##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547091_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1564148
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46988775998179	31.0	31.0	33.0	30.0	34.0
2	31.82967021023586	31.0	31.0	34.0	30.0	34.0
3	31.889976523960648	31.0	31.0	34.0	30.0	34.0
4	35.67878295404271	37.0	35.0	37.0	33.0	37.0
5	35.62559872850907	37.0	35.0	37.0	33.0	37.0
6	35.670474277370175	37.0	35.0	37.0	33.0	37.0
7	36.02829847303452	37.0	35.0	37.0	35.0	37.0
8	34.710707043067536	37.0	35.0	37.0	33.0	37.0
9	37.01558484235507	39.0	37.0	39.0	34.0	39.0
10	36.91854543176221	39.0	37.0	39.0	32.0	39.0
11	36.84209230840048	39.0	37.0	39.0	32.0	39.0
12	36.61999951411248	39.0	35.0	39.0	32.0	39.0
13	36.58417809567892	39.0	35.0	39.0	32.0	39.0
14	37.69690847669146	40.0	36.0	41.0	33.0	41.0
15	37.81535570802763	40.0	36.0	41.0	33.0	41.0
16	37.84315039241811	40.0	36.0	41.0	33.0	41.0
17	37.72367640402315	40.0	36.0	41.0	33.0	41.0
18	37.61006950748906	39.0	36.0	41.0	33.0	41.0
19	37.512814004812846	39.0	36.0	41.0	32.0	41.0
20	37.35810485964244	39.0	35.0	41.0	32.0	41.0
21	37.26226162741633	39.0	35.0	41.0	32.0	41.0
22	37.169716676427036	39.0	35.0	41.0	32.0	41.0
23	37.079336482225465	39.0	35.0	41.0	32.0	41.0
24	36.92723642519761	39.0	35.0	41.0	31.0	41.0
25	36.821302076274115	38.0	35.0	41.0	31.0	41.0
26	36.72446085664528	38.0	35.0	41.0	31.0	41.0
27	36.615624608413015	38.0	35.0	41.0	31.0	41.0
28	36.62536793193483	38.0	35.0	41.0	31.0	41.0
29	36.55750414922373	38.0	35.0	41.0	31.0	41.0
30	36.469343054493564	38.0	35.0	41.0	31.0	41.0
31	36.341706795009166	38.0	35.0	41.0	30.0	41.0
32	36.19508511982242	38.0	35.0	41.0	30.0	41.0
33	35.97431061510803	38.0	35.0	41.0	29.0	41.0
34	35.72902308477203	38.0	35.0	41.0	27.0	41.0
35	35.5449688904119	38.0	35.0	41.0	27.0	41.0
36	35.40739750969857	38.0	35.0	41.0	26.0	41.0
37	35.356890780156355	38.0	35.0	41.0	26.0	41.0
38	35.227558389615304	38.0	34.0	40.0	25.0	41.0
39	35.1294155028808	38.0	34.0	40.0	25.0	41.0
40	34.991530213253476	38.0	34.0	40.0	24.0	41.0
41	34.897045548119486	38.0	34.0	40.0	24.0	41.0
42	34.83151722215545	38.0	34.0	40.0	23.0	41.0
43	34.73712909520071	38.0	34.0	40.0	23.0	41.0
44	34.62145973398937	37.0	34.0	40.0	23.0	41.0
45	34.49638013794091	37.0	34.0	40.0	23.0	41.0
46	34.41869823060222	37.0	34.0	40.0	23.0	41.0
47	34.334727915772675	37.0	34.0	40.0	23.0	41.0
48	34.25424512258431	37.0	33.0	40.0	23.0	41.0
49	34.176753094975666	36.0	33.0	40.0	23.0	41.0
50	34.07228408053458	36.0	33.0	40.0	23.0	41.0
51	33.718999736597816	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	7.0
13	12.0
14	24.0
15	46.0
16	146.0
17	342.0
18	748.0
19	1466.0
20	2530.0
21	3924.0
22	6026.0
23	8735.0
24	13006.0
25	19277.0
26	26749.0
27	30974.0
28	32402.0
29	33539.0
30	37481.0
31	44030.0
32	53700.0
33	68813.0
34	108317.0
35	152401.0
36	127220.0
37	163257.0
38	235998.0
39	392724.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.920288872919954	23.516572600546752	24.476392259556	17.086746266977293
2	34.76454913473661	25.286737572147906	26.727969476034236	13.22074381708125
3	29.024938816531424	25.48607932241706	32.34431780112879	13.144664059922718
4	25.516063697297188	28.19534980065825	31.38801443341679	14.900572068627776
5	23.179520096563753	31.369857583809207	31.055053613852397	14.395568705774645
6	21.7793329020016	39.603221690019105	28.388554024299488	10.228891383679805
7	85.1813894848825	3.4746711947974234	9.681436794983595	1.6625025253364771
8	82.69613872856021	5.815945805639876	9.233141620869636	2.254773844930275
9	79.95592488690329	5.145484954109202	11.55498073072369	3.3436094282638216
10	39.68556683894363	28.915230528057446	18.13715837631733	13.262044256681593
11	31.340832197464692	26.158905679002242	26.15040264731982	16.34985947621325
12	27.811051128154112	23.319852085608268	30.211271567652165	18.65782521858545
