	41.0
39	35.376642849987235	38.0	34.0	40.0	27.0	41.0
40	35.22290569952205	38.0	34.0	40.0	26.0	41.0
41	35.10609732846012	38.0	34.0	40.0	26.0	41.0
42	35.00883026818809	37.0	34.0	40.0	26.0	41.0
43	34.919404615329924	37.0	34.0	40.0	26.0	41.0
44	34.77463669061261	37.0	34.0	40.0	25.0	41.0
45	34.64998529635123	37.0	34.0	40.0	24.0	41.0
46	34.575163113554176	37.0	34.0	40.0	24.0	41.0
47	34.491615688631654	36.0	34.0	40.0	24.0	41.0
48	34.37366010980879	36.0	33.0	40.0	24.0	41.0
49	34.29433957996038	36.0	33.0	40.0	24.0	41.0
50	34.18469560215481	36.0	33.0	40.0	24.0	41.0
51	33.803415447556446	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	1.0
13	3.0
14	7.0
15	31.0
16	61.0
17	164.0
18	295.0
19	563.0
20	1003.0
21	1594.0
22	2415.0
23	3313.0
24	4848.0
25	6783.0
26	8795.0
27	10766.0
28	11633.0
29	12987.0
30	15261.0
31	18319.0
32	22379.0
33	29038.0
34	45668.0
35	63166.0
36	52311.0
37	68399.0
38	94121.0
39	144876.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.427553022003764	21.609031595071208	27.26234217814359	15.701073204781432
2	33.895788293310325	24.253103116204066	28.719134455981155	13.131974134504453
3	30.15265942148413	25.362986230275297	30.92807492074572	13.556279427494855
4	26.11691178133897	28.195135192779375	30.48211810099952	15.205834924882128
5	23.523737505938012	31.79510546232473	29.81479865695903	14.866358374778233
6	21.688366666989825	40.51824706654128	26.959059225004605	10.83432704146429
7	85.90033188235789	4.091007506939799	7.922843007041594	2.0858176036607237
8	82.76231470978875	6.541184758617792	7.450387303803237	3.246113227790219
9	79.78862293058262	5.547315049103724	10.716697851328338	3.9473641689853194
10	43.69520467155927	25.409520854944468	16.520438071786124	14.374836401710148
11	33.65261256370234	25.66836970466671	24.074881966863472	16.604135764767474
12	29.252990011213555	22.10491618920203	28.66839878880713	19.97369501077729
13	24.08683878014652	25.613432994987832	31.06315459513261	19.23657362973304
14	21.727791835112313	27.65610912369485	31.091753999877202	19.524345041315637
15	20.391052425778888	23.252447107259076	36.671384760556734	19.6851157064053
16	23.226756116556306	21.466034571348246	34.84263864248159	20.46457066961386
17	23.600164163814803	22.605001825837704	28.85922306566229	24.935610944685198
18	24.38075017692852	22.598377104964662	30.963137467805474	22.057735250301345
19	27.35815826296587	25.063581162525406	26.48628036465049	21.091980209858228
20	28.483714497151368	24.23209790367979	25.552033142993793	21.73215445617505
21	26.562383865411526	25.372034629516527	27.64576809599059	20.41981340908136
22	26.196408431815467	23.095554327558514	27.181068163530426	23.526969077095593
23	24.561718161753063	25.792946772791463	26.12337492365413	23.521960141801344
24	23.795835797406344	23.786625819607234	30.92209651410419	21.49544186888223
25	24.12771815528992	24.1278797338478	28.575975853700307	23.16842625716197
26	22.066622070984693	26.87891626029659	27.19997285480228	23.85448881391644
27	22.563960872136423	25.230653391372353	27.827705552162406	24.377680184328817
28	22.466367423177473	25.522464266901924	29.986071928310825	22.025096381609774
29	23.371368925858064	23.675621350344326	29.459648986740866	23.49336073705675
