FastQCFastQC Report
Thu 26 May 2016
SRR1547092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547092_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences802382
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.5497381546445458No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8340.10394051711030408No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC207.0320116E-445.019
CGATATA253.8897495E-545.010
TATCGAC207.0320116E-445.015
TAGTACG207.0320116E-445.01
TAGACGG650.044.9999962
TTAACGG302.1646792E-644.9999962
CGGTCTA650.041.5384631
TCACGAC603.6379788E-1241.24999625
TAGCATA4800.040.78124630
ATGGGTA2100.040.7142875
AACACGT3900.040.38461741
CGTTTTT26600.040.0939871
CGTTCGG451.9270374E-840.045
ACACGAC4100.039.51219626
AATGCGG1150.039.1304322
TAGGGAC6450.039.069775
TTGATCC5250.039.017
ACACGTG4100.038.96341342
CGAGACA4050.038.8888922
AGGGTAC3550.038.661976
Produced by FastQC (version 0.11.5)