FastQCFastQC Report
Thu 26 May 2016
SRR1547091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547091_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1564148
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153770.9830911141400941No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.0340687E-445.01
CGTTTTT95400.041.7216951
ACGGGAT4850.038.505155
TACGGGA5400.038.3333324
CGTTCGA601.5643309E-1037.50000414
ATTAGCG900.037.51
GTTGATC9700.037.113416
GGGCGAT37150.037.0053837
GTAGCAT9550.036.9895329
AGGGCGA20550.036.788326
GGGTACC11100.036.4864857
ATAGCGG1850.036.4864852
GGTACCT10400.036.3461578
TAAGGGA13700.036.2956244
GTACGGG4600.036.1956523
TATACGG1750.036.02
AGGGTAC7450.035.9395986
TTACGGG4200.035.8928573
GGCGATT8600.035.8430258
ACGTAGG1950.035.769232
Produced by FastQC (version 0.11.5)