##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547092_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	802382
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389724595018333	31.0	31.0	33.0	30.0	34.0
2	31.752361094840115	31.0	31.0	34.0	30.0	34.0
3	31.871977437180796	31.0	31.0	34.0	30.0	34.0
4	35.626731407234956	37.0	35.0	37.0	33.0	37.0
5	35.56151309476035	37.0	35.0	37.0	33.0	37.0
6	35.6050808717045	37.0	35.0	37.0	33.0	37.0
7	35.959344800855455	37.0	35.0	37.0	35.0	37.0
8	34.63479240561229	37.0	35.0	37.0	33.0	37.0
9	36.94510594704268	39.0	37.0	39.0	33.0	39.0
10	36.8321535129153	39.0	37.0	39.0	32.0	39.0
11	36.75739859568136	39.0	35.0	39.0	32.0	39.0
12	36.3182224925285	38.0	35.0	39.0	32.0	39.0
13	36.142192870727406	38.0	35.0	39.0	32.0	39.0
14	37.17037520781872	39.0	35.0	41.0	32.0	41.0
15	37.38011196661939	39.0	35.0	41.0	32.0	41.0
16	37.4586094902428	39.0	35.0	41.0	32.0	41.0
17	37.36726272523561	39.0	35.0	41.0	32.0	41.0
18	37.323835778968125	39.0	35.0	41.0	32.0	41.0
19	37.259742865617625	39.0	35.0	41.0	32.0	41.0
20	37.12704173323928	39.0	35.0	41.0	32.0	41.0
21	37.00041875316246	39.0	35.0	41.0	32.0	41.0
22	36.90927264071228	39.0	35.0	41.0	31.0	41.0
23	36.86023614687269	38.0	35.0	41.0	31.0	41.0
24	36.69896508146992	38.0	35.0	41.0	31.0	41.0
25	36.60128218230219	38.0	35.0	41.0	31.0	41.0
26	36.49321769431518	38.0	35.0	40.0	31.0	41.0
27	36.39304720195618	38.0	35.0	40.0	31.0	41.0
28	36.415973937600796	38.0	35.0	40.0	31.0	41.0
29	36.37695885500921	38.0	35.0	40.0	31.0	41.0
30	36.314947244579265	38.0	35.0	40.0	30.0	41.0
31	36.22606314697987	38.0	35.0	40.0	30.0	41.0
32	36.114047922311315	38.0	35.0	40.0	30.0	41.0
33	35.98821135070328	38.0	35.0	41.0	30.0	41.0
34	35.85013871198507	38.0	35.0	41.0	29.0	41.0
35	35.73864942134794	38.0	35.0	41.0	29.0	41.0
36	35.6167984825183	38.0	35.0	40.0	29.0	41.0
37	35.55018681874718	38.0	35.0	40.0	28.0	41.0
38	35.45067561336122	38.0	34.0	40.0	28.0	41.0
39	35.365922964373574	38.0	34.0	40.0	27.0	41.0
40	35.19118699073508	37.0	34.0	40.0	27.0	41.0
41	35.094641953583206	37.0	34.0	40.0	26.0	41.0
42	35.02851385001159	37.0	34.0	40.0	26.0	41.0
43	34.928630004162606	37.0	34.0	40.0	26.0	41.0
44	34.83043114127685	37.0	34.0	40.0	26.0	41.0
45	34.71901039654429	37.0	34.0	40.0	25.0	41.0
46	34.64333197903243	36.0	34.0	40.0	25.0	41.0
47	34.56001256259487	36.0	34.0	40.0	25.0	41.0
48	34.48902642382307	36.0	34.0	40.0	24.0	41.0
49	34.425611491783215	36.0	34.0	40.0	24.0	41.0
50	34.31742486745715	36.0	34.0	40.0	24.0	41.0
51	33.93968458913585	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	1.0
14	18.0
15	32.0
16	74.0
17	187.0
18	391.0
19	767.0
20	1272.0
21	1945.0
22	3034.0
23	4457.0
24	6215.0
25	8602.0
26	11352.0
27	13546.0
28	15193.0
29	16917.0
30	19782.0
31	23929.0
32	29534.0
33	37836.0
34	63414.0
35	98068.0
36	62287.0
37	80434.0
38	114699.0
39	188252.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.49444030399486	24.85075687141536	25.617224713415805	14.037578111173978
2	32.39678856205648	26.48863010386574	26.497852643753223	14.61672869032456
3	29.750916645687465	25.62632262438589	30.291930776114118	14.330829953812524
4	25.67941454319763	29.01336271252346	29.910441659957478	15.396781084321434
5	22.57204174570217	32.08845662041272	29.818964034587015	15.52053759929809
6	20.826489128619535	40.720753955098694	27.391940497169674	11.060816419112093
7	86.44697911967117	4.11686702842287	7.411806346602989	2.0243475053029605
8	83.8545730088661	6.152555765209089	6.8835043657509765	3.1093668601738322
9	81.24571588096443	5.013447460187292	9.893541978758247	3.847294680090032
10	44.51757392364236	24.73697066983058	16.336732379340514	14.408723027186552
