##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547089_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1329812
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420065392702128	31.0	31.0	33.0	30.0	34.0
2	31.794573969854387	31.0	31.0	34.0	30.0	34.0
3	31.901888387230677	31.0	31.0	34.0	30.0	34.0
4	35.66441045801963	37.0	35.0	37.0	33.0	37.0
5	35.60091802450271	37.0	35.0	37.0	33.0	37.0
6	35.64594844985607	37.0	35.0	37.0	33.0	37.0
7	36.041033619789864	37.0	35.0	37.0	35.0	37.0
8	34.74657319982072	37.0	35.0	37.0	35.0	37.0
9	37.03396871136672	39.0	37.0	39.0	34.0	39.0
10	36.7343940346455	39.0	37.0	39.0	32.0	39.0
11	36.77754750295531	39.0	37.0	39.0	32.0	39.0
12	36.55772319696318	39.0	35.0	39.0	32.0	39.0
13	36.4809709943962	39.0	35.0	39.0	32.0	39.0
14	37.602025699873366	40.0	36.0	41.0	33.0	41.0
15	37.75666936378977	40.0	36.0	41.0	33.0	41.0
16	37.789510096163966	40.0	36.0	41.0	33.0	41.0
17	37.6902441848923	40.0	36.0	41.0	33.0	41.0
18	37.62974315166354	39.0	36.0	41.0	33.0	41.0
19	37.57944656838711	39.0	36.0	41.0	33.0	41.0
20	37.44667516912165	39.0	35.0	41.0	32.0	41.0
21	37.35581345333024	39.0	35.0	41.0	32.0	41.0
22	37.268978622542136	39.0	35.0	41.0	32.0	41.0
23	37.196133739205244	39.0	35.0	41.0	32.0	41.0
24	37.06845403711201	39.0	35.0	41.0	32.0	41.0
25	36.9923853898145	39.0	35.0	41.0	32.0	41.0
26	36.89507614610186	39.0	35.0	41.0	31.0	41.0
27	36.788024171837826	39.0	35.0	41.0	31.0	41.0
28	36.8127419514939	39.0	35.0	41.0	31.0	41.0
29	36.79403479589596	39.0	35.0	41.0	31.0	41.0
30	36.73394284304849	39.0	35.0	41.0	31.0	41.0
31	36.63650049781473	39.0	35.0	41.0	31.0	41.0
32	36.55158774322987	39.0	35.0	41.0	31.0	41.0
33	36.39926997199604	39.0	35.0	41.0	30.0	41.0
34	36.26106096200064	39.0	35.0	41.0	30.0	41.0
35	36.13525069709102	39.0	35.0	41.0	30.0	41.0
36	36.01082559038421	39.0	35.0	41.0	29.0	41.0
37	35.97612970856031	39.0	35.0	41.0	29.0	41.0
38	35.88720210074808	38.0	35.0	41.0	29.0	41.0
39	35.81887289331124	38.0	35.0	41.0	29.0	41.0
40	35.69178124426611	38.0	35.0	41.0	28.0	41.0
41	35.6103133375244	38.0	35.0	41.0	27.0	41.0
42	35.554304668629854	38.0	35.0	41.0	27.0	41.0
43	35.46280978063064	38.0	35.0	40.0	27.0	41.0
44	35.362170742932086	38.0	35.0	40.0	27.0	41.0
45	35.233482627619544	38.0	34.0	40.0	26.0	41.0
46	35.16736576298003	38.0	34.0	40.0	26.0	41.0
47	35.09894857318177	38.0	34.0	40.0	26.0	41.0
48	35.01742426749044	38.0	34.0	40.0	26.0	41.0
49	34.966729131636654	37.0	34.0	40.0	26.0	41.0
50	34.86789034841015	37.0	34.0	40.0	26.0	41.0
51	34.50996531840591	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	3.0
12	2.0
13	6.0
14	15.0
15	24.0
16	92.0
17	219.0
18	522.0
19	1039.0
20	1797.0
21	2879.0
22	4310.0
23	6380.0
24	9542.0
25	13962.0
26	18147.0
27	21281.0
28	22653.0
29	25499.0
30	29314.0
31	35397.0
32	43971.0
33	56651.0
34	90952.0
35	138721.0
36	100584.0
37	131147.0
38	200812.0
39	373657.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.727332886152325	24.266888853461992	28.298736964322774	14.707041296062902
2	32.067765969926576	25.841397129819853	28.821442429456194	13.269394470797376
3	28.869043142940505	25.5039058152581	31.923760651881622	13.703290389919779
4	24.982177931918194	28.61020956345709	31.592661218277474	14.814951286347245
5	22.83405473856455	32.35231747043943	30.38933322905794	14.424294561938078
6	20.860392296053877	40.8911184438101	27.51689712530794	10.731592134828082
7	88.42949228913561	3.308663179456946	6.938800371781875	1.323044159625571
8	86.31107254258497	5.3936947478290165	6.160795661341603	2.1344370482444135
9	82.96984836954397	4.306698992037973	9.759650236274	2.9638024021440628
10	36.62683146189085	24.303886564416626	25.57872842176187	13.490553551930647