30	23.83833095812853	24.4287390086186	28.904949797542066	22.8279802357108
31	26.04307038038824	24.455561049226525	25.73574796330228	23.76562060708296
32	26.66934887072746	24.023661564015807	25.83027141966153	23.476718145595207
33	25.64526397089001	24.065187253390725	26.218706272802773	24.07084250291649
34	23.207043532495064	25.14162360598099	28.143591632815955	23.507741228707985
35	23.77563847767146	25.18007930275621	28.434109879882502	22.610172339689832
36	26.177342161985734	24.97762136973375	26.838036885153194	22.00699958312732
37	24.66383581033263	27.14148141685006	26.60908006863857	21.585602704178743
38	24.398685396853097	26.77647545460127	26.53701603182451	22.28782311672112
39	23.76562060708296	26.60019324795522	26.30466606559443	23.329520079367388
40	25.011391288330476	24.336639230627537	27.93483213603622	22.71713734500577
41	22.651213293391116	24.236460524742526	28.04890659789883	25.063419583967526
42	24.371701777687292	25.84788348247034	25.98296315685723	23.797451582985133
43	23.443594541230002	24.09863401487169	28.474019783678628	23.98375166021968
44	23.478333931173996	25.404835076765973	27.573865637734414	23.54296535432562
45	22.49787524196389	26.10883285344502	27.113366747779104	24.27992515681199
46	24.045959405003117	25.504690625535233	27.300959453476686	23.148390515984968
47	23.849964614295825	25.042575950001133	27.809285596564195	23.29817383913885
48	23.4814039237737	24.342617637269065	28.642061483872844	23.53391695508439
49	23.553791117703515	23.770629542377208	28.412619931684585	24.26295940823469
50	22.607748661321647	25.07489166157694	29.00529008198496	23.31206959511645
51	22.02768163853584	26.55430493751757	27.589861914964438	23.828151508982153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	581.0
1	803.0
2	1025.0
3	3939.5
4	6854.0
5	4823.0
6	2792.0
7	2798.5
8	2805.0
9	2890.0
10	2975.0
11	3104.0
12	3233.0
13	3199.0
14	3165.0
15	3167.0
16	3169.0
17	2981.5
18	2794.0
19	2759.5
20	2725.0
21	2900.0
22	3075.0
23	3168.0
24	3261.0
25	3399.0
26	3905.5
27	4274.0
28	5058.5
29	5843.0
30	6965.5
31	8088.0
32	9165.5
33	10243.0
34	12135.0
35	14027.0
36	15082.0
37	16137.0
38	17721.5
39	19306.0
40	22622.0
41	25938.0
42	29312.5
43	32687.0
44	34913.0
45	37139.0
46	40699.0
47	44259.0
48	46092.5
49	47926.0
50	46074.0
51	44222.0
52	41603.5
53	38985.0
54	36622.5
55	34260.0
56	33456.0
57	32652.0
58	31675.0
59	30698.0
60	30300.0
61	29902.0
62	27706.5
63	25511.0
64	23873.5
65	22236.0
66	19371.0
67	16506.0
68	14325.0
69	12144.0
70	10540.5
71	8937.0
72	7538.5
73	6140.0
74	4922.0
75	3053.0
76	2402.0
77	1932.5
78	1463.0
79	1062.5
80	662.0
81	493.0
82	324.0
83	235.5
84	147.0
85	118.5
86	90.0
87	62.5
88	35.0
89	20.5
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	618894.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.58442393652965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63559260394837	24.058401109555298
2	6.790435079455799	4.561057008438519
3	3.574314168265342	3.6012384692810278
4	2.507701704122493	3.3687886855043057
5	1.9248802200046926	3.2322996667839026
6	1.5738688742186213	3.1714487695360103
7	1.3635217008713043	3.20551635941039
8	1.1700664726686218	3.143680676161831
