FastQCFastQC Report
Thu 26 May 2016
SRR1547090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547090_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences870285
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50510.5803845866583935No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9620.11053850175517216No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG253.890022E-545.01
ATTCGAC207.032341E-445.038
ACGGGAT2100.042.8571435
TCGATAG501.0804797E-940.51
ATAGGGC4400.039.3754
CGTTTTT29600.039.2989851
CGTTAGG2250.039.02
CAATTGC356.2465406E-638.57142625
ATCGTAG356.2465406E-638.5714261
GCGATCG356.2465406E-638.5714269
CGTACAT356.2465406E-638.57142635
TCGTACA356.2465406E-638.57142634
CTAATGG1000.038.252
TAGTAGG4000.038.252
AACACGT4300.038.19767441
CACGTGA4250.038.1176543
ATTAGCG659.094947E-1238.0769271
ACGACCA4550.038.07692328
CGGGTAT950.037.8947376
AGGGCGA9750.037.8461536