11	33.03760230769462	25.432767940129885	24.64055069438387	16.88907905779163
12	30.02055929710365	24.118296420847457	27.190535203472372	18.67060907857652
13	22.933392088505745	30.39910904699311	29.062153146459803	17.605345718041345
14	20.340619576301012	32.25628885887629	29.445816401115344	17.95727516370735
15	20.03576445392281	25.138741416079867	37.28820314450464	17.53729098549269
16	21.290001895004707	22.796906630410916	37.00823875856136	18.904852716023015
17	20.875582413153136	23.886308741386	30.136214743136623	25.10189410232424
18	23.112139159520293	24.113333313280375	32.002042393962455	20.772485133236877
19	26.093237239549648	26.867331622815854	27.01291611145034	20.026515026184153
20	27.033821322111695	26.251379894300847	26.898689438807892	19.816109344779562
21	24.38758260566155	27.70895434843422	29.141487668933653	18.76197537697058
22	23.84359593686927	24.5076747690651	28.404992585418086	23.24373670864754
23	22.046048614390607	28.547117938475512	27.386352356573713	22.02048109056017
24	20.985297169825508	26.869286786402892	33.24387206612664	18.90154397764496
25	21.595834599176424	26.796870535083155	30.84014883306813	20.767146032672287
26	20.119159700769732	30.085305291274256	28.13909033758155	21.65644467037446
27	20.47770662319185	28.762336330248182	29.102685191590993	21.657271854968975
28	19.330175994802275	28.956950305757506	32.637019368151286	19.075854331288934
29	20.061707970750753	26.19625932086641	32.120329790977976	21.621702917404868
30	22.377900033989768	26.926813715021368	30.9729495597874	19.722336691201463
31	24.55790743353196	26.890643188661258	28.243541192288834	20.307908185517952
32	25.472623197865563	26.129633361708272	28.346788869404094	20.05095457102207
33	23.69688346924227	27.141956908194544	27.841529479354975	21.31963014320821
34	20.936418080149675	27.50343657599721	29.64336312200522	21.9167822218479
35	21.25503454623661	27.876572026722574	29.38986864308639	21.478524783954423
36	25.089862326404035	27.088189909551126	27.04961302800697	20.772334736037877
37	21.280602070067047	29.83120922355942	28.32603405594174	20.56215465043179
38	22.3680490174551	29.236989890300286	27.276938394299343	21.118022697945275
39	21.54943706328413	28.903709697310596	26.61030280972047	22.9365504296848
40	23.465647775775825	26.812361446580418	28.07050921483638	21.651481562807373
41	20.518614661320548	26.213254204353696	28.152099695295274	25.11603143903048
42	22.9955061316938	27.52719933343961	26.439902783250563	23.037391751616017
43	21.932348331944667	26.840184928395892	28.488087037867004	22.739379701792434
44	21.77668723097701	28.346864068003597	27.820248275696112	22.056200425323276
45	20.540271857976915	29.40039644701657	27.023519113980022	23.03581258102649
46	23.02859351547437	28.277530959263412	27.29889638535372	21.3949791399085
47	22.50994877471402	27.51246040793736	28.12134346809925	21.856247349249365
48	22.598307129128024	26.032627168351617	29.369264226823038	21.999801475697318
49	21.360387784137906	25.853955295936572	29.717584139712983	23.068072780212542
50	20.988455511004563	28.21932724324942	28.765344274228237	22.02687297151778
51	20.51771227812653	29.18352368605487	27.319275205818567	22.97948883000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1430.0
1	1622.0
2	1814.0
3	7643.0
4	13472.0
5	9626.5
6	5781.0
7	5886.0
8	5991.0
9	6392.5
10	6794.0
11	7088.5
12	7383.0
13	7342.0
14	7301.0
15	7260.0
16	7219.0
17	6991.5
18	6764.0
19	6474.0
20	6184.0
21	6410.0
22	6636.0
23	7057.0
24	7478.0
25	8056.0
26	9919.5
27	11205.0
28	13109.5
29	15014.0
30	18447.0
31	21880.0
32	24573.0
33	27266.0
34	32785.5
35	38305.0