9	1.072722318787489	3.2424085008302526
>10	8.338968069646297	45.93080705673637
>50	0.03014078421340916	0.6714110654800207
>100	0.01679978136439883	1.0505940324922505
>500	4.941112165999656E-4	0.10222175391174007
>1k	4.941112165999656E-4	0.6601268458780876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3978	0.6427595032428817	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.615785578790552E-4	0.0	0.0	0.04621146755340979	0.0
2	1.615785578790552E-4	0.0	0.0	0.13895755977598748	0.0
3	1.615785578790552E-4	0.0	0.0	0.23752048008221116	0.0
4	1.615785578790552E-4	0.0	0.0	0.4792420026692778	0.0
5	1.615785578790552E-4	0.0	0.0	0.8040149040061788	0.0
6	1.615785578790552E-4	0.0	0.0	1.7017453715822095	0.0
7	1.615785578790552E-4	0.0	0.0	2.1024601951222666	0.0
8	1.615785578790552E-4	0.0	0.0	3.1328466587170016	0.0
9	1.615785578790552E-4	0.0	0.0	3.640849644688751	0.0
10	1.615785578790552E-4	0.0	0.0	4.265997085122816	0.0
11	1.615785578790552E-4	0.0	0.0	4.700643405817474	0.0
12	1.615785578790552E-4	0.0	0.0	5.0599941185404935	0.0
13	1.615785578790552E-4	0.0	0.0	5.274570443403879	0.0
14	1.615785578790552E-4	0.0	0.0	5.384928598435273	0.0
15	1.615785578790552E-4	0.0	0.0	5.468141555742987	0.0
16	1.615785578790552E-4	0.0	0.0	5.599181766182901	0.0
17	1.615785578790552E-4	0.0	0.0	5.72230462728674	0.0
18	1.615785578790552E-4	0.0	0.0	5.9228236176146485	0.0
19	1.615785578790552E-4	0.0	0.0	6.028980730141187	0.0
20	1.615785578790552E-4	0.0	0.0	6.138369413825307	0.0
21	1.615785578790552E-4	0.0	0.0	6.276034345138263	0.0
22	1.615785578790552E-4	0.0	0.0	6.43728974590156	0.0
23	1.615785578790552E-4	0.0	0.0	6.59983777512789	0.0
24	1.615785578790552E-4	0.0	0.0	6.748974784050257	0.0
25	1.615785578790552E-4	0.0	0.0	6.864503452933782	0.0
26	1.615785578790552E-4	0.0	0.0	6.986979999806105	0.0
27	1.615785578790552E-4	0.0	0.0	7.118020210246019	0.0
28	1.615785578790552E-4	0.0	0.0	7.253907777422305	0.0
29	1.615785578790552E-4	0.0	0.0	7.379131159778573	0.0
30	3.231571157581104E-4	0.0	0.0	7.561553351624026	0.0
31	3.231571157581104E-4	0.0	0.0	7.696309868895158	0.0
32	3.231571157581104E-4	0.0	0.0	7.840437942523275	0.0
33	3.231571157581104E-4	0.0	0.0	7.976810245373198	0.0
34	3.231571157581104E-4	0.0	0.0	8.117060433612219	0.0
35	3.231571157581104E-4	0.0	0.0	8.266682178208223	0.0
36	3.231571157581104E-4	0.0	0.0	8.410325516162704	0.0
37	4.8473567363716566E-4	0.0	0.0	8.561724624895378	0.0
38	4.8473567363716566E-4	0.0	0.0	8.71716319757503	0.0
39	4.8473567363716566E-4	0.0	0.0	8.942565285816311	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	20	7.03076E-4	45.0	2
TATACGG	60	3.6379788E-12	41.249996	2
GCGATTC	95	0.0	40.263157	9
TACGGGC	85	0.0	39.705883	4
CGGGTAT	35	6.2436156E-6	38.57143	6
CGCATCG	35	6.2436156E-6	38.57143	21
TTCGTAC	35	6.2436156E-6	38.57143	33
CATCGTT	35	6.2436156E-6	38.57143	36
ATACCGG	35	6.2436156E-6	38.57143	2
CGTTTTT	2245	0.0	38.485523	1
GTTGATC	370	0.0	37.7027	16
ATCTACG	30	1.13931324E-4	37.499996	1
TCGATAG	30	1.13931324E-4	37.499996	1
GTATGCG	30	1.13931324E-4	37.499996	1
GTAGGGA	680	0.0	37.058823	4
TAGGGCG	250	0.0	36.899998	5
CGAATAT	55	2.744855E-9	36.818184	14
CCGAACG	55	2.744855E-9	36.818184	36
CGTCATA	110	0.0	36.818184	38
GTAAGGG	750	0.0	36.6	3
>>END_MODULE