11	37.14353013901109	24.7191487346426	22.267947187249966	15.86937393909634
12	33.180330565740505	22.092718929387747	26.133936204949766	18.593014299921983
13	23.654319264390278	31.502326821887827	27.64979772726706	17.19355618645483
14	19.770508311502503	33.31405739410904	29.39360553950612	17.52182875488234
15	18.573322930973028	23.963523608455823	39.343355160011065	18.119798300560085
16	20.517035526719194	20.931551305986424	38.892073850111295	19.659339317183086
17	21.07312975615106	21.799716344584	28.58164814265524	28.545505756609696
18	23.481209698123838	23.12427247869469	31.58969169298414	21.804826130197338
19	27.36202955699405	25.826102778975603	25.732755719844164	21.079111944186185
20	29.033677226059407	25.189747526739136	24.852750934093738	20.923824313107723
21	25.076335211906546	27.31217799003467	27.48740624789689	20.124080550161892
22	24.40981976165966	23.823814592052166	27.017305971469945	24.74905967481823
23	22.634979348988388	28.275933408276856	25.479385130773125	23.609702111961635
24	22.427222943685177	24.368069074331178	33.27230670678056	19.932401275203084
25	22.17173366301836	25.01190206161155	30.49482665363879	22.321537621731295
26	20.276127829387995	29.281564142764914	27.13034439955034	23.31196362829675
27	21.082975440625535	27.42297309760189	28.048236376189895	23.445815085582677
28	19.430894511591738	27.166985301265484	32.278017203775754	21.124102983367024
29	20.497468786687637	24.433000740295768	31.197858376683424	23.871672096333167
30	21.9121316280774	25.928547749077126	29.325558150606568	22.83376247223891
31	25.97802542928431	25.9147139392459	25.11310074253909	22.99415988893071
32	26.9411577029395	24.61832394046726	26.1386721038109	22.30184625278234
33	24.976382820152995	25.30702333801107	25.762417402184994	23.95417643965094
34	20.79558115710472	26.609270895907432	27.474943356157045	25.120204590830802
35	20.87808550042249	26.65301564591429	29.036792948994368	23.43210590466885
36	26.545710148034228	27.07625544939941	24.348128447547428	22.02990595501893
37	21.656766976327983	29.46526716701023	27.041733239280042	21.836232617381746
38	22.495768848254322	29.830928410657272	24.94485170405119	22.728451037037225
39	22.037009803310642	28.709642040823447	25.383296235458918	23.870051920406986
40	24.14261536275739	25.95858331817015	26.473799262695323	23.425002056377135
41	19.923427993150394	25.14114224895374	27.52404714961203	27.411382608283834
42	23.648087818520356	26.486511412269962	24.997071220441136	24.868329548768543
43	22.049098808298293	25.48212696695589	27.59309156985077	24.87568265489505
44	22.00996532823518	26.87597677914011	27.58175033836751	23.532307554257198
45	20.59106510365387	28.125506304976934	25.87645286160457	25.406975729764625
46	22.732065275641776	27.0050923375649	27.00272438813433	23.260117998658995
47	22.07402459177798	26.052802779723372	28.040010867641595	23.83316176085705
48	22.98992250573916	24.852875563011136	28.582146658324838	23.575055272924867
49	22.482807440844883	23.82481162339135	29.54527893198003	24.147102003783736
50	21.231782367999283	27.44540630273361	27.997761664643523	23.325049664623585
51	20.324483849338595	28.7697131790095	26.519538075380556	24.38626489627135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	869.0
1	1199.0
2	1529.0
3	4537.0
4	7545.0
5	5315.0
6	3085.0
7	3114.0
8	3143.0
9	3452.0
10	3761.0
11	3920.5
12	4080.0
13	3984.5
14	3889.0
15	3873.0
16	3857.0
17	3942.5
18	4028.0
19	3815.5
20	3603.0
21	3589.5
22	3576.0
23	3626.5
24	3677.0
25	3984.5
26	4956.5
27	5621.0
28	6410.0
29	7199.0
30	8035.5
31	8872.0
32	11161.5
33	13451.0
34	15040.0
35	16629.0
