FastQCFastQC Report
Thu 26 May 2016
SRR1547089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547089_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1329812
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74240.5582744026975242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGG351.21212E-745.0000042
CTACGAT207.0336874E-445.019
CGTCGTT207.0336874E-445.025
ACGTACT207.0336874E-445.022
GTCGGTT207.0336874E-445.033
TACGGGA2600.043.269234
CGTTTTT55450.041.956721
GCGATAT1350.041.6666649
GTTTGCG1000.040.5000041
AATACGG451.9286745E-840.02
ACGGGAT3200.038.6718755
CGTATGG1750.038.5714262
GGCGATA5400.038.3333328
AACGTAC301.1400184E-437.49999637
ATTACGA301.1400184E-437.49999621
TCGTCGT301.1400184E-437.49999624
ACGGGAC3250.037.3846175
TAGGGCG6050.037.1900835
GCGATAA850.037.0588239
TAAGGGT9900.037.0454524
Produced by FastQC (version 0.11.5)