13	23.56720719522705	26.869899779304774	31.401248475208227	18.16164455025995
14	21.038929819940314	27.943583343775654	32.60318077317492	18.414306063109116
15	20.479391975695393	24.041522924940605	37.40982311136798	18.069261987996022
16	23.781892762065993	22.7839053593394	34.765060595288936	18.66914128330567
17	23.14601943038638	22.672470891501316	30.941381506097887	23.240128172014412
18	24.350764761390867	22.76683536340551	32.306213989980485	20.576185885223136
19	26.980055595762035	24.952753831478862	28.77176584312993	19.295424729629165
20	28.857051890230334	24.66448187767398	27.789377987249285	18.689088244846396
21	26.275135089518383	25.284308134524352	29.581024302048142	18.85953247390912
22	25.130102777997994	23.09167674670172	29.330025035994034	22.448195439306254
23	23.331040285190404	26.577664006219358	29.014837470622982	21.076458237967252
24	22.817789620931013	24.76754117896772	32.85264565757204	19.562023542529225
25	23.444904190652036	25.271905216130442	30.39104995179484	20.892140641422678
26	21.57052913151441	27.48947030587898	29.189373384104318	21.75062717850229
27	21.31460705764416	25.874277881632686	30.617690909044416	22.19342415167874
28	21.153688781368515	26.865360566902872	31.796287819311214	20.184662832417395
29	22.168490449752838	25.245948593099886	31.41863813398732	21.166922823159958
30	22.84138073890706	25.966724376465656	31.177292685858372	20.014602198768912
31	24.99092157519621	26.27762845971097	28.386380316952103	20.34506964814071
32	25.791549137293913	26.024966946861806	28.362085940716607	19.82139797512767
33	24.90851249370264	26.326792605303336	27.816996857074905	20.947698043919118
34	22.717351554967944	27.461531773208165	29.55481194874142	20.26630472308247
35	23.153371675826072	27.721289801220856	29.14487631605193	19.980462206901137
36	25.955472244314475	27.91225638494567	26.272577786756752	19.859693583983102
37	24.073361344322915	28.53994634778806	27.13988701836399	20.24680528952503
38	24.524917079457953	28.589685886501787	26.649076685837912	20.236320348202344
39	24.120032119722687	27.29530709370213	26.6415326426911	21.94312814388408
40	25.361091149942332	25.350734073757724	28.02759073949524	21.2605840368047
41	22.0529003649271	26.03628301158202	28.042870623495986	23.867945999994884
42	23.674613911215562	27.011062891746818	27.019118395445958	22.295204801591666
43	22.895723422591725	25.962888422323203	28.37046110726095	22.77092704782412
44	22.54933676352877	27.0815805153988	28.449609627733434	21.919473093338993
45	22.155384273099475	27.63907251743441	27.296138217099656	22.90940499236645
46	23.115076066970644	27.395425496819993	27.675322284080533	21.814176152128827
47	22.6448520216757	27.17409094280081	28.746704276065948	21.434352759457546
48	23.25962760557185	26.08423243836261	29.091876216317125	21.564263739748412
49	23.120126739924867	25.84998350539719	29.124609691666002	21.90528006301194
50	21.886164224868747	27.539401642299833	28.93051041205819	21.643923720773227
51	21.631392937241234	27.51919895048295	28.43682311392528	22.41258499835054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1554.0
1	2300.0
2	3046.0
3	14672.0
4	26298.0
5	18230.0
6	10162.0
7	10248.5
8	10335.0
9	10735.5
10	11136.0
11	11323.5
12	11511.0
13	11353.5
14	11196.0
15	10757.0
16	10318.0
17	10052.0
18	9786.0
19	9473.0
20	9160.0
21	8946.5
22	8733.0
23	9059.5
24	9386.0
25	10412.5
26	12994.0
27	14549.0
28	15571.0
29	16593.0
30	19882.0
31	23171.0
32	26780.0
33	30389.0
34	35577.5
35	40766.0
36	45287.0
37	49808.0
38	54122.5
39	58437.0
40	66546.5
41	74656.0
42	82421.0
43	90186.0
44	94587.5
45	98989.0
46	103188.0
47	107387.0
48	112129.5
49	116872.0
50	113879.5
51	110887.0
52	104153.0
53	97419.0
54	92208.0
55	86997.0
56	83701.0
57	80405.0
58	74923.5
59	69442.0
60	65754.0
61	62066.0
62	56377.0
63	50688.0
64	45934.0
65	41180.0
66	36551.5
67	31923.0
68	26819.0
69	21715.0
70	18825.0
71	15935.0
72	13657.5
73	11380.0