36	18597.5
37	20566.0
38	23245.5
39	25925.0
40	33242.0
41	40559.0
42	47846.0
43	55133.0
44	56724.5
45	58316.0
46	62054.0
47	65792.0
48	66757.5
49	67723.0
50	65637.5
51	63552.0
52	59225.0
53	54898.0
54	50728.5
55	46559.0
56	43135.5
57	39712.0
58	37847.5
59	35983.0
60	33688.5
61	31394.0
62	28702.5
63	26011.0
64	23602.5
65	21194.0
66	17808.5
67	14423.0
68	12618.0
69	10813.0
70	9226.5
71	7640.0
72	6560.5
73	5481.0
74	4578.0
75	2942.5
76	2210.0
77	1657.5
78	1105.0
79	809.5
80	514.0
81	404.5
82	295.0
83	202.0
84	109.0
85	81.5
86	54.0
87	40.0
88	26.0
89	17.0
90	8.0
91	6.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	802382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.055150093224864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5475027856511	21.756068300062147
2	5.8910603403819035	3.3054916411531727
3	2.42168169086486	2.0382192994568467
4	1.484010784067138	1.6653658114787147
5	1.1062738596979325	1.551833958901834
6	0.888819577603919	1.4961579993284804
7	0.7319447677089648	1.437437422261793
8	0.6388879610906087	1.4339278112921148
9	0.5440934340475079	1.3738160661246879
>10	8.248828599822414	53.38926425918461
>50	0.4612075217852304	7.862645944077575
>100	0.03202830012167732	1.5408999201146698
>500	0.003202830012167732	0.5826511777241324
>1k	4.5754714459539023E-4	0.5662203888392043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4411	0.5497381546445458	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	834	0.10394051711030408	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08362600357435736	0.0
2	0.0	0.0	0.0	0.2801658063117069	0.0
3	0.0	0.0	0.0	0.41875316245877897	0.0
4	0.0	0.0	0.0	0.7642245214872716	0.0
5	0.0	0.0	0.0	1.2590013235591029	0.0
6	0.0	0.0	0.0	2.160566912019462	0.0
7	0.0	0.0	0.0	2.5404358522499257	0.0
8	0.0	0.0	0.0	3.5060607042530862	0.0
9	0.0	0.0	0.0	3.9025052904975435	0.0
10	0.0	0.0	0.0	4.459970438020793	0.0
11	0.0	0.0	0.0	4.9715721439414144	0.0
12	0.0	0.0	0.0	5.388954388308811	0.0
13	0.0	0.0	0.0	5.582129210276402	0.0
14	0.0	0.0	0.0	5.66650298735515	0.0
15	0.0	0.0	0.0	5.754989518708046	0.0
16	0.0	0.0	0.0	5.930217776570262	0.0
17	0.0	0.0	0.0	6.104947518762883	0.0
18	0.0	0.0	0.0	6.359190510255714	0.0
19	0.0	0.0	0.0	6.471979680501307	0.0
20	0.0	0.0	0.0	6.598727289495527	0.0
21	0.0	0.0	0.0	6.77333240277075	0.0
22	0.0	0.0	0.0	6.960151149951021	0.0
23	0.0	0.0	0.0	7.156067808101378	0.0
24	0.0	0.0	0.0	7.325438506845867	0.0
25	0.0	0.0	0.0	7.469011019688876	0.0
26	0.0	0.0	0.0	7.601740816718221	0.0
27	0.0	0.0	0.0	7.747182763322209	0.0
28	0.0	0.0	0.0	7.887016408643265	0.0
29	0.0	0.0	0.0	8.038689801117174	0.0
30	0.0	0.0	0.0	8.215787492740365	0.0
31	0.0	0.0	0.0	8.375312507010376	0.0
32	0.0	0.0	0.0	8.515769296918425	0.0
33	0.0	0.0	0.0	8.675543569023233	0.0
34	0.0	0.0	0.0	8.83369766520186	0.0
35	0.0	0.0	0.0	9.011169243577248	0.0
36	0.0	0.0	0.0	9.168450937334088	0.0
37	0.0	0.0	0.0	9.324486341916943	0.0
38	0.0	0.0	0.0	9.49310926715704	0.0
39	0.0	0.0	0.0	9.727037745113924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAC	20	7.0320116E-4	45.0	19
CGATATA	25	3.8897495E-5	45.0	10
TATCGAC	20	7.0320116E-4	45.0	15
TAGTACG	20	7.0320116E-4	45.0	1
TAGACGG	65	0.0	44.999996	2
TTAACGG	30	2.1646792E-6	44.999996	2
CGGTCTA	65	0.0	41.53846	31
TCACGAC	60	3.6379788E-12	41.249996	25
TAGCATA	480	0.0	40.781246	30
ATGGGTA	210	0.0	40.714287	5
AACACGT	390	0.0	40.384617	41
CGTTTTT	2660	0.0	40.093987	1
CGTTCGG	45	1.9270374E-8	40.0	45
ACACGAC	410	0.0	39.512196	26
AATGCGG	115	0.0	39.130432	2
TAGGGAC	645	0.0	39.06977	5
TTGATCC	525	0.0	39.0	17
ACACGTG	410	0.0	38.963413	42
CGAGACA	405	0.0	38.88889	22
AGGGTAC	355	0.0	38.66197	6
>>END_MODULE