36	41134.0
37	43963.0
38	50346.0
39	56729.0
40	65016.0
41	73303.0
42	83674.5
43	94046.0
44	99470.5
45	104895.0
46	110683.0
47	116471.0
48	117916.5
49	119362.0
50	112624.5
51	105887.0
52	98367.5
53	90848.0
54	83033.0
55	75218.0
56	68190.5
57	61163.0
58	55785.0
59	50407.0
60	44940.5
61	39474.0
62	34430.0
63	29386.0
64	25225.0
65	21064.0
66	17303.0
67	13542.0
68	11403.5
69	9265.0
70	7881.5
71	6498.0
72	5598.0
73	4698.0
74	3947.5
75	2635.5
76	2074.0
77	1502.5
78	931.0
79	648.5
80	366.0
81	308.5
82	251.0
83	199.5
84	148.0
85	90.5
86	33.0
87	22.5
88	12.0
89	12.5
90	13.0
91	11.0
92	9.0
93	6.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1329812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.337911927370566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53716496532084	20.15829110027252
2	6.393805424639284	3.3679896830978726
3	2.6078977831631884	2.06059746385611
4	1.561390417150731	1.6449505316462534
5	1.1643729785481904	1.5333576479806188
6	0.9462644576811914	1.4953577967844975
7	0.797293938217789	1.4699340267504588
8	0.6642394122345955	1.3995743310498532
9	0.5665214861089739	1.3428893705491114
>10	7.834527178077945	48.14255545773134
>50	0.8841536724447624	14.668002526008605
>100	0.039720268512512646	1.6538452254059433
>500	0.0017653452666455648	0.3133797249600588
>1k	5.884484222151883E-4	0.1739706930107151
>5k	2.9422421110759413E-4	0.575304420896013
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7424	0.5582744026975242	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04015605213368506	0.0
2	0.0	0.0	0.0	0.13625986229632459	0.0
3	0.0	0.0	0.0	0.24364346238415654	0.0
4	0.0	0.0	0.0	0.4468300782366229	0.0
5	0.0	0.0	0.0	0.7389766372991069	0.0
6	0.0	0.0	0.0	1.49239140570246	0.0
7	0.0	0.0	0.0	1.8122110493814163	0.0
8	0.0	0.0	0.0	2.57502564272243	0.0
9	0.0	0.0	0.0	2.965306374134088	0.0
10	0.0	0.0	0.0	3.50996983032188	0.0
11	0.0	0.0	0.0	3.9263444757604833	0.0
12	0.0	0.0	0.0	4.258872682755157	0.0
13	0.0	0.0	0.0	4.443410045931304	0.0
14	0.0	0.0	0.0	4.530114031156284	0.0
15	0.0	0.0	0.0	4.608997362033129	0.0
16	0.0	0.0	0.0	4.772403918749418	0.0
17	0.0	0.0	0.0	4.937389646055232	0.0
18	0.0	0.0	0.0	5.161331075370052	0.0
19	0.0	0.0	0.0	5.276760925604521	0.0
20	0.0	0.0	0.0	5.40031222458513	0.0
21	0.0	0.0	0.0	5.557326900343808	0.0
22	0.0	0.0	0.0	5.726222954823689	0.0
23	0.0	0.0	0.0	5.908429161415298	0.0
24	0.0	0.0	0.0	6.056720799631828	0.0
25	0.0	0.0	0.0	6.1823024607989705	0.0
26	0.0	0.0	0.0	6.3079593205656135	0.0
27	0.0	0.0	0.0	6.443316799667923	0.0
28	0.0	0.0	0.0	6.581306229752777	0.0
29	0.0	0.0	0.0	6.725612342195739	0.0
30	0.0	0.0	0.0	6.919549530309547	0.0
31	0.0	0.0	0.0	7.080850526239799	0.0
32	0.0	0.0	0.0	7.239895564185012	0.0
33	0.0	0.0	0.0	7.39683504134419	0.0
34	0.0	0.0	0.0	7.551217766120324	0.0
35	0.0	0.0	0.0	7.727558481950832	0.0
36	0.0	0.0	0.0	7.877053297759383	0.0
37	0.0	0.0	0.0	8.046776536833779	0.0
38	0.0	0.0	0.0	8.215296598316153	0.0
39	0.0	0.0	0.0	8.413444908002033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCGG	35	1.21212E-7	45.000004	2
CTACGAT	20	7.0336874E-4	45.0	19
CGTCGTT	20	7.0336874E-4	45.0	25
ACGTACT	20	7.0336874E-4	45.0	22
GTCGGTT	20	7.0336874E-4	45.0	33
TACGGGA	260	0.0	43.26923	4
CGTTTTT	5545	0.0	41.95672	1
GCGATAT	135	0.0	41.666664	9
GTTTGCG	100	0.0	40.500004	1
AATACGG	45	1.9286745E-8	40.0	2
ACGGGAT	320	0.0	38.671875	5
CGTATGG	175	0.0	38.571426	2
GGCGATA	540	0.0	38.333332	8
AACGTAC	30	1.1400184E-4	37.499996	37
ATTACGA	30	1.1400184E-4	37.499996	21
TCGTCGT	30	1.1400184E-4	37.499996	24
ACGGGAC	325	0.0	37.384617	5
TAGGGCG	605	0.0	37.190083	5
GCGATAA	85	0.0	37.058823	9
TAAGGGT	990	0.0	37.045452	4
>>END_MODULE