74	9888.0
75	6859.0
76	5322.0
77	3878.0
78	2434.0
79	1776.0
80	1118.0
81	846.5
82	575.0
83	386.5
84	198.0
85	163.5
86	129.0
87	86.0
88	43.0
89	26.0
90	9.0
91	14.0
92	19.0
93	12.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1564148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.07671017908792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00337480083414	21.33924726915614
2	6.0305303626930264	3.386349064390441
3	2.5528822949003835	2.150296089457288
4	1.6241885228949995	1.824074817340952
5	1.2149246972225685	1.70555443066671
6	0.9939034220100746	1.6743322995468348
7	0.8336220156021356	1.6383754611677788
8	0.7601653627768258	1.7074354063092934
9	0.6808886510833269	1.7205401988627018
>10	8.99344980381072	55.03578667253463
>50	0.28085377673816114	4.820085879539757
>100	0.02746094691342728	1.3668676542562013
>500	0.0035206336126735937	0.6177329113964719
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.3470890751157292E-4	1.0133218453748185
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15377	0.9830911141400941	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.278651380815626E-4	0.0	0.0	0.04750189879730051	0.0
2	1.278651380815626E-4	0.0	0.0	0.13828614683520996	0.0
3	1.278651380815626E-4	0.0	0.0	0.24243230180264272	0.0
4	1.278651380815626E-4	0.0	0.0	0.38979687344164365	0.0
5	1.278651380815626E-4	0.0	0.0	0.6301194004659406	0.0
6	1.278651380815626E-4	0.0	0.0	1.1722036533627254	0.0
7	1.278651380815626E-4	0.0	0.0	1.4185997744458965	0.0
8	1.278651380815626E-4	0.0	0.0	1.9039758386035082	0.0
9	1.278651380815626E-4	0.0	0.0	2.1820825139309066	0.0
10	1.278651380815626E-4	0.0	0.0	2.5856248897163185	0.0
11	1.278651380815626E-4	0.0	0.0	2.900620657380248	0.0
12	1.278651380815626E-4	0.0	0.0	3.1679866611087952	0.0
13	1.278651380815626E-4	0.0	0.0	3.3267951626060963	0.0
14	1.278651380815626E-4	0.0	0.0	3.4178990734892096	0.0
15	1.278651380815626E-4	0.0	0.0	3.500883548104144	0.0
16	1.278651380815626E-4	0.0	0.0	3.6201817219342414	0.0
17	1.278651380815626E-4	0.0	0.0	3.7534811283842706	0.0
18	1.278651380815626E-4	0.0	0.0	3.937159399238435	0.0
19	1.278651380815626E-4	0.0	0.0	4.037789262908625	0.0
20	1.278651380815626E-4	0.0	0.0	4.140656766495242	0.0
21	1.278651380815626E-4	0.0	0.0	4.264302355020114	0.0
22	1.278651380815626E-4	0.0	0.0	4.3965149077964485	0.0
23	1.278651380815626E-4	0.0	0.0	4.5484186918373455	0.0
24	1.278651380815626E-4	0.0	0.0	4.676667425333153	0.0
25	1.278651380815626E-4	0.0	0.0	4.784905264719195	0.0
26	1.278651380815626E-4	0.0	0.0	4.886046588941712	0.0
27	1.278651380815626E-4	0.0	0.0	5.004130043960035	0.0
28	1.278651380815626E-4	0.0	0.0	5.120295521907134	0.0
29	1.278651380815626E-4	0.0	0.0	5.242726391620231	0.0
30	1.278651380815626E-4	0.0	0.0	5.40223815137698	0.0
31	1.278651380815626E-4	0.0	0.0	5.527865649542115	0.0
32	1.278651380815626E-4	0.0	0.0	5.66985988538169	0.0
33	1.278651380815626E-4	0.0	0.0	5.802711763848434	0.0
34	1.278651380815626E-4	0.0	0.0	5.93332600239875	0.0
35	1.278651380815626E-4	0.0	0.0	6.079284057518854	0.0
36	1.9179770712234392E-4	0.0	0.0	6.206318072202886	0.0
37	1.9179770712234392E-4	0.0	0.0	6.344540286469055	0.0
38	1.9179770712234392E-4	0.0	0.0	6.503029125121152	0.0
39	1.9179770712234392E-4	0.0	0.0	6.727560307592376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.0340687E-4	45.0	1
CGTTTTT	9540	0.0	41.721695	1
ACGGGAT	485	0.0	38.50515	5
TACGGGA	540	0.0	38.333332	4
CGTTCGA	60	1.5643309E-10	37.500004	14
ATTAGCG	90	0.0	37.5	1
GTTGATC	970	0.0	37.1134	16
GGGCGAT	3715	0.0	37.005383	7
GTAGCAT	955	0.0	36.98953	29
AGGGCGA	2055	0.0	36.78832	6
GGGTACC	1110	0.0	36.486485	7
ATAGCGG	185	0.0	36.486485	2
GGTACCT	1040	0.0	36.346157	8
TAAGGGA	1370	0.0	36.295624	4
GTACGGG	460	0.0	36.195652	3
TATACGG	175	0.0	36.0	2
AGGGTAC	745	0.0	35.939598	6
TTACGGG	420	0.0	35.892857	3
GGCGATT	860	0.0	35.843025	8
ACGTAGG	195	0.0	35.76923	2
>>END